FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCATATATTCTTCCTATACATGAGCATGAAATGTTTTCCCATTTGTTT21.5384615384615385No Hit
ATACAGCCTTAGATTTTACTTCCTACTTTTGCAGCCAGAAGTCAGTGCCT21.5384615384615385No Hit
CTCCAGGGCCCGGGCCCTGTCCCCCACCCCAGCATGGTAAGAAGGCAGGG21.5384615384615385No Hit
CATCTAATGCTGATATTCGATATGTGCGTGATGGAAACATTGTTACTGGG21.5384615384615385No Hit
GAGTAGAATGGAATGGAATGGAATGGAAGGGAATGGAATGGAATTGAATT21.5384615384615385No Hit
GTATTAATCCTACTTTAAGGAAAAGAAGGCTCCCAGATGAGCTATGAATG21.5384615384615385No Hit
CACTGCTCACTGTGTGTTCTTTCTGCAGAGACGACACCCATAAGGTGGAT21.5384615384615385No Hit
GGGCAGGAGGCTGCCCTTCAAGGAAAGAACAACCTTATTTGACCTTCTTC21.5384615384615385No Hit
CGCGTGCACCTCGCGCTGCAAAGCAGCTGGAACGGCCGCAAGGTCGGCAT21.5384615384615385No Hit
GATCACCGGCGACGCCGCGGTGCACGCGCTCTTCCTCGCACGGGTACGGG21.5384615384615385No Hit
GTCCAGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGG21.5384615384615385No Hit
GATTACAGGCATGAGCCACTGTGCCCAGCCGCCAATTGCTATCTTTTTAT21.5384615384615385No Hit
AGCCAGGATGGTCTCGATCTCCTTATCTCATGATCCACCCGCCTTCGCTT21.5384615384615385No Hit
GCGGTAGACGCCTTCGGCGTTGCGCACGTCGAGCGTCACGCCTTCGTCGT21.5384615384615385No Hit
CACCAAGAATGAGTACACCTGTTACAGCTGCAAGAGAAGTAAGAATTGAG21.5384615384615385No Hit
CTACTAAAAATACAAAATTAGCTGGGCATGGTGGCACATGCCTATAATCC10.7692307692307693No Hit
GTAATTATTTATACAACTGTGATTCTACTTCTTATTCTCTTTTTTCATAC10.7692307692307693No Hit
GCAATACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCA10.7692307692307693No Hit
TCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGG10.7692307692307693No Hit
ATTCAGAACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGG10.7692307692307693No Hit
TCATGAACTTGATGTTTGTAAAAGTCATACCCAAGGAAAGGGCTACTAAT10.7692307692307693No Hit
GTAAAATTAAGTACACAGACAAACCTGGGATACTCTAATACTGGAATTGT10.7692307692307693No Hit
GCTGTGATCACACCACTGCAGTCCAGCCTGGCAACAGAGAGAGACCCTGT10.7692307692307693No Hit
GGATATTTATGCATGTAAATTTAATATCTTAAATAAAATGAACTGATTCC10.7692307692307693No Hit
GCCAAGCACATACACCAAATGTCTGAACCTGAGGTTCCTCTCGTACTGAG10.7692307692307693No Hit
GACCTATTAAGAAAAAAAGGGAGAAAATATACATTTTCAGTGTCAGGAAT10.7692307692307693No Hit
GTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAA10.7692307692307693No Hit
GTAATGTATGTCCTTTAGGAAATAAAAAATTCAGTGATGATGGACTAATA10.7692307692307693No Hit
TGTTTAATCTGGTTCATGGGTATTTGTGTCTGTTTTTATTCCAGTACCAT10.7692307692307693No Hit
GATCTACACTTTTTTTTTCGTCGGCAGCGTCAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GACACACGGAGATTTTGGGAACTACAATTCAAAATGAGATTTAGGTGGGG10.7692307692307693No Hit
GTACCAATTCTATGTCTTGTGAATATCTTCTCCCAGTATACGATTTGTTT10.7692307692307693No Hit
AGCGGAGACGGGGAACGGGGCTGGGCACGCGAGGATCTGTATGATGCCCA10.7692307692307693No Hit
GTTACCAGTAGTTCCTTTCCGTGTTTAGTGCTTCCTTCAGGAGCTCTTGT10.7692307692307693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.7692307692307693No Hit
TATGAGTTGTGTGACTGGACCAGAGATTGTGTACTCTGGAAAACACAATG10.7692307692307693No Hit
GCATACGCTGGTGGCCGGCCGCGTCGTCCACGAATAGAAATGAAGCGCAC10.7692307692307693No Hit
CCGCCAGTCCGTCGACTTTCTCGACCGGCTGCTGACCGGGATGCTGGACC10.7692307692307693No Hit
ACCCCATCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGCGTGTG10.7692307692307693No Hit
CTGTCACAATTTTAAGGTTCCCCTTCCCCTCTTAAAGGGACAGAAGTCTT10.7692307692307693No Hit
GGACAACATGTTTGTCTCCAAGATTCCTTTCACAGGGAAGCCAAGCCAGA10.7692307692307693No Hit
GCCAAACCGGGGGGCAGGGACTGCTTCAGTTTCTCTGCCTTCTCTTTGTC10.7692307692307693No Hit
ATATCCAATTGGGTTTCTACTGTCATTGTCCCAATCTTATTATTTCCTTT10.7692307692307693No Hit
CTTCTATTTGTTCCATCATTTTTTGCTCCTCTGTTCTTTCTTTCATGCCT10.7692307692307693No Hit
GTTTGTATTCAGGACACAGAGTTGAACATTCCCTATCATAGAGCAGGTTT10.7692307692307693No Hit
GAAGTGGGGAAGGTAGGACTGGGCAGAGGCAGAAGCTGACCTGCAATGCA10.7692307692307693No Hit
GAATGAGAAAATCCTTTCCTTAATTTTGACCCCAGGGAGCTGGATGATTT10.7692307692307693No Hit
CTAACTAGTTACGCGACCCCTGAGCGGTCGGCGTCCCCCAACTTCTTAGA10.7692307692307693No Hit
GATTAATGAAGACACAGTCTTCTGAGTGGGATTCACAGAAACAGACATCC10.7692307692307693No Hit
TCATGGATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGC10.7692307692307693No Hit
ATTTATTCCAGGAATGCATGGTTGTATATAATTTGAAAATTGATTAATGT10.7692307692307693No Hit
ACACTTGACTATGCTGAGTATAGACAAGTTATAAGAAAACTTTGACCAAA10.7692307692307693No Hit
GCATGTCACTGCTGTTCCCAGCATGTGCTTTCTTGGGCATTGTCCAGGGT10.7692307692307693No Hit
AGCCAGTTGTGGCCTAAGCACATGTGCCAGCTGGATGGTGATACTTAACT10.7692307692307693No Hit
GTTTCCTAACTGCTCTATGAAAAGAAAGGTTAAACTCTGTGAGTTGAACG10.7692307692307693No Hit
AGTATAAACAACTGAGTAAATAAGGCCATTACTTTCTAGATTTGAATGCG10.7692307692307693No Hit
ACTCTGAGAGTTGAACGCACACATCGCAGAGCAGTTTCTGAGAATGATTC10.7692307692307693No Hit
GATTAAGAGTCTACAACCTTTCAAAGATCTGAATAGGAAACACTTGTCTG10.7692307692307693No Hit
AGTGACTACTGTGTTGAACAGCACCCTATAAGGCTTCGTTCCCCCATGGT10.7692307692307693No Hit
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC10.7692307692307693No Hit
GCATGAATTGCACAGATATGTTAATGAAAATAAGTTAAAACCTTAAGTAA10.7692307692307693No Hit
AAATACATGTGAGCTCCTTATGACATTAACCAGAACCTTATGATCCATTC10.7692307692307693No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC10.7692307692307693No Hit
GAAGAGAAGGAAAGAAAGAAAGAAAAAGAAAGAAAGAAGAAAGAAAGAAA10.7692307692307693No Hit
CTTAGTATGTATTTTGACATATTTTATAAATCCCACTCATCCTTCATGCC10.7692307692307693No Hit
GTTCTGGACCAGGTGTGGTGGCTCATGCCTGTAATCCCAGAATTTTGGGA10.7692307692307693No Hit
GTGCTGGGTTTTACTATGCAGACTCAGTATTGGGGTGCAAGGCAAAGTCC10.7692307692307693No Hit
GTGGAGCTAATAAGCATCATGACTTCTCATCTCTTTTTAAAAGTCAGTCT10.7692307692307693No Hit
CATGCCAACACACCCAGCCAATTTTTATATTTTTTTGTAGGTAAGGGATT10.7692307692307693No Hit
ACATCTTTCAGTCAAGAAACAAAAGTTTATAAACATTTGATTGTTAATTC10.7692307692307693No Hit
GAATATCATCTCCTACCGGAAGACCCAAGGAAATCCGGAATTTCGCACCA10.7692307692307693No Hit
TTTCTAAGCTGCCTGAGGCACAGGGAACTGAAAAATTGTTTCCTTCTCTC10.7692307692307693No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAG10.7692307692307693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAA10.7692307692307693No Hit
TCGTGGAAGCCCGCGTCGAACACGCTGATGGCCGGCGGCGGCGCCTCGAT10.7692307692307693No Hit
CGTAGGTAGAAAAAAATTGGCAAGTGACTGGTTCTTTTAAATAGCTCTTA10.7692307692307693No Hit
