FastQCFastQC Report
Tue 10 May 2022
EGAF00003657033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913013
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA568086.222036268924977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG247712.713104851738146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG226072.4760874160608886Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATG189842.0792694079930953No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC185732.034253619608921Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT182772.001833489775063Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG179461.9655798986432833Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA141461.5493755291545686Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT139021.5226508275347668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGCCG127561.3971323518942227No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT106981.1717248275763872Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCGCGTATG100281.0983414255875874No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA87690.9604463463280369Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT78010.8544237595740696Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGCCGTC71360.7815879949135445No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC71230.7801641378600305Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT66720.7307672508496593Clontech Universal Primer Mix Long (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCGCGTATGCCG63700.6976899562218719No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG61060.6687747052889718Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC57870.6338354437450507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56460.6183920710877063Clontech Universal Primer Mix Long (95% over 22bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA55240.6050297202778054Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG55060.6030582258960168Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT55000.6024010611020872Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA54260.5942960286436227Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA50820.5566185804583287Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG49450.5416133176636039Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA48620.5325225380142452Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA48260.5285795492506679Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT47280.5178458576164853Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43440.475787310804994Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40160.4398623020701787Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCGCGTATGCCGTC39980.43789080768839No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA39400.4315382146804043Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC39090.42814286324510165Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA37090.4062373701141167Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG36760.40262296374750417Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG34460.3774316466468714Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT32480.35574520844719626Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32380.354649933790647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT30770.33701601182020413Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30310.33197774840007754Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT29950.32803475963650025Clontech SMART CDS Primer II A (100% over 26bp)
CATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGCCGTCTTC28730.3146724088265994RNA PCR Primer, Index 9 (95% over 22bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC27740.3038291897267618Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT27210.2980242340470508Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG26820.29375266288650875Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC26580.29112400371079056Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA26520.290466838916861Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC26500.2902477839855511Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA26400.2891525093290019Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT25930.2840047184432204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.26352308236574945No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC23070.2526798632659119Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21170.23186964479147612Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.22978862294403254No Hit
CACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGCCGTCT20670.2263932715087299No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20430.22376461233301168Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA19640.2151119425462726Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.18926346065171032No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.1615530118410143Clontech SMART CDS Primer II A (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT14520.15903388013095104Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT14430.1580481329400567Clontech Universal Primer Mix Long (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGC14370.15739096814612716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT14010.15344797938254987Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC13700.1500526279472472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.14786207863414869Clontech SMART CDS Primer II A (100% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA13380.1465477490462896Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.14413814480188125No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.14249523281705737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT12730.13942846377871948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12280.13449972782424785Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG12160.13318539823638875Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.13187106864852965No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.129899574266741Clontech SMART CDS Primer II A (100% over 24bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA11230.12299934393048073Clontech Universal Primer Mix Long (95% over 22bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11210.12278028899917089Clontech Universal Primer Mix Long (95% over 22bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT11070.12124690448000193Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11050.12102784954869207Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT10970.12015162982345268Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCGCGTATGCCGTCT10950.11993257489214283No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10800.11828966290731895Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10800.11828966290731895Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10700.11719438825076971No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10320.11303234455588257Clontech SMART CDS Primer II A (100% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10310.11292281709022764Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT9570.10481778463176318Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACGATGTTAGTCCGATCGCGTATGCCGTCTTC9390.10284629024997452Illumina Paired End PCR Primer 2 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACATC9380.1027367627843196Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9310.10197007052473513Clontech Universal Primer Mix Long (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACGATGTTAGTCCGATCTCGTATGCC9250.10131290573080559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)