FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
CCGCAATGACGCATCTGGAACTGGTTCCCGTCCCGCCTGTCGCGCAACTG32.727272727272727No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32.727272727272727Clontech Universal Primer Mix Long (96% over 26bp)
CTCTATAAAAGACTTGAAGTTGATTTGGGAGAGGCCAACTGGATAGTAAT32.727272727272727No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA32.727272727272727No Hit
CACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCA21.8181818181818181No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG21.8181818181818181No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT21.8181818181818181No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTGAGGGGCAACGAAAAAAAAAAAAAAAGTACTCTGCGTTGATACTG21.8181818181818181No Hit
GCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCA21.8181818181818181No Hit
GCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.8181818181818181No Hit
GTGTGGCATCGGTCTCGGCCCCAGTGCGCTCTCCCTCAGGAAAGGTCATT21.8181818181818181No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA21.8181818181818181No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA21.8181818181818181No Hit
GGGCATGTCAGATGCAGACAACATCCTCCCTGCAACACCAGGCCCCCTGC21.8181818181818181No Hit
TAGTTGGTGACAGATTTCTATCATGCCAGACCACTGACTGTTCAGTCTTC21.8181818181818181No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC10.9090909090909091No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAACGACGTCGCTATGAACGCTTG10.9090909090909091No Hit
GCGTAACTCTGGAAGCACAGCTTGACTACACGTCATTTTTACCAATGATT10.9090909090909091No Hit
CAGTAAACAGAAACTGGACCCCTTCCTTACACCTTATACAAAAATTAACT10.9090909090909091No Hit
GGTTATGGAGCTGGGGCGGCCTGATGCCGAGTACTGGAACAGCCAGAAGG10.9090909090909091No Hit
GGATAAATAGCTTTCATCTGAACCCTGGTGGTGTGATCTAGAGGTCACTA10.9090909090909091No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC10.9090909090909091No Hit
GGTCAGGAGATCGAGACTTTCCTAACATGGTGAAACCCCATCTCTACTAA10.9090909090909091No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC10.9090909090909091No Hit
GGATTGTGCCTAATGTGTTTGAGCATCGCTTAGGAGAAGTCTTCTATTTA10.9090909090909091No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG10.9090909090909091No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA10.9090909090909091No Hit
GGCCGGGGCCGGAGAGGGACTGACCGGGTTGGTTTTGATCTGATAAATGC10.9090909090909091No Hit
GATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATCACGAAT10.9090909090909091No Hit
GCCCCGCCGCGCGCCGGGATCGGGGTCCGGTGCGGAGTGCCCTTCGTCCT10.9090909090909091No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCTCTTAATCATG10.9090909090909091No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGGAGCAGTGG10.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
TCGCGGAGCGGCGCGCTGAGCAGGGATCCATCTCGCGTGCGGAATGGAAC10.9090909090909091No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC10.9090909090909091No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAATCCACCGCCA10.9090909090909091No Hit
GTGAGACTCTGTCTCTTTAAAAAATTTAAAAATTAGCCAGGTGTGGTGAC10.9090909090909091No Hit
GGTCCGAGGAGCTGCAGGTGTGACTGATGGGAATGAAGTGGCCAAGGCCC10.9090909090909091No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10.9090909090909091No Hit
GATCGCCGGTGCCGCACTGGCTGTCGGCGTGCTGGTGGGCCTGCTGCTTG10.9090909090909091No Hit
CCCCACCTAAAGGTCAAGGCTGGAGAGTGGAATCACCACCTAAAGTTCAA10.9090909090909091No Hit
CGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAA10.9090909090909091No Hit
AATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCTGTCTCTTATA10.9090909090909091No Hit
GGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATG10.9090909090909091Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TACGGCAAGTGCTCGAAATGACATCCAGAGAAATCTAAACTGCTGTGGGT10.9090909090909091No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.9090909090909091Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10.9090909090909091No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC10.9090909090909091No Hit
GCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGC10.9090909090909091No Hit
CGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCA10.9090909090909091No Hit
CCTCTGGCTGCTCTGTCTGGTCGCGTGCTGGCTCCTGGGCGCCGGGGCCG10.9090909090909091No Hit
GGTCAAGCACTCAGCAATGTCCCACAGCGAGCACTGGCCACTGCGGCTGG10.9090909090909091No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.9090909090909091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTGTGCTGACCTTTGAGAGGATTTGAAATTGCTTCATATTGTGATCC10.9090909090909091No Hit
TCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGT10.9090909090909091No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT10.9090909090909091No Hit
ACATAATATGGTAGACTGTCACAGAGCCGAATACCAGTAACAGGATGAAT10.9090909090909091No Hit
TATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCT10.9090909090909091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACC10.9090909090909091No Hit
GTAGTATATGCTCTTTGCAGCATTTTAAGTTATTCAACTTTGTATTTAAG10.9090909090909091No Hit
ATCCGGGAGATCGCGGACGGGTTGTGCCTGGAGGTGGAGGGGAAGATGGT10.9090909090909091No Hit
GAGCATCGGGGCACGCTTCCACGCGATCGGCGGACCCGAAAGGTGGTGGG10.9090909090909091No Hit
GCACGCGCGCTACACTGACTGGCTCAGCGTGTGCAGCCCCGGACATCTAA10.9090909090909091No Hit
CCTCGGCGACGCCGCTTAGCTGGTGGATCACGTCCTCGATCTCGCGGGGA10.9090909090909091No Hit
TTCTCATAGGGTAGCTTTCCTTTTAACACGTGTTTATCCATGACAGTTTC10.9090909090909091No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT10.9090909090909091No Hit
GTGCTATACTGACTAGTAGCGGTGACTGTGAGCATCTTTGTCTTGTTCCC10.9090909090909091No Hit
GGTGATGGCTACTGAAAAGATCCATTGAAAATTATCATTAATGATTTTAA10.9090909090909091No Hit
GAAGTCCCCTCAGCCCCTCAAAGTCCCCCATCACCACCTGATACCCCTCC10.9090909090909091No Hit
GCCGTCCGCCGTCGGCGTCGGCCTCCTTGTCCTTGTCCTGGCCGAGGTCG10.9090909090909091No Hit
TGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCA10.9090909090909091No Hit
GAAGTCCCCTCAGCCCATCAAAGTCCCCCATCACCACCTGATACCCCTCC10.9090909090909091No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCC10.9090909090909091No Hit
CTCATTTAGTTCCCAAGGAGATCAAGGTCAGTTCTCTATGAATGTAAACC10.9090909090909091No Hit
TTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC10.9090909090909091No Hit
GGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGG10.9090909090909091No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.9090909090909091Clontech Universal Primer Mix Long (96% over 25bp)
ACCCCGGCCCGGCTGCCCAGCTCCACCCACGTGACGGGGTGGAGCATCTG10.9090909090909091No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCAAGTAC10.9090909090909091Clontech Universal Primer Mix Long (95% over 24bp)
GTTCCTGCCGAAATGCAAAAATCATGAGGAAACATCAGAGCAACTCAAAC10.9090909090909091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGTAAC10.9090909090909091No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT10.9090909090909091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)