FastQCFastQC Report
Tue 10 May 2022
EGAF00003656993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635798
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA391656.159975338079075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATG207393.2618850641241397No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG189922.9871122589250043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG152182.393527504018572Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC146932.3109541080657694Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCCG126991.997332486104077No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG118801.868517988417705Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT105881.6653087930443315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA79051.2433194190607708Clontech Universal Primer Mix Long (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCGCGTATG74951.178833528888106No Hit
ACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCCGTC67121.0556812069242119RNA PCR Primer, Index 5 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT59200.9311133410296981Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG56830.8938373508567186Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCGCGTATGCCG53580.8427204866954598No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT48960.7700558982569936Clontech Universal Primer Mix Long (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48400.7612480693553613Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47080.7404867583729423Clontech Universal Primer Mix Long (95% over 22bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT43800.6888980462348104Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC43240.6800902173331781Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG41150.6472181416110149Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40120.6310180277383697Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA39830.6264568306285959Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT36090.5676331161784088Clontech SMART CDS Primer II A (100% over 26bp)
ACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCGCGTATGCCGTC34210.5380639762943576No Hit
GCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGT33250.5229648410344165Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA31190.4905646132891264Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30800.48443058958977536Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCT29960.47121884623732696Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG29280.460523625428202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG28200.44353709826076837Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT27140.4268651364112501Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27020.4249777445037575Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCCGTCTTC25580.4023290416138459RNA PCR Primer, Index 5 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA23590.3710297924812598Clontech Universal Primer Mix Long (95% over 21bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC22970.36127826762588117Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA22870.359705441036304Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT22440.35294228670112204Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.34224706589199716No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.3376858687822233Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC21420.33689945548743466Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCCGTCT20020.314879883233354TruSeq Adapter, Index 5 (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT19730.31031868612358016Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA19200.30198270519882103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT18820.29600596415842767Clontech SMART CDS Primer II A (100% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA18080.28436704739555646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16170.25432605953463205Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.25416877687567435No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA15600.2453609479740421Clontech Universal Primer Mix Long (95% over 22bp)
CTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGC14870.23387931387012859Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGC14670.23073366069097417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA14120.22208311444829962Clontech Universal Primer Mix Long (95% over 22bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13880.21830833063331434Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13680.21516267745415996Clontech Universal Primer Mix Long (95% over 23bp)
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12650.19896256358151487Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12620.19849071560464174Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.19314310520007927No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11910.18732364681864366Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTC11540.18150418843720803Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCC11490.18071777514241943Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTG11190.17599929537368786Clontech Universal Primer Mix Long (95% over 23bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11090.17442646878411067Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11060.1739546208072375Clontech Universal Primer Mix Long (96% over 26bp)
CTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11020.17332549017140667Clontech Universal Primer Mix Long (96% over 26bp)
CTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTA11010.17316820751244894Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10950.1722245115587026Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10670.16782059710788647Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10650.16750603178997103Clontech Universal Primer Mix Long (96% over 25bp)
AAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATAC10630.1671914664720556Clontech SMART CDS Primer II A (96% over 26bp)
CATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCGCGTATGCCGTCTTC10550.16593320520039384Illumina PCR Primer Index 5 (95% over 23bp)
CACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCGCGTATGCCGTCT10490.16498950924664751Illumina PCR Primer Index 5 (95% over 21bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10280.16168657340853543No Hit
AAAAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT10120.1591700508652119Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10070.1583836375704233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATAC9550.15020493930462192Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCC9440.148474830056087No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT9320.14658743814859435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT9290.1461155901717212Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8930.1404534144492433Clontech Universal Primer Mix Long (95% over 22bp)
ACATCTCCGAGCCCACGAGACGGCGGTTCAGTGATCTCGTATGCCGTCTT8910.14013884913132788Illumina PCR Primer Index 5 (100% over 22bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA8760.1377796092469621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG8530.13416210809093454Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA8450.13290384681927278Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.13180286820656875Clontech SMART CDS Primer II A (100% over 23bp)
AAAAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCT7900.12425330057659822Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT7560.11890569017203577Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7450.11717558092350086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.11607460231079683Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA7300.11481634103913507No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7100.11167068785998069Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7040.11072699190623438No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7010.11025514392936121No Hit
AAAGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTAC6960.10946873063457262Clontech SMART CDS Primer II A (96% over 26bp)
GTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCA6660.10475025086584104Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA6570.10333470693522157No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6560.10317742427626383Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT6550.10302014161730612Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.10254829364043296Clontech SMART CDS Primer II A (100% over 24bp)
GTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6440.10129003236877122Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA6410.10081818439189806Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC6400.10066090173294034Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)