FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003657954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003657954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.918032786885246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGGCCTGATCTTCGGAAAGCAGGCCACCGTCGGCAAGATCGTGCAGGAA34.918032786885246No Hit
TGGCAGGAGGGCTCACTCAAAGCCTCCTGCCTATACGGGCAGCTCCCCAA34.918032786885246No Hit
GTCCCAGCCCACGGCGCGCACGCCCAAGCGCAGCGCCTTGGGGAACTTGC23.278688524590164No Hit
GCACTGGAGCTCAGCCCAGGTTCCAGCTCAATTGAAGAATCTCCTCCTTC23.278688524590164No Hit
CTCGCGCCCTGACGGCGGGGCAGCCGTTCGAGGCCGCGGCGGACCAGCGG23.278688524590164No Hit
GTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAATGATT23.278688524590164No Hit
CATTTGCTCTTCGTCTTCTGACTCTCCGTCTTCTTGGGCAGCAGCACGGC23.278688524590164No Hit
GGGCACAGATGAGGGCCACACCACGCTTTATCCAGTGTCGCTGGGGCTGA23.278688524590164No Hit
CTCGTAGAGGCCGTGCGCCATGTCGACATGCTGCAGCCCGCCACCGCAGA23.278688524590164No Hit
GTAGAGGCAGTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGA11.639344262295082No Hit
ATCCAGAGTTGCATACAGACCAATTTCTGCATCAGTGTTATCTCGACCAG11.639344262295082No Hit
GTCATCGACCCCGACATCGCCGAACTCGTTGATGATCAGGGCGAGGCGGC11.639344262295082No Hit
ATCCAGTAGTCACACTTCTTTTTCCTAAACAGGACGGAGGTGACATTGTA11.639344262295082No Hit
CGCCGCGTCTGTGAGGAATGGCTGGCGAGGGACCCGGAGAACGCCGGGGC11.639344262295082No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GCTGACCTGGAGTGAAATGTATCTGGACGAAATGTCATCGGCACCTGGCC11.639344262295082No Hit
TCGCCGAGCTTGCCGGCGAATGGGCTGACCGCGGCCACGAGCACCTGTCT11.639344262295082No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.639344262295082Clontech SMART CDS Primer II A (96% over 26bp)
GTATATTATTAGATCTTGTCACACCTTTGCTCAAAACTCCCTAATGGTTT11.639344262295082No Hit
AACTATGGGAGGTGGAGACGGCTCCTTCACATGGCAAAGAGGATGAGAAA11.639344262295082No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA11.639344262295082No Hit
CGCCAGGAACGCAGCACCGCGCCCAGCAGCGCGCCCGCCGCTTCCTCGGC11.639344262295082No Hit
CAACTGAGGTAATTATTTCACAATGATGGGTGGCTCAGTGAGATTCTGAT11.639344262295082No Hit
CGGTGCCACTGCCAGCCAGATCGCGGACGAGCTTGGCGGAGTGAGCCGCA11.639344262295082No Hit
AACCCGTGACGTCGAAGTTGATGCGGATGAATTTGCCGAATCGTGAGGAG11.639344262295082No Hit
ACTCAACCCCGAATTCAACAGGACCGGCATGAAGTCTAACTTCCAATGCC11.639344262295082No Hit
GTCCCAGCGAGTAGAGGTACGGGATCAGCGAATAGCGCAGGCGCAGCCAG11.639344262295082No Hit
ACATTGTTGGCCTTCTTACTAACCTTGGACCGTGATTGAGCCCAAGAAAC11.639344262295082No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAGAAACATCCAATTCTCAAACTGAAGCTCGCACTCTCGCCTCCAGC11.639344262295082No Hit
GTGGGGTGGCAGGTATTAGGGATAATATTCATTTAGCCTTCTGAGCTTTC11.639344262295082No Hit
GAACAGGACCCGTCGCCATGGGCCGTGTGATCCGTGGACAGAGGAAGGGC11.639344262295082No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGCGACAGCAGCGCATCGGCAAGGTCGAGCAGGCTTTCGCCCCTGTC11.639344262295082No Hit
GGACTTTGTTGCCCCTTGGATCGCCCACATCAGAGACGACTTGCCTGATC11.639344262295082No Hit
CCTCGGCGGCGTGGATGCCGAATCGTTCGACGACCGTGGCTTTTACTGTT11.639344262295082No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT11.639344262295082No Hit
TATTTAAAGACTTCTGTAAAGATACCAATTACCATCAAGCTGATCTATAG11.639344262295082No Hit
AAGTGATGTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGAT11.639344262295082No Hit
GTTGAGCCCTTCGTCCTTCACGGGCTCCGCCAGGTTTTCGGTCACCTCGT11.639344262295082No Hit
TCCACATCTACTTCAAGGAATATCACGTTGGAATACTTTTCAGAGAGGGA11.639344262295082No Hit
CATCGGCCGTGAAGCCGTCGTCCGGGTAGGCGACCACGCCGACCGACAGC11.639344262295082No Hit
GGCGGAGGGGCCGGTTTATTTTTAAATTCTGCCTCCACTCGCTCTCTCGC11.639344262295082No Hit
GTGCGCTGGTCGCGGCCGAGGCGGGACTGGTGCTGGGCGGACTGCCGGGG11.639344262295082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAATACAATGC11.639344262295082No Hit
AGCTGACGCTGTCAGGTGTGCAGGCGCCGGCGTCGGGCCGCCTGCGCGCC11.639344262295082No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)