FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAGGAAATAACAGAACTCCACCTCTTTGCACACATACAGTGATCAT36.666666666666667No Hit
GATCACACCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTGTCTAAAA24.444444444444445No Hit
ACTGATCAGACTGTAAGTAGGCAGCGATTTTCTCCAAAGCCTCAAAACGG24.444444444444445No Hit
CAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAAT24.444444444444445No Hit
GACCAGTACTGTGGGGACTATGAGCTCTTCGTGGAGGCTGTGGAACAAAA24.444444444444445No Hit
CTGCACTCCAGCCTGAGCAACAGAGTGAGACTCCATCTCAAAAAAATAAA24.444444444444445No Hit
AGTTTATCCCTGTAAAAAGACCCATCCCCCATCCAGCCTATAATCCTAAG24.444444444444445No Hit
ATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTC24.444444444444445No Hit
GGACCATCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATA12.2222222222222223No Hit
CCTCCATACGGAACTTTCTCATCTACGTGGCCCTCCTGCGAGTCACTCCA12.2222222222222223No Hit
GTTCAAATAAGAAGTTGCAGTATAACTTGGCTGAGGAGTCAGAATTTGGA12.2222222222222223No Hit
GGCAATCCGGTCTACCAGCACCTGCCGCCCCATGCGACCGTCAATCGTGC12.2222222222222223No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC12.2222222222222223No Hit
CCCAAAATCCCAGCACTTTGGGAGGCTGAGGTGAGAGGATCTCCTGAGTC12.2222222222222223No Hit
GTCACCATGGTAGGCACGGCGACTACCATGGTGACCACGGGTGACGGGGA12.2222222222222223No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA12.2222222222222223No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12.2222222222222223No Hit
ATCTACACCCGCGTGGCGAGCTATGCGGCCTGGATCGACAGCGTCCTGGC12.2222222222222223No Hit
TCCATTTGATGTTGATTCCATTCGATTCCATTTTATGATGATTCCATGCA12.2222222222222223No Hit
GGAATGGAATGCAATGGAATGCAATCAACCCGAGTACAATGGAATGGAAT12.2222222222222223No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT12.2222222222222223No Hit
GATATAGGCGAAAGAAGCGCCGCCGGCCAGGCCGCGAAGCCGGTCTGTCG12.2222222222222223No Hit
GTTTTAATATTTTCTTTTGTAGCCTTGACACTGATGGACATTTTCCAAGC12.2222222222222223No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC12.2222222222222223No Hit
ATGTAGAAATCAGAATGTAGAATGTAAAATGGAAAAAGAGTAGCTCCGAT12.2222222222222223No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT12.2222222222222223No Hit
GTTTCACTATGCTGTTGGCCATGTGGCTAGTGTGTGGATCAGAACCCCAC12.2222222222222223No Hit
GAGCTGCACTGTGCCACGGGCATCAGCCTGGCCAACGAGCAGGACCGCTG12.2222222222222223No Hit
GATAATTCCATTCGATTCTATTCGGTGATTCCATTCGACTTCATTTGATA12.2222222222222223No Hit
AAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCT12.2222222222222223No Hit
GTGGATCATCAGGCCATCCACAAACTTCATGGATTTAGCCCTCTGTCCTC12.2222222222222223No Hit
GGGTTTCACGGTGTTCGCCAGGATGGATACATTTTATAATATATGGGTGA12.2222222222222223No Hit
GGTCCACCACTGACACGTTGGCAGTGGGGACACGGAAGGCCATGCCAGTG12.2222222222222223No Hit
GTCCCAGCCGGGTTCGTGTCGCCATGGGGCAGATCGAGTGGGCCATGTGG12.2222222222222223No Hit
CCATTCACTCCAATGCTGAGCACTTCCACAGAGTGGGTTAAAGCGGCTCC12.2222222222222223No Hit
NCACATGTTTGTGGTTAAGATAGAAGCCATAAACTATCCTTGTGGTCCGA12.2222222222222223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)