FastQCFastQC Report
Tue 10 May 2022
EGAF00003656672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707512
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA155410.5739956092530707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71830.26529891649603027Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62390.23043295837654643No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59400.21938960935353197Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53290.19682276569780668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35590.13144909422377443Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG30740.11353596955433623No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT29510.10899305340105601Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27680.10223408058763912Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)