FastQCFastQC Report
Wed 8 May 2024
EGAF00003657071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15625413
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG276610.17702572085614635No Hit
CTCCAGAGTCGCCCTGGCATGAGTCCCTCCGGGTGTTCCCGGCACACAGC220910.14137866307917749No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA206570.13220130565508892Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG172520.11040988164600833No Hit
GTGTAGAACTGTGGGTGCACGATGATCCTGCTGACCGGCAGCAGCTGGTC162250.10383725537366596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)