FastQCFastQC Report
Tue 10 May 2022
EGAF00003657488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26538
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2360.8892908282462882No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2350.8855226467706685No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1620.6104453990504183No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1450.5463863139648806No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1160.4371090511719044No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA1090.4107317808425654No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1070.4031954178913256No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1060.39942723641570577No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC910.3429045142814078No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT900.3391363328057879No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC900.3391363328057879No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT890.33536815133016806No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC790.29768633657396937No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG790.29768633657396937No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT770.29014997362272965No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT770.29014997362272965No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA760.2863817921471098No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA740.2788454291958701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG710.2675408847690105No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT700.2637727032933906No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT700.2637727032933906No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA680.25623634034215087No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT670.252468158866531No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT640.2411636144396714No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG640.2411636144396714No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT600.22609088853719198No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG540.20348179968347277No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA540.20348179968347277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.19971361820785288No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT520.19594543673223302No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG500.18840907378099328No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC500.18840907378099328No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC500.18840907378099328No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC500.18840907378099328No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC490.18464089230537342No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC490.18464089230537342No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC490.18464089230537342No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT490.18464089230537342No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA480.18087271082975356No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA480.18087271082975356No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC470.1771045293541337No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT470.1771045293541337No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG460.17333634787851382No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTC460.17333634787851382No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT460.17333634787851382No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA460.17333634787851382No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT460.17333634787851382No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC450.16956816640289396No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC440.1657999849272741No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC440.1657999849272741No Hit
GGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGG440.1657999849272741No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT440.1657999849272741No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC440.1657999849272741No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG440.1657999849272741No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG430.16203180345165424No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG430.16203180345165424No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG420.15826362197603439No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC420.15826362197603439No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG420.15826362197603439No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG410.1544954405004145No Hit
ACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTA410.1544954405004145No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA410.1544954405004145No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG410.1544954405004145No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA400.1507272590247946No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT400.1507272590247946No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC400.1507272590247946No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT390.14695907754917475No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA390.14695907754917475No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT380.1431908960735549Clontech Universal Primer Mix Long (96% over 26bp)
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC380.1431908960735549No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC380.1431908960735549No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA380.1431908960735549No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT370.13942271459793504No Hit
ATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCA370.13942271459793504No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG370.13942271459793504No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC360.13565453312231518No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC350.1318863516466953No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC350.1318863516466953No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC350.1318863516466953No Hit
GTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTC350.1318863516466953No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC350.1318863516466953No Hit
GGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATC350.1318863516466953No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG350.1318863516466953Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG340.12811817017107544No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG340.12811817017107544No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG340.12811817017107544No Hit
GCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGC330.12434998869545558No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC330.12434998869545558No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT320.1205818072198357No Hit
GCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGT320.1205818072198357No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA320.1205818072198357No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT320.1205818072198357No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA320.1205818072198357No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG310.11681362574421585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT310.11681362574421585No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA310.11681362574421585No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA310.11681362574421585No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG310.11681362574421585No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG310.11681362574421585No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC310.11681362574421585No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA300.11304544426859599No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA300.11304544426859599No Hit
CAATAAGAGCAGGGAAGAACTGCGGATGGCGCTTGAGCACCGCATCGGCT300.11304544426859599No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT300.11304544426859599No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG300.11304544426859599No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT300.11304544426859599No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC300.11304544426859599No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA300.11304544426859599No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC290.1092772627929761No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA290.1092772627929761No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA290.1092772627929761No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT290.1092772627929761No Hit
GAGTACATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA290.1092772627929761No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA290.1092772627929761No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA290.1092772627929761No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG290.1092772627929761No Hit
ATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTG290.1092772627929761No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA290.1092772627929761No Hit
GTGTTACAGCATAGCTGTTAAAGAAGCCGCCACTTCCACCTCAAAGGTAG280.10550908131735624No Hit
ATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAA280.10550908131735624No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA280.10550908131735624No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG280.10550908131735624No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG280.10550908131735624No Hit
CTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGC280.10550908131735624No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT280.10550908131735624No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG280.10550908131735624No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC280.10550908131735624No Hit
GACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA280.10550908131735624No Hit
GGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTC270.10174089984173638No Hit
AGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCT270.10174089984173638No Hit
GAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAAC270.10174089984173638No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC270.10174089984173638No Hit
GCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAA270.10174089984173638No Hit
CACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGA270.10174089984173638No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC270.10174089984173638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)