FastQCFastQC Report
Tue 10 May 2022
EGAF00003656975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201323
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA364503.034154844284177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA193241.6085598960479404Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG157511.3111378039045285Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG152941.2730964112066447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT118250.9843314412526856Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG113700.9464565316738296Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCTCGTATG102160.8503957719947091No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC101740.8468996264951225Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA97880.8147683845227304Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97520.8117716883802275Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT89190.7424314693050912Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA77010.6410432498170766Clontech Universal Primer Mix Long (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCTCGTATGCCG64560.5374075082221851No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA64560.5374075082221851Clontech Universal Primer Mix Long (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCGCGTATG59640.4964526609413122No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT58590.48771229719234543Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA56480.47014832813489793Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT48320.4022232155714991Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA43380.3611018851715983Clontech Universal Primer Mix Long (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCTCGTATGCCGTC43290.36035271113597256No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT41350.34420384859026254Clontech Universal Primer Mix Long (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCGCGTATGCCG38960.3243091158664239No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC38290.318731931378988Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC36420.30316575974987575Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT35680.2970058843458421Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA35170.2927605648106296Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT32470.270285343741858Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCGCGTATGCCGTC29320.2440642524949576No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT28880.240401623876343Clontech SMART CDS Primer II A (100% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26680.22208848078326976Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG26530.22083985739056025Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACAGCCTCAACAGCATCTCGTATGCCGTCTTC26280.21875881840271102RNA PCR Primer, Index 43 (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG24510.20402506236873846Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24260.20194402338088924Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23360.194452283024632Clontech Universal Primer Mix Long (95% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23050.19187179467969898Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC22710.189041581656224Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22620.1882924076205983Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20580.17131112947974858Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20370.16956305672995522Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20280.16881388269432948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG19490.16223779949272593Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA19140.15932434490973701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT18090.15058398116077024Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT17940.1493353577680607Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG17920.14916887464903278No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA17440.14517327979236228Clontech Universal Primer Mix Long (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17410.14492355511382035Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT17070.14209334209034538Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA16710.13909664594784252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.13135518091304338Clontech SMART CDS Primer II A (100% over 23bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG15410.1282752432110265Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAA14360.11953487946205976Clontech Universal Primer Mix Long (95% over 21bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT14280.11886894698594798Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.11570576772441718No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13840.11520631836733336Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.1132085209389981Clontech SMART CDS Primer II A (100% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13490.11229286378434443Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA13330.11096099883212093Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.1102118247964952No Hit
CATCTCCGAGCCCACGAGACAGCCTCAACAGCATCGCGTATGCCGTCTTC13170.10962913387989742RNA PCR Primer, Index 43 (95% over 23bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC12980.108047544249132Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12440.10355250003537766Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12360.10288656755926592Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA12190.10147146104752844Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12090.10063904545238875Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACAGCCTCAACAGCATCTCGTATGCCGTCT12050.10030607921433286RNA PCR Primer, Index 43 (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.10022283765481889Clontech SMART CDS Primer II A (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)