FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGAGAGCTATCTGTGGTTTAAATACAACCATCAACTTCCCCCAGC36.666666666666667No Hit
CCACAACACCCGATTAATTTTTATTTTTATTTATTTTTTTGAGATGGAGT24.444444444444445No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT24.444444444444445No Hit
GCCCTGGGCCACCTTGTTACACACAAGAGGGCCTCCAGAGTCCCCCTTAA24.444444444444445No Hit
TGGTCATTGATGTCCTTCACCCCGGGAAGGCGACAGTGCCTAAGACAGAA24.444444444444445No Hit
GGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAATTAGC24.444444444444445No Hit
GGAGAGGGGACTACATTTAGAGAGGAGGGAGGAGAAGTGGCCTGCATTGG24.444444444444445No Hit
CTCTGTTACAGCTCTGACCACGAAAAACTGAAGCCTCAGTACTTGGAAGA24.444444444444445No Hit
GAAGTACAAAGCAAAAGCAGCAGAGGCATGTCAGTCCAGATAAAATATTG12.2222222222222223No Hit
TGCCTAGGCCGGTCTCTAATTCCTGGACTCAGGAGATCCTCTCACCTCAG12.2222222222222223No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT12.2222222222222223No Hit
GTCCTGGCTGACCAACGCGAAAGAGACGAACACGGGGTTGTAGGAAACGT12.2222222222222223No Hit
TAGTAATTCCTGGTAAAGGGCCCGAACAGCTTCACCACGGCGGTCATGTG12.2222222222222223No Hit
ACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTA12.2222222222222223No Hit
CCATAGAACGTTCGGCCTTCCCCCTTCGCGTGGAAGACCGCCAGGAGATC12.2222222222222223No Hit
TCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAG12.2222222222222223No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA12.2222222222222223No Hit
ATCCCATTCCATTCCATTCCAATCCGTTGCATTCCATTCCATTACATTCG12.2222222222222223No Hit
ACCGCGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACAT12.2222222222222223No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG12.2222222222222223No Hit
GTACATGGGGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC12.2222222222222223No Hit
GTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCA12.2222222222222223No Hit
GTTGTGTGCTGATTTCCACAAAACATAATATAAGAGACTCTTCACAAATA12.2222222222222223No Hit
TCTAAATTCTTACATTTCCATTCTACATTGAAATACAAAAGGCCAAAACC12.2222222222222223No Hit
GTCCACACTGGTAGCCAACCATGAGGCTGTACTCGCAGCTCTGGCCCTGG12.2222222222222223No Hit
TGCTAGTAGCTGCCTTCTGCTCTCCAGCGCTCTCAGCACCAATGGGCTCA12.2222222222222223No Hit
CATCAAATGGAATCGAAAGGAATCATTGAATGGAATCGAATGGAATCATC12.2222222222222223No Hit
TTTCCGGGCACTACAACAATATACGGACAACCGATCTCGCCAGCGATCCT12.2222222222222223No Hit
GAGTTGAGGTGCGAGTTACACCAACCAGGACAGAAATCATTATCTTAGCC12.2222222222222223No Hit
CGGCTCTGTTGGGCGAGCTAGTCTCAACACGGGCACACTCCTCCTTCTCT12.2222222222222223No Hit
TCGAGTGGAATCGAATGGAATCATGATCAAATGGAATCGAATGTAATCAT12.2222222222222223No Hit
GTACTGCACCAAGTGCTCCAAACAGTGGTTCAAGAATCTGTTGTTCCAGT12.2222222222222223No Hit
GCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCC12.2222222222222223No Hit
GGGTCTCTAGCTTACCAACTGCAAATCTCAGGACTTGTTAGCTTCCATAA12.2222222222222223No Hit
GATGCAGGAGTGGATGACTTCATTGCTCGGGACTTTGTTGCTTGGGTGAC12.2222222222222223No Hit
GGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCT12.2222222222222223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)