FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG22.5316455696202533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGGATGCCTTGAGCCTGGGAGATTGAGGCCAGCCTGGGTAACAGTGAC22.5316455696202533No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22.5316455696202533Clontech SMART CDS Primer II A (96% over 26bp)
GGACAAGAAGATGGATGCCCATCCTCCTCGCCTCTTTGCCTGCTCCAACA22.5316455696202533No Hit
AGTATACTCATAAGCTTATTCTTCTTCCTATATTGTTATAGCTCCTTTAT22.5316455696202533No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGTGTATCAACGCAGAGTACAT22.5316455696202533No Hit
GCTTGGTGGTCCATGCCTATATCTTGAAGGGAATCCAACTTAGCTTTAAT22.5316455696202533No Hit
CCAAAAAGGAAATTAAGAAAACAATTTCATTTGCAATATAATAAAAAATA22.5316455696202533No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.5316455696202533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA22.5316455696202533Clontech Universal Primer Mix Long (95% over 21bp)
CGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCG22.5316455696202533No Hit
GCAATAAAGGGCATCCAAACTGGAAAAGAGGAAGTTAAATTGTTCCTCTT22.5316455696202533No Hit
ACAAAATCCTCCACCTAACCTCCCCCCTGGGTCCCTGGTGATGCTCTATG11.2658227848101267No Hit
TTCCTGCGGTTCCCACTTCTCACCGGTGTCACCTTCCCACCGTTCCCACT11.2658227848101267No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.2658227848101267No Hit
GCGGATCTGGTCACCACCACAGGTAGGCGGGCAATGACGGCCGCCGATGT11.2658227848101267No Hit
TCCCTCAGCTATTCAACCCAATGCATGGAAAGCCTCAGTTGAATAAACTT11.2658227848101267No Hit
GTAGGCAAGAGCCAAAGGGACCTGCAGACTCAACTCTCTAGTGGGAGGCA11.2658227848101267No Hit
CTAACAACAGCAGTTGGTTCAAACAGAAGATGCACAGCCCAACAGGGCCA11.2658227848101267No Hit
GTTTAAAAATGCTAGATGATCAATGGCATGCCCTGGCGTTTTAATTACTT11.2658227848101267No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC11.2658227848101267No Hit
GTATCACTGCCAGGTTTCCAGCCAGCTGGGCACACTTCCCCATGTTTGTC11.2658227848101267No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAAATTGATATAATGAAGACTAGTTTTCCAGGTCGTGGTTCAATTCC11.2658227848101267No Hit
GGTTGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAGATATGTGTGATT11.2658227848101267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTGGACAAAG11.2658227848101267No Hit
GGTATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGAAGCAGTG11.2658227848101267No Hit
GGACAAGAAGATGGATGCCCATCCTCCTCGCATCTTTGCCTGCTCCAACA11.2658227848101267No Hit
GCATTCGATTCTATGCGATGATTCCATTCCTTTCCATTAGAAGATGATTC11.2658227848101267No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGAGTA11.2658227848101267Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACAGACTCTT11.2658227848101267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAGAGAAACCAAGTTGGTTTTATGACTTGCAAAGAGCTATTGGACTG11.2658227848101267No Hit
GCTCCTGGTTGGTTCTGGAGCAGATGATCTCAATGAGAGAGTCCTCGTCG11.2658227848101267No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACCTGTCTCT11.2658227848101267Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGCAGTGGGAAGGAAGGCAAGCCTCCTGTCCTCACTGAACTTCCATCCTG11.2658227848101267No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GTGTCGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCG11.2658227848101267No Hit
GTGGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGGGTTTAT11.2658227848101267No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC11.2658227848101267No Hit
CTGTTATTCTCCATCGGAGAACCTTGTTTATTCTCCTCGAAGCACTATTT11.2658227848101267No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT11.2658227848101267No Hit
CCCCCGACGCGTTCGCCCCCGCCCTGGCCTCCGAGGCCGACTCCGTCATC11.2658227848101267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2658227848101267No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAAATGGGAAG11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCATAAAAAAAAAA11.2658227848101267Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTGTTAACAA11.2658227848101267No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
GGGCCTCAGTGAGGACTAACTGGTTCCCTGGGCTGAAAAAAGCTTTTCTT11.2658227848101267No Hit
GTCTTTCTCAAGCACAGTAATTAAGAGCCATGGCTGTGGTATCAGACGCA11.2658227848101267No Hit
GAGCCACTGTGCCCGGCCAGTAGTTTATATTTTTATTCCACCAATTCTTA11.2658227848101267No Hit
TTCCATTCATTGTGCTATTTGTTGCCTGTACCTTTTTTTAAATTGCATTT11.2658227848101267No Hit
CTCCAGACCCTTCACATCAATTTACTGTTGTCTTCGACCTCACCCCAGCA11.2658227848101267No Hit
ATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA11.2658227848101267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGACAGAGTCTT11.2658227848101267No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.2658227848101267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACCTACGAACAATTCCAGCGTCGAGCTCAATACCCCGAAGGTGGGT11.2658227848101267No Hit
GGAGTACAACTGGAACGTGAAGAACAAAGCTAGCAAGGGCTATGAGGAAA11.2658227848101267No Hit
CAGCAACCCAGTGTCAGGGTAATGAGTCACTTCATCTGCCACCTGCAGAT11.2658227848101267No Hit
ATAATGGGCGGCTCAAGGTGCAGCGCCTCTGCTCAGAAATGGAAGAATTA11.2658227848101267No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.2658227848101267Clontech SMART CDS Primer II A (100% over 26bp)
AAATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
CTTACCGAGAGAACCTGCGGATCGCGCTCCGCTACTACAACCAGAGCGAG11.2658227848101267No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCC11.2658227848101267Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATATGCGTTTATA11.2658227848101267No Hit
CTGTGGATAGAAGTAGACATGGGAGACTTTTCATTTTGTTCTGTACTAAG11.2658227848101267No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)