FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC33.79746835443038Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.79746835443038Clontech Universal Primer Mix Long (96% over 26bp)
CCGCTTAGCAGAAGTCAAGGCTTCTGTCTTTTCTTCTTCTTGAGAATCCT33.79746835443038No Hit
GGTGTATAGAAACACTACTGATTTTTGTATGTTGATTTTGTATCCTGTCA22.5316455696202533No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22.5316455696202533Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAA22.5316455696202533Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT22.5316455696202533Clontech Universal Primer Mix Long (96% over 26bp)
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG22.5316455696202533No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.5316455696202533Clontech Universal Primer Mix Long (96% over 26bp)
ACTTATTCCCACGTATTTTATTATTTTTTATTATATTGCAAATGAAATTG22.5316455696202533No Hit
CATGTTTACTTACTGCTCTTGAAGCTGTGCCTATTGACATAGACAAGACA22.5316455696202533No Hit
GATCATGTCTTTTGCAGGAAGATGGATGGAGCTGGAGACAATTATCCTTA22.5316455696202533No Hit
TCACAGGGCCCATATTATCTATATTTCCTGCATTCTTGGCCTCTTTTTAG22.5316455696202533No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.2658227848101267Clontech SMART CDS Primer II A (96% over 26bp)
GTCTTTGAGTATGTGTTGGTGGAGGTGACTGGAGGACTCTGATGGCAGAG11.2658227848101267No Hit
ATTGGGCACCCTGCCCCCAACTTCAAAGCCACAGCTGTTATGCCAGATGG11.2658227848101267No Hit
CGCTTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT11.2658227848101267No Hit
CCCTTAACACAGGACTGAAATATGGCAGTTTCTTAAGTCACTGGACTTGC11.2658227848101267No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACTTTTTTTTTT11.2658227848101267Clontech Universal Primer Mix Long (95% over 24bp)
AAGTGATCTTTTCCTTGCAGACATCCATTCTCAGGGAATGCTGTTACCAG11.2658227848101267No Hit
GTTCGGCGGTCCGATATCCCGCCAACGGGTGCAGCACCACGACGGCAGGA11.2658227848101267No Hit
GTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCTGTATCT11.2658227848101267Clontech Universal Primer Mix Long (95% over 21bp)
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC11.2658227848101267No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267Clontech Universal Primer Mix Long (95% over 22bp)
GCCCTGCCTGTGTTCCCGACAAGTCTTCCTTATTTTCAAACTCCATGCGG11.2658227848101267No Hit
CTCCTGCACAGGACTCAGGTTCTAGTGGTAGAAACAGATACGCAAGTTCA11.2658227848101267No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
CTTCAGCCTCCTCAAATGAAACAATTATCAGCAAAGAGTTTTATTTCCAG11.2658227848101267No Hit
GAAAGAGAGGCTTTTGCTTTCAAAGTGCTCATAAATTCCCAGGGAAAGAG11.2658227848101267No Hit
GCTTCCCATGTACTCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.2658227848101267No Hit
AGATAGGCCTTACCAAGAAAAGTGTTTTGAAAACAATTGGGCCTTCTAGT11.2658227848101267No Hit
GAATGGAATCCTCATTGAATGGAAATGAAAGGGGTCATCAACTAATGGAA11.2658227848101267No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
GGCCACGTCCCTGTCACCTTCCTCCCGTTCACTCTCGCTGCCCGAGTGCT11.2658227848101267No Hit
CCCAGTTAGCAATGTAGGGGCCAATGGCGGCAAGCAAGACGACCAGCGCC11.2658227848101267No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTGA11.2658227848101267No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTT11.2658227848101267No Hit
GCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAA11.2658227848101267No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGGACGTTTTTAAAC11.2658227848101267No Hit
AGGTAACGCCGTGAGGAGAAGTGGGAATGGCAGGAAGGTAACGCCAGTGA11.2658227848101267No Hit
GGATTACAGGCATGAGCCATTACACTGGACCCTCTTTACCATTTCAAAAT11.2658227848101267No Hit
ACGCCGCGCACGCCGTCGAGACCCGTGAGCGCCTCGAGGTCCCGCTGCCA11.2658227848101267No Hit
CTATTGAAGACACCTGCTCAGTATGACGCTTCTGAGCTAAAAGCTTCCAT11.2658227848101267No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.2658227848101267Clontech Universal Primer Mix Long (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267Clontech Universal Primer Mix Long (95% over 21bp)
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT11.2658227848101267No Hit
GCATTTATTCGCTACATAGATCAGTATCTCCTCCAAGTTCAAGTCTTCAA11.2658227848101267No Hit
AATCCATTTAACCCTGAGTGGACACAGCACATGTTTCAGAGAGCACAGGG11.2658227848101267No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.2658227848101267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGATGCAAGCCAGGTTCTCAATTTGCTGGATCTTATTCCCTTGCAGATA11.2658227848101267No Hit
CCACACGGGCCGCCTCCCACTTGCGCTGGGTGATCTGAGCCGCCGTGTCC11.2658227848101267No Hit
CAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCGG11.2658227848101267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGCGTTTAAACCA11.2658227848101267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC11.2658227848101267No Hit
GTACTAATCGATACAGGAGCCAAATCACTAGATAGATTTTTTAAACCGTT11.2658227848101267No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.2658227848101267Clontech Universal Primer Mix Long (96% over 25bp)
GGCTTCGACATCTTGGCTTACAGGGGCAGGGTCCCGAATAACGATATCTG11.2658227848101267No Hit
ATCTATTAGGAAAGATGAAAGGAAGGAATTCCCCAGTGTTCACGCTGAGA11.2658227848101267No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267Clontech Universal Primer Mix Long (95% over 21bp)
GGGGAGTGGGGCTTTTCTTCTTTCTCTATCATTTTTCTTCCTTCