FastQCFastQC Report
Tue 10 May 2022
EGAF00003657520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173259
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC24300.20711539395819678No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG22180.1890460674071113No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC21540.18359117637282135No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19110.16287963697700167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC19100.16279440430459088No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT18970.16168637956325074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18850.16066358749432136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16600.14148623620189574No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT16050.13679843921930282No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14910.12708191456447382Clontech Universal Primer Mix Long (96% over 26bp)
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT14410.12282028094393481No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG13440.11455271172008909No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA12260.10449525637561698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)