FastQCFastQC Report
Wed 11 May 2022
EGAF00003657479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013700
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG167760.41796845803124294No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG159330.3969653935271694No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG134440.3349527867055336No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC126760.3158183222463064No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC96680.24087500311433338No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA93880.2338988962802402No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA93860.23384906694571095No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC86400.2152627251663054No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT67940.16927024939581933No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT61050.15210404365049704No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG60700.1512320302962354No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA59720.14879039290430277No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG57890.14423100879487755No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC56040.1396217953509231No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT54660.13618357126840572No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC54270.1352118992450856No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT51960.12945661110695866No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG49750.12395046964147793No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG47160.11749757081994171No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA46480.11580337344594763No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT44200.11012282930961458No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG43680.10882726661185439No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA43300.10788050925579888No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC42540.10598699454368787No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT42270.10531429852754318No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG41880.10434262650422305No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC40800.10165184243964422No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG40320.10045593841094252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)