FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCCGAGTTTCAGGCTCGTGCTAAGCTAGCGCCGTCGTCGTCTCCCTT36.976744186046512No Hit
ATAGAATGCAGCTGGAGGCTACGCATGGCCTCTGCAGCACACGCAGCTGG36.976744186046512No Hit
ATAACATCAAGCTCCAGTAGGAAGGTACAGAGAGGGCAGGAAGTTTCCAT24.651162790697675No Hit
CTTCAACACTGCCACATTTCCTTATTGATGCATCTTTTCCCACCCTGTCA24.651162790697675No Hit
GTCCACATCATCTTCATCAGCTACTTTTTCTTTTTCATCTTCATCCTCAG24.651162790697675No Hit
GGCGTACTGGTTATGCCCGCGGAGGAGGCGCCCGTCCGGCCCCACGTCGC24.651162790697675No Hit
AACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGAAGTC24.651162790697675No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA24.651162790697675No Hit
GCTGACGACCGCGTGAACCCCTGCATTGGGGGTGTCATCCTCTTCCATGA24.651162790697675No Hit
GGCTGGAGGTTCAGGGGTTCGGGGCTGGCTCCGGGATCGGCTCAGGATAC12.3255813953488373No Hit
GCCCACAAAAGCCACCAGGAAGAGGAAGACACCCACTGCGATGATGACCA12.3255813953488373No Hit
CGTAAAGACCACGGGGATCGTGGAGACACACTTCACCTTCAAGGACCTAC12.3255813953488373No Hit
CCTGTACAGTAGTGCTACTTAAAATATGCTGGGGACCATCACCATCACAG12.3255813953488373No Hit
TCACGTATTTGTGGTCCCTTAGGAGCACCACCGTAGATACAAGTAGACTT12.3255813953488373No Hit
GCGTGGAGCAGGGCAAGAGCTGCCACAGCGTGGGCCAGCTGGCACTCGAC12.3255813953488373No Hit
AAGTTTGTAATTACTGTGGCCCCTCAGAATGATATTTGGCCTCACGGGAG12.3255813953488373No Hit
GAGTGACACCAGGTCAGGATTTTGCAGGTCCACTGCGAGGTGAAGGGCAG12.3255813953488373No Hit
GAATGCAAGAGGCGTTTCTATAAGAATTGGCATAAATCTAAGAAGAAGGC12.3255813953488373No Hit
CCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCGGAGATCACACTGACC12.3255813953488373No Hit
CCTGTAAACTTCATGGTTCGGCTTTTTGTGGTGATTGTGATGTTTGCCTG12.3255813953488373No Hit
CGCTTGATGTCATCCACCTGCACTTCTGTACCCTTGCGTTTCCGGCACAC12.3255813953488373No Hit
ATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAA12.3255813953488373No Hit
CTCCATTCCTGTCCTTCCACTCCCCTCAAACGTCTTTCATTCAAGGTCAC12.3255813953488373No Hit
GGGCAATGTGGACAATGATGAGCGCCTCCCACCGCCATTTCCTCTGAAGC12.3255813953488373No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGC12.3255813953488373No Hit
GCGTAAGAGTCTTGCCCAGTCTCCCTTCATCCTGGCTTGAAGATAAAGTG12.3255813953488373No Hit
GACTGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCA12.3255813953488373No Hit
GCCCCAGGCTAAGGGTGGCCAGAGAAGGTCACCATGTACCACACACCAAA12.3255813953488373No Hit
GCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTT12.3255813953488373No Hit
AAATGGAACTCTCTGAATTCATTTGACTCCAACGGTTTTCCTTACCAAAA12.3255813953488373No Hit
GCTGCTTAGAGTACTTTGTCCTTTCTCAGTTCTTAAGGGCAACTGGGAAG12.3255813953488373No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)