FastQCFastQC Report
Tue 10 May 2022
EGAF00003657613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398500
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG36060.9048933500627352No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT35310.8860727728983688No Hit
GTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC35020.8787954830614805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29610.7430363864491845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21740.5455457967377666Clontech Universal Primer Mix Long (96% over 26bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT20190.5066499372647428No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT19600.4918444165621079No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA18510.46449184441656216No Hit
GTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGC15030.37716436637390216No Hit
GAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT12760.3202007528230866No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC11770.29535759096612296No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC10820.2715181932245922No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT10770.27026348808030115No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10690.26825595984943534No Hit
GTACATGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG10670.26775407779171895No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG10280.2579673776662484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.2409033877038896No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT9390.23563362609786703No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9340.23437892095357593Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCC9270.23262233375156838No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT8770.22007528230865747No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC8230.2065244667503137No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT8060.20225846925972396No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA7980.2002509410288582No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC7810.19598494353826854No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7480.1877038895859473Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7200.18067754077791717Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCT7060.17716436637390212No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG7020.17616060225846925No Hit
CATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC6820.1711417816813049No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6580.16511919698870764Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATC6570.16486825595984944No Hit
ACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCT6420.16110414052697616No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG6240.1565872020075282No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT6230.15633626097867No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6080.15257214554579673Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC5930.14880803011292346No Hit
GAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA5850.1468005018820577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.14554579673776663No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG5740.1440401505646173No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5670.14228356336260978Clontech Universal Primer Mix Long (96% over 26bp)
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT5500.13801756587202008No Hit
ACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT5220.13099121706398997No Hit
GGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTG5180.1299874529485571No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5120.12848180677540777Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5100.12797992471769135No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG5050.12672521957340024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT5030.12622333751568382No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA4970.12471769134253452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA4900.12296110414052698No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG4620.11593475533249685No Hit
TATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTG4540.11392722710163111No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG4510.11317440401505646No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT4470.11217063989962359No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC4440.11141781681304894No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC4420.11091593475533251No Hit
ACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA4410.11066499372647427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAA4400.11041405269761606No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT4330.10865746549560854No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG4240.10639899623588456No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC4220.10589711417816813No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4150.10414052697616061No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT4110.10313676286072773No Hit
GTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAACGTCTGCC4080.10238393977415308No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG4070.10213299874529484No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4070.10213299874529484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)