FastQCFastQC Report
Tue 10 May 2022
EGAF00003657659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324269
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA287528.86671251337624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT183115.646854926002794Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG103733.1988873435326846Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG94392.910854876661044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT67702.0877728059111416Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63761.9662687460102568Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC57471.7722939904832098Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC51401.5851037256105271Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50971.5718431302406335Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40741.256364314812702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG29750.9174481680333304Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG25980.801186669092636Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT25460.78515060027323Clontech Universal Primer Mix Long (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC23420.7222398687509444Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG23160.7142218343412413Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22510.6941767483169837Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA22120.6821496967024291Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22060.6802993810694208Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20710.6386672793267318Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20190.6226312105073257Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20010.6170802636083005Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC19810.6109125448316058Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC19080.5884003712966703Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16320.5032858521782841Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT14880.4588782769860826Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14740.4545608738423963No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG14590.44993508475987526Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14020.4323570862462955Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13540.41755456118222833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC13530.4172461752433936Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA13230.40799459707835156Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12360.3811650203997298Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10450.32226330608229586Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.30437692162988134No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9090.28032281840077217Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8680.26767899490854813Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8530.26305320582602715Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8520.26274481988719245Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8510.2624364339483577Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTCTACTGATCTCGTATG7260.22388819159401607No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG7080.21833724469499088Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7010.21617854312314774No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6310.19459152740471647Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1911992820775344No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT6090.18780703675035235Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5970.18410640548433554Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5900.1819477039124924Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA5830.1797890023406493Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG5770.17793868670764088Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA5750.17732191482997142Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTCTACTGATCGCGTATG5730.17670514295230194No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT5430.16745356478725995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5140.1585103725610527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTCTACTGATCTCGTATGCCG5060.15604328505037485No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC4950.15265103972319277Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.15234265378435805No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG4810.14833363657950652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4680.144324619374655Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG4620.1424743037416466Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4610.14216591780281185No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4450.13723174278145614Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT4420.13630658496495196Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4400.13568981308728248No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA4360.13445626933194355Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT4240.13075563806592674Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4050.12489630522806681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTCTACTGATCGCGTATGCCG3810.11749504269603323No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3810.11749504269603323Clontech SMART CDS Primer II A (100% over 26bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.1144111833076859No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3670.11317763955234698Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3510.10824346453099125Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3450.10639314889798286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3390.10454283326497445Clontech Universal Primer Mix Long (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCCGTTTCTACTGATCTCGTATGCCGTC3370.10392606138730498No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.10145897387662713No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3250.10022543012128818Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)