FastQCFastQC Report
Tue 10 May 2022
EGAF00003656969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46909
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7191.532754908439745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4560.9720949071606727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2990.6374043360549148Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2860.6096911040525272Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2710.5777142978959262Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1820.38798524803342643Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1740.3709309514165725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTGTTGTT1620.34534950649129165No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.29631840371783663Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1330.28352768125519623Clontech Universal Primer Mix Long (100% over 23bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG930.19825619817092666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG840.17907011447696605Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC710.15135688247457843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT710.15135688247457843Clontech SMART CDS Primer II A (100% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG660.14069794708904476Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA600.12790722462640433Clontech Universal Primer Mix Long (95% over 22bp)
CTGCTGCGCCGCTTCTACCGCCGCGACGATCTCGACCAGATGCTGGTGAC600.12790722462640433No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG550.11724828924087062Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG530.11298471508665714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCATCGTCACCGCGCATCAGGCGTTCTTCACGCGCGAGGCGCTGGCGGG510.10872114093244366No Hit
CTGCTGCGGCGCGGAGACGCAGCCCGCGAGCGCCAGCGTGGCGACGGCGG490.10445756677823019No Hit
CGCGTAATCCGCCGGTGCGGTTTCGCAAAAGCGTCCCGACGTCGTGGCTG470.10019399262401671No Hit
GCCGAGTGAGATCAGCCAGGTGCCCTTCTCCCTGGCACAGGACCGGAATA470.10019399262401671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)