FastQCFastQC Report
Tue 10 May 2022
EGAF00003656963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72542
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATG30294.175512117118359No Hit
ATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCG23773.276722450442502No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATG19562.69636900002757No Hit
ATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCG17572.422045160045215No Hit
ACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCGTC14882.051225496953489RNA PCR Primer, Index 19 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13781.8995892034959057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCGTC13161.8141214744561773No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8611.1868986242452648Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7891.0876457776184831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5070.698905461663588Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4950.6823633205591244Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCGTCTTC4070.5610542857930578RNA PCR Primer, Index 19 (96% over 25bp)
CACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCGTCT4040.556918750516942RNA PCR Primer, Index 19 (95% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3980.5486476799647101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCGTCT3730.5141848859970776No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3550.48937167434038215Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3330.45904441564886544Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2680.36944115133302086Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.3625485925394944No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2500.34462793967632543Clontech SMART CDS Primer II A (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGC2410.33222133384797775No Hit
CATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCGTCTTC2310.31843621626092466RNA PCR Primer, Index 37 (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2260.3115436574673982Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2240.3087866339499876No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2180.30051556339775576Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2040.28121639877588156Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.26605276943012324No Hit
TATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCC1930.26605276943012324No Hit
TCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCGTCTTCTG1880.2591602106365967RNA PCR Primer, Index 37 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGC1860.25640318711918614No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1730.23848253425601723No Hit
ACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTATGCCGTCTT1660.2288329519450801RNA PCR Primer, Index 37 (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1630.22469741666896417Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1610.22194039315155356Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1560.2150478343580271Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1530.21091229908191117Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1520.20953378732320588Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1510.20815527556450059Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1510.20815527556450059Clontech Universal Primer Mix Long (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCC1510.20815527556450059No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1490.20539825204708997Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1430.19712718149485814Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1350.18609908742521572Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1350.18609908742521572Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1220.1681784345620468Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.16679992280334152Clontech Universal Primer Mix Long (95% over 22bp)
ACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCGTCTT1210.16679992280334152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.159907364009815No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1070.14750075818146727Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1060.14612224642276198No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1060.14612224642276198Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1040.1433652229053514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1020.14060819938794078Clontech Universal Primer Mix Long (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGCGTCG1010.1392296876292355No Hit
TCTCCGAGCCCACGAGACAGCGTCGTGAATATCGCGTATGCCGTCTTCTG1000.13785117587053017RNA PCR Primer, Index 44 (95% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC900.12406605828347715Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG850.11717349948995065No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG830.11441647597254005Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA810.11165945245512944Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA800.11028094069642413Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT780.10752391717901355Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA780.10752391717901355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC780.10752391717901355Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA730.10063135838548702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG730.10063135838548702No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA730.10063135838548702Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)