FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGAAGCGGCGGGTGGCGCCGCGCTTGACCAGCATGACGCGGGACAGG35.88235294117647No Hit
ATATTCACACAATAATAAAAGTGCCTAATGATGTATTTCTCAGAATGTAT35.88235294117647No Hit
GAGTACATGGGGAGAGGCTGAGACTAACCCAGAAACATCCAATTCTCAAA35.88235294117647No Hit
ATAGAAGACTCATCAGAAGCAATGCAATGGAAACAGCATGAGACCAGGAA23.9215686274509802No Hit
CCAGGAGAGAACACAAGATAGGTAACAAAACAAGCCTTAGCAAATTTAAG23.9215686274509802No Hit
ATGCGTCGCCGAGTGTGTTTGCCCCCAATCTGCACACCTGGAATCGATCG23.9215686274509802No Hit
GATACTGGTGAGCGTTGTACTGACTTTGGTAAAAATGGTGAAGTCGATTT23.9215686274509802No Hit
GTCCAAAGACTATGCCAGGGTCAGGAATTTGGTTATAGAAACTCAAGGTG23.9215686274509802No Hit
GACCTATCTCGCCGTCGCCCAGGGCGTCGCCCTGCTGATGGCGGGGCGGG11.9607843137254901No Hit
GTATCTGGCTGAGCGAGCCCTTGGGGAATGAAGGTGGCTGCTATGAGCAG11.9607843137254901No Hit
GGATAAGTACATCCTAGAAATTTCTCATTTATTTTCCTCTTAACACATTT11.9607843137254901No Hit
GAGTACATGGGGAGAGGCTGAGACTAACACAGAAACATCCAATTCTCAAA11.9607843137254901No Hit
CCATTCCTTTCGTTTCCATTCCATTTGATGCCATTCCATTCAATTCTGTT11.9607843137254901No Hit
TGGTGAAACCCCGTCTCTACCAAAAATACAAAAATTAGCCGGACGTGGTG11.9607843137254901No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCGTAGGTCTGCTCGCGGGCCGGGCCGCACGAGAAGATGACCACCGGGCA11.9607843137254901No Hit
ATTCCATTCTATTGGGTTGCATTACATTCGTGTTCATTCCATTCCAGACC11.9607843137254901No Hit
TACAAGATATGTTGCTTTTGATCCTGTCTAGGAGAATTTGTCTTTCAACA11.9607843137254901No Hit
GCGGTATCCGGGCTGACGCGCCAGACCGTCATCGCCCGCACCGGCATCGA11.9607843137254901No Hit
GTTTTACAAGAGGAAATTGTTTCCAACTTGGAGTGTTTTATCAAATACTC11.9607843137254901No Hit
GTATTATTTAAAATTAGGTGTACCTCTTCTAAAAGAACAGGATATTCCCC11.9607843137254901No Hit
TCCTTGATGAGTCATAAATTCTAAATACATTTTTCAATGCCTATAGTTGA11.9607843137254901No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGTGCGTTTTTAACACG11.9607843137254901No Hit
GGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTC11.9607843137254901No Hit
TAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACA11.9607843137254901No Hit
ACCTTGTTTGGTGTCTCATTTTATTGTTTGCTTTTCTGCAACTTATGATG11.9607843137254901No Hit
CTGGAGCATCGTGCGAATCACCGTCCATCTGAACTTTCTGGCGGCGAACG11.9607843137254901No Hit
ACACCGCGTAGCGATGGCGATAGATAGAAAGATTAAACTGAAGAGTTTGA11.9607843137254901No Hit
GGTCGTCGAGGCTGCCCCGGCGGCTCTGGAAAGCGGCGGTCGGGGCCAGC11.9607843137254901No Hit
ACCTATCTTTTGATACAGCACGTTGGAAACACTCTTTTTATAGAATCTGC11.9607843137254901No Hit
GTTGGGCGCATGGCCGGCGCAAGCTGTTCGAGCTGGCCGAGTTGTCGCGC11.9607843137254901No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCAGGGCCTCCTGAATCGCGGATGCTTGCTCCACGGCAGCATTCACGC11.9607843137254901No Hit
GACGCGATCAGGAAGCCCGCGGGGATGCCCTGCCGCAGGATATCGAGCGG11.9607843137254901No Hit
GTCTCTGCCAGTGCCGGTTCGCTCACCACGCGGTACAGCACCGGCGCGCG11.9607843137254901No Hit
TCTTTGCAGCATAAATACAAAAACATTTAATTTAAGCTGATTTCCTTTAC11.9607843137254901No Hit
CGGCTGCACCACGCGGATCGTGTCGGCGGCGTTCTGCGCGATGCGGCGGA11.9607843137254901No Hit
ATCTAGACTCAGTGGTTAAGGATGTGTCGTCGCACAGACAGCCAGGAGGT11.9607843137254901No Hit
ACATCGAGCGCATCGAGCTCGGCGCCGACTGGGGCTTCTGCGGCTTCCGT11.9607843137254901No Hit
GGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGT11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)