FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAGATCACAGCTTCTTTGGGACACTTGCTGCTGGTGATTCTTCTAT47.8431372549019605No Hit
GCATTGAATGCACTCGAAAAGAAGGAACATCTAAAATCAATAATCAAAGC35.88235294117647No Hit
CCGAACCCGGCCGCTGGATCGCCGGCCCCGCCGGGGTGCTCCTGTCCCGC35.88235294117647No Hit
AGCCAGTGGAGGCGACGATTCGGAGAGAAGTGTCGACATGACGACGGATC23.9215686274509802No Hit
CGATTGGAGACTTCAATACCCCATTTTCAATACTGAACAAAACATCCAGA23.9215686274509802No Hit
CCCGTTGGAATGTAAACGATATCCAAAGCCGCATCATAGGCTAATGGTGC23.9215686274509802No Hit
GTCTCCCACCCTCCAATATATGCATAAGAGATTTTCCTGTGAAGAAAGCA23.9215686274509802No Hit
GAAAAAAAGGTTCCTTGCCCAGTTTCCCAACCAGTCAGTAACAGGACCGG23.9215686274509802No Hit
GAATGTACATGAATGGAATGTAATCGAAAGGAAAGTAATCCAATGGAATA11.9607843137254901No Hit
GAACCTCACTCAACCGGCCCGGCGCGTGGTGGCGCTCGAATACTCGTTCG11.9607843137254901No Hit
CGAATGGAATGGACTGGAATGGAATGGATTGAACGGAATGGACTGGAGAG11.9607843137254901No Hit
CAACTATCCACTTGCAGATTCTATAAAAAGAGTGTTTCCAACGTGCTGTA11.9607843137254901No Hit
CCTCGATGGCGAGTGGCGCGCGCGACAACTCGGCCAGCTCGAACAGCTTG11.9607843137254901No Hit
GTGCCCTGCAAGCGATTCAATTCCCCAAGCAACTGGAAGATGCTGTCGGC11.9607843137254901No Hit
CATGCCTAGATCTCAGACTTATAACCTCCACAACAGTGAGACAATAGATT11.9607843137254901No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAGAACTGATAAGCACTTACTCAGATGAGCTGAAACAACTAGACGTC11.9607843137254901No Hit
ATGGTGGGCACCTGCGTCGCGGCGGCGGTCAGCCTGTGGCTGCCGCTGAT11.9607843137254901No Hit
CGTGAATGCTGCCGTGGAGCAAGCATCCGCGATTCAGGAGGCCCTGGGGC11.9607843137254901No Hit
GCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAA11.9607843137254901No Hit
CTTCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ATTGAATGTCGCGGCGGCGGCATTCAGATTCGCCGAGCCACTCCAGACGC11.9607843137254901No Hit
GTCGCTGTAACTCAGTCTCACCTAACTCTCCTGGAAGTCCCCACGATAAG11.9607843137254901No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GACTAACCCAGAAACATCCAATTCTCAAACTGAAGCTCGCACTCTCGCCT11.9607843137254901No Hit
GCTGCTGCCCTCCCGGGCCCGTCGCCACTACTCGCGAGGCGCCATGCTGC11.9607843137254901No Hit
ATTCCTAAATGTTACTCACCCGTCCGCCACTAATCTTCTCTAGCAAGCTA11.9607843137254901No Hit
CCACCCGCCTGCGCATCGACGCGCCGGCCTATACCGGCCAGGCCACGCTT11.9607843137254901No Hit
TGCGGGATGCCTGCAGCCGTTCACGCTCCTCGACCGCCGCGACAGTCTCC11.9607843137254901No Hit
CTGTTACTCTGTTCTTGATTTACTCTCCCAATTAAAGAGCATTTTTATTC11.9607843137254901No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA11.9607843137254901No Hit
CTCGTCATCAACAAGATGGATATCCTCGCGGGCCTGGAGACGGTCAAGGT11.9607843137254901No Hit
CATGCAGCGCGTCATAGAGCGGGCGGAGATAGGGGTCCACCTTCTCCTTC11.9607843137254901No Hit
AAATTAAGAACATGTAAGAACAGGGTGATTTAGCAAGAAGTAGGTACATG11.9607843137254901No Hit
GTTGTCGAGGTTGCCGCCATGGGCGCGCGGCTCCTTGGTGTTGATGCGCC11.9607843137254901No Hit
ATCATTTTCGGCGCATCGTCACTCGACCAGTGAGCTATTACGCACTCTTT11.9607843137254901No Hit
GAGCTTCCGCTGCAACTTGAGACCACATGCGGCAAGTCTCTTGCAAAATA11.9607843137254901No Hit
CTTTTGTCTGAGCCTGAGGGCTTCTCCAAAGGTTATTATCTGCATTAGAT11.9607843137254901No Hit
GTTTTAGTCATTGAGGGGGAAGGAAAAGGAATAGCAAGGAATCCATTTTT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)