FastQCFastQC Report
Tue 10 May 2022
EGAF00003657443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54602
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6351.1629610636973005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4990.9138859382440203No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3940.7215852899161203No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3620.6629793780447603Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3060.5604190322698802No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2730.49998168565254014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.44687007801912015No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2200.40291564411560016Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1910.3498040364821801No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1910.3498040364821801No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1780.3259953847844401No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1750.32050108054650017Clontech Universal Primer Mix Long (96% over 26bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1730.31683821105454013No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1640.3003552983407201No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1470.2692209076590601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.2692209076590601No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1450.2655580381671001No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1450.2655580381671001No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1440.26372660342112014No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1390.2545694296912201No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1370.2509065601992601No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1360.2490751254532801No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1330.2435808212153401Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1270.23259221273946007Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1250.2289293432475001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.21061499578770007No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT1090.19962638731182009No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1090.19962638731182009No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1030.18863777883594007No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG960.17581773561408007No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA930.17032343137614006No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC920.16849199663016007Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT900.16482912713820008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA900.16482912713820008No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA860.15750338815428006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG860.15750338815428006No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC860.15750338815428006No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA810.14834621442438006No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG810.14834621442438006No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG810.14834621442438006No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC790.14468334493242005No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC780.14285191018644006No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC780.14285191018644006No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTACCTCAATAATCTCGTATG770.14102047544046006No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA740.13552617120252006No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT730.13369473645654004No Hit
GGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATG720.13186330171056007No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG700.12820043221860006No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT700.12820043221860006No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA690.12636899747262004No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA690.12636899747262004No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC690.12636899747262004No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC680.12453756272664004No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA680.12453756272664004No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA660.12087469323468006No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC650.11904325848870005No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG650.11904325848870005Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTACATTTGCTATGCGTCTCAAGATAAGGATATTATCTCATATGGCG640.11721182374272004No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG640.11721182374272004No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG630.11538038899674005No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC630.11538038899674005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG620.11354895425076005No Hit
ACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACG620.11354895425076005No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG620.11354895425076005Clontech Universal Primer Mix Long (96% over 26bp)
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG620.11354895425076005No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT610.11171751950478004No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA610.11171751950478004No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.11171751950478004No Hit
ATACACATCTCCGAGCCCACGAGACTCTACCTCAATAATCTCGTATGCCG600.10988608475880003No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT600.10988608475880003Clontech Universal Primer Mix Long (96% over 26bp)
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT600.10988608475880003No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG600.10988608475880003No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC590.10805465001282005No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA590.10805465001282005No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT580.10622321526684005No Hit
CAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGAT570.10439178052086004No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA570.10439178052086004No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT570.10439178052086004No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA570.10439178052086004No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG560.10256034577488003No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG560.10256034577488003No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC560.10256034577488003No Hit
GCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGC550.10072891102890004No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC550.10072891102890004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA550.10072891102890004No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA550.10072891102890004No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA550.10072891102890004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)