FastQCFastQC Report
Tue 10 May 2022
EGAF00003657029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586489
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA531973.353127566595167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG240791.5177539837969252Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG190141.1984955458247741Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA170351.0737546872370374Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG142180.8961927879739475Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC138790.8748248490849921Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT128980.8129901940700502Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT113600.7160465657183882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT98010.6177792597364369Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA92070.5803380924796832Clontech Universal Primer Mix Long (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCTCGTATG84890.5350809239774118No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA68070.42906064901805185Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA64150.4043519999193187Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60030.3783827054584053Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC56760.35777115378675806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCTCGTATGCCG55680.3509636688309847No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC54940.34629928099091767Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA53970.3401851509843434Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT48600.3063368230098034Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA48100.30318520960435275Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG48060.3029330805319167Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA45260.285284045461393Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT42500.26788713946330545Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCGCGTATG42260.2663743650286891No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41700.2628445580145844Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCTCGTATGCCGTC36780.23183268210495RNA PCR Primer, Index 45 (95% over 21bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC36460.22981564952546155Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG34890.2199195834323465Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA32980.20788042022352504Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31500.19855164454339108Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30140.18997925608056532Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG29190.18399119061020908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT29170.18386512607399105Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28580.1801462222555593Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT28550.17995712545123224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCGCGTATGCCG27950.17617518936469148No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA27940.17611215709658246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT27940.17611215709658246Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG27060.1705653175029893Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT26190.16508151017750516Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA26160.16489241337317811Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT24580.15493331501195406Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24250.15285325016435664Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA22260.14030982881066303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21680.13665395726034027Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20790.13104408539863813Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACTGTGAATCAATCATCTCGTATGCCGTCTTC19690.12411053590664668RNA PCR Primer, Index 45 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTGTGAATCAATCATCGCGTATGCCGTC19640.12379537456610162No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC18380.11585330878436599Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC18250.1150338892989488Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17790.11213440496593421Clontech Universal Primer Mix Long (95% over 22bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC17780.1120713726978252Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17600.11093679187186296Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT15930.1004104030976578Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)