FastQCFastQC Report
Tue 10 May 2022
EGAF00003657519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173259
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC24690.21043946818221723No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG23230.19799549801024327No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20170.1719143002525444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT19700.16790836464923772No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC18130.15452683508074516No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT16260.1385883253399292No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT15680.13364483034010394No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC15480.13194017689188833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.12622958784036603No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14670.12503633042661508Clontech Universal Primer Mix Long (96% over 26bp)
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG13330.1136151523235705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)