FastQCFastQC Report
Tue 10 May 2022
EGAF00003657069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620529
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6447210.38984479371633Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT450517.260095821468457Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG222133.5796876535987843Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG166062.676103775971792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC140272.2604906458843983Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC125692.0255298301932707Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG119991.9336727211782205Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT105151.6945219320934235Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC101571.6368292215190587Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77461.2482897656676804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG68381.101963002534934Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG54170.8729648412886424Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG52840.8515315158517974Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT52090.8394450541392909Clontech Universal Primer Mix Long (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC51570.8310651073519529Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA48390.7798185096909249Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA46510.7495217789982418Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG46120.7432368189077384Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC46010.7414641378565708Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC42050.6776476200145359Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40310.6496070288415207Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC37580.6056123082079967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36160.5827286073656509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA31820.5127882822559462Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA29760.47959080075226135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG27310.44010835915807317Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC27280.43962490068957294Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT27250.4391414422210727Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24760.39901438933555083Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC22170.35727580822169475Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19450.31344224041100416Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT19110.3079630444346678Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT19000.3061903633835002Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17360.27976130043881914Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.26976982542314704No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA16370.2638071709783104Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16250.26187333710430943No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16170.26058411452164204Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15600.251398403620137Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG14790.2383450249706299Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14710.23705580238796253Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12930.20837059992361356Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT12610.20321370959294408Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA12510.20160218136460986Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG12360.19918488902210857Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10610.17098314502625986Clontech SMART CDS Primer II A (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10610.17098314502625986Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10370.16711547727825776Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10260.1653427962270901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10020.16147512847908801Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.14535984619574588No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA9020.14535984619574588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA8730.14068641433357668Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8630.13907488610524246Clontech Universal Primer Mix Long (96% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8620.13891373328240905Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.13278992601473905No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG7700.1240876735817343Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG7650.12328190946756719Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC7330.11812501913689771Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG6870.11071198928656034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6630.10684432153855823No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA6410.10329895943622296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT6410.10329895943622296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC6370.10265434814488929Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA6230.10039820862522138Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)