FastQCFastQC Report
Tue 10 May 2022
EGAF00003656989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132355
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA98687.455706244569529Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39122.9556873559744625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36602.765290317706169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28902.1835215896641604Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28112.123833629254656Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23791.7974387065090098Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20531.5511314268444714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA16391.2383362925465604Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13351.0086509765403648Clontech Universal Primer Mix Long (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCTCGTATGCCG11700.8839862491027917No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10260.7751879415209096Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9550.7215443315326207Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8910.6731895281628952Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8670.6550564768992483Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA8220.6210570057799101Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA7550.5704355710022289Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7340.5545691511465377Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7220.5455026255147142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7060.5334139246722829Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7030.531147293264327Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCGCGTATGCCG6500.49110347172377317No Hit
ACACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCTCGTATGCCGTC6290.475237051868082No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6160.4654149824336066Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5870.4435042121566998Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5840.4412375807487439Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.4382154055381361Clontech Universal Primer Mix Long (95% over 22bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5670.4283933361036606Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5100.38532733935249897Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT4540.34301688640398925Clontech SMART CDS Primer II A (100% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4450.33621699218012163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA4210.31808394091647463Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4090.30901741528465115Clontech Universal Primer Mix Long (95% over 22bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4040.30523969627139136Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4040.30523969627139136Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3890.293906539231612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA3680.2780401193759208Clontech Universal Primer Mix Long (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCGCGTATGCCGTC3550.2682180499414454No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3520.26595141853348947Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC3490.2636847871255336Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3430.25915152430962185Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3370.2546182614937101Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3250.24555173586188658Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3190.24101847304597485Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2920.22061879037437196Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT2800.21155226474254846Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2780.21004117713724454Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT2680.20248573911072495Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGCTGCCTATCCGATCTCGTATGCCGTCTTC2590.1956858448868573RNA PCR Primer, Index 9 (95% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2580.19493030108420534Clontech Universal Primer Mix Long (95% over 22bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2580.19493030108420534Clontech Universal Primer Mix Long (96% over 25bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2500.1888859506629897Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1737750746099505Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT2260.17075289939934266Clontech Universal Primer Mix Long (96% over 26bp)
GGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2170.16395300517547504Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT2160.16319746137282307Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2120.16017528616221524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1990.1503532167277398Clontech Universal Primer Mix Long (96% over 26bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1970.14884212912243588Clontech Universal Primer Mix Long (95% over 21bp)
CACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCTCGTATGCCGTCT1900.14355332250387218TruSeq Adapter, Index 9 (95% over 21bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1890.1427977787012202Clontech Universal Primer Mix Long (96% over 28bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1890.1427977787012202Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGCTGCCTATCCGATCTCGTATG1880.14204223489856826No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA1850.13977560349061238Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1780.13448679687204868Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.13297570926674473Clontech SMART CDS Primer II A (100% over 23bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.13070907785878888Clontech Universal Primer Mix Long (95% over 21bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1710.12919799025348497Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.12390918363492125Clontech SMART CDS Primer II A (100% over 24bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC1620.12239809602961732Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1540.11635374560840166Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1540.11635374560840166Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.1118204827924899Clontech SMART CDS Primer II A (100% over 21bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT1430.10804276377923011Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTA1420.10728721997657815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1360.1027539571606664Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1350.10199841335801443Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1350.10199841335801443Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)