FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAAGCGTTCCGCCGACGCGCGGGCCGTGCTGGTGGCGCTGAGCCAT34.918032786885246No Hit
TCCCTGGTTCTGGGACAGCAGGGTCTCAAAAGGCTTCAGTTGCCCGGGCA34.918032786885246No Hit
CTATGTGAAAGAATCTCCTGATGTCATAATTTCCGGGTGTCACCGGAACA23.278688524590164No Hit
GCTGAATGTTCGGCGGCCATGGGTCTTCGGATCCATGGCCGTTTGCATGT23.278688524590164No Hit
CGACGAACGCCCCCGTCGCGCCGGGGTCAGCTCCCTGGGCGTGGGCGGCA23.278688524590164No Hit
ACCGCATCCTGCGTCGCGCCGAAGTCGAGGCCAAAACCGGCTTCAAGCGC23.278688524590164No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT23.278688524590164Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTAAAGAGTTTGTAGAGGCAGTTCGGGTGCGGTACGTTGCATTCCGG23.278688524590164No Hit
GTTAAATTATAGGCTAAATCCTATATATCTTAATGGCACATGCAGCGCAA23.278688524590164No Hit
CTGCAGAATTTTGCCCGCAAATTTGTCATCTCCATTGACACCATCTCCTT23.278688524590164No Hit
TCCTTGCGCCTGTCCGACGCGGACGCCGGGCCGTCGATCACGAGATCGAG11.639344262295082No Hit
ACCCTGGCGAAGGAAGCCGCCCGGACCGACCGAAACGACGCGCGGACGGT11.639344262295082No Hit
CCCAGGAACTTGTCCGGTACGGCCACGAGCGCCACGTCGGCGACATCGGG11.639344262295082No Hit
TATCTGAGGAAAGAGGATTCCAGACAGAGAAAATGGCCAAGAGCATATCT11.639344262295082No Hit
TCTTTCAGGCCTCCAGCAGCTTTCTGGCGCCTTCGAGGTCCGGCATGAAG11.639344262295082No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GGTGCTGGGCCCGGAGTTCGTCATCGGCCAGAGCCTGGCCACCGACGATG11.639344262295082No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
ATTTGACCCTATGTATCCCACTGTAGGCTCAAACACTGCACAGAGCTGCC11.639344262295082No Hit
CGTCCGGGCCTCCCATGTGCTCCGCAACCCTCCGAAGCGATGACCTGGCC11.639344262295082No Hit
GACCTCGACGAGCTCAAGGCCATCAACGACCGGCTCGGTCACTTCCACGG11.639344262295082No Hit
GTCTTGAAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTCCTTACA11.639344262295082No Hit
GGACACTTGCTGCTGGTGATTCTTCTATAGCTCGCGAGCCTCTGCACTGA11.639344262295082No Hit
CTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTC11.639344262295082No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA11.639344262295082No Hit
ACTGGGGGAAGCCTCGCCGCGAGCCATGGCGCGATCGATCATGACCTGCA11.639344262295082No Hit
TACCACTGCTTCCCCATGTACTCTGCGTTGGTATCAACGCAGAGTACATG11.639344262295082No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA11.639344262295082No Hit
GTGCTCGTGGCCGCGGTCAGCCCATTCGCCGGCAAGCTCGGCGACTGTCT11.639344262295082No Hit
CCCTTGCGGGGAATATCCAACTCGTGGTCTAAGGTAGGGAGGAACCGAAT11.639344262295082No Hit
GGCTGGGCCTAGCCCTGTTGGGGGCTCTGCATGCCCAGGCCCAGGACTCC11.639344262295082No Hit
AAGGTCGTGCGCGTGGTTCTCGCAGGCATCGGCGAAGCCTCGGGCAAGGA11.639344262295082No Hit
TAGGACCACTCAGTTCAAACAACTGGAGGGATTTAGGACAAGCAGCAATT11.639344262295082No Hit
TCCGTCGCGCCAAACCCGAGCAGCGATCGCGGATCCCGGGTCAACGCAAG11.639344262295082No Hit
CACACGGGTCTTCTTGGTCTCAGGGTTGTGGGAGATAACGGTGGCATAGT11.639344262295082No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA11.639344262295082No Hit
GTCCAACCGCCGATAGCTCGCGGATGCCCCCGGGGGCCCGACGCCGGCCA11.639344262295082No Hit
GTATTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAA11.639344262295082No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCAGACGAGTCCGCCAGTGAGGTGTTGTTAGTTCTGGCATTGGAAGTT11.639344262295082No Hit
GTCCGCTATCTGCAGTGGTGCTGAAACGACCGGAGATACTGACAGATGAG11.639344262295082No Hit
GCCAAAGACTGTTCCAATACCAGCACCAGAACCAGCCACTCCTACTGTTG11.639344262295082No Hit
GTCCTGCGCCAAGATGCTTCAAAGTATTATTAAAAACATATGGATCCCCA11.639344262295082No Hit
GACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAA11.639344262295082No Hit
GGTCTGGGCTTCACCGCCAGCGGCCTTGCCTACTGGGGCAGCGACATCGG11.639344262295082No Hit
CTCTTGCCCTACCGGCGATGATAGCCTTTTGCCGGAAACCAGAGGATCGA11.639344262295082No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)