CCATATGTACATAGAAAACCTGACAAATAATAACAAAAACAAAAGCTAAC10.7692307692307693No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTCTGTCTCTTATACAC10.7692307692307693Illumina PCR Primer Index 5 (96% over 30bp)
ATTCGATTGCATTCGATAATTCCATTCGATTCCATTTGAGGATAATTCCA10.7692307692307693No Hit
TGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACA10.7692307692307693No Hit
TCTGGCCAGGTGTGACTTTTAGAGAGTTAAGTAACTGACCCCAGCAGCTA10.7692307692307693No Hit
GGCAATACACGAACCGATAGCCGTCGATCTGCGCGACGGTGGCGTCCATC10.7692307692307693No Hit
CATTATCCAATATCAACGAAATGCTTGGCCATAAAGCAAGCCTCAATTAA10.7692307692307693No Hit
GGTGCTATCTCAGCTCGCTGCTACCTCCACCCACTGGACTTAAGCGATCC10.7692307692307693No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT10.7692307692307693No Hit
CATTGTGGTTCTGATTTGCATTTCTCTGATGACTAGTTGGTGTCAGTTAT10.7692307692307693No Hit
GAGGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGA10.7692307692307693No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
ATTTTGAGGTCGCTATGCCTGCAAAGAAAAACGAATTGGTAGTGAAGTCG10.7692307692307693No Hit
AAATAAGCCATAACCAAAATGGAGGTCATATTTGTAGATAAAAGCACACA10.7692307692307693No Hit
AAAATACAAAAAGTAACCACGCATTGTAGTGAGCACCTGTAATCCAGCTA10.7692307692307693No Hit
CCCTGGATGAGGGGTTCCTCCATGGGGTCCTCCTGGGGGTCGAGGTGGTC10.7692307692307693No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAA10.7692307692307693No Hit
GTTCAAGACCAGCCTGGCCAACATGGTGAAACTCTGTCTCTACTGAAAAT10.7692307692307693No Hit
ATGGTTGACGGTATTTCGCTGCCAGAACTGCTGGAAAAACGTACCGGCAA10.7692307692307693No Hit
CCCGTAAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGCAC10.7692307692307693No Hit
GAGTCCAAGTTCTTCTAGTCTCGCTTTCACTTCTTCAAGTGATTTGCGTC10.7692307692307693No Hit
CCTTTCTTCAGCAAACAGTCTGTGGGCTGCATCCACTGTCTGACAAGCCC10.7692307692307693No Hit
CATCTGTACATTACAAAATTATATTTCTCTGTGGAACAGCAGCAGTAACC10.7692307692307693No Hit
CGGCCCCACAGACCCAGCACGTATCTCCCATGCGCCAAGTGGAGCCCCCA10.7692307692307693No Hit
ATCTACATGCCGCGCTGGGCCGGCACCATCTACGAGGTGCTGTCCGCACC10.7692307692307693No Hit
GTTCATGCTTGAAGTTTTAATGACAAAGAATGAAAACAATAGCCATGCCT10.7692307692307693No Hit
CTCCCGAGTAGCTGGGATTACAGGCGCACGCCACCACACCCAGCTAATTT10.7692307692307693No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG10.7692307692307693No Hit
CAGCAACAGCGTGCGGTGGCAGTGGCGCGGGTCGCGTTCAAAACAGAGCA10.7692307692307693No Hit
CTCATGAACAAATTCTGTGTGTGTAAAGTACTACAATGTACCTATTTTAG10.7692307692307693No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG10.7692307692307693No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10.7692307692307693No Hit
CATCAAAGCCTTTTACGCCGCTGCCGGCGGTTGTTCTTGTTGGCGATTGC10.7692307692307693No Hit
GCAATTTACTGAGTGCCTGTATCTTACTGTGACAGTCGTTGTGCACATGT10.7692307692307693No Hit
ACCTGGTGCTGACAGTGCTCATTGCCCTGGCCGTGTACTTCCTGGGCCGG10.7692307692307693No Hit
GCATTTGGGTTGGTTCCAAGTCTTTGCTCTTGTGAATAGTGCTGCAATAA10.7692307692307693No Hit
GCTTTGAAGTCAAAGGTAGAAAAGGAAATATCTTCCTATAAAAACTAGAC10.7692307692307693No Hit
CTTTTAACTTGCATTGGTTTGTTTGAGGCTTGAATTTGTGATGTTTTTCC10.7692307692307693No Hit
GAGCAAACAAATCCCAAAGCTAGCAGGAGACAAGAAATAACTAAGATCAG10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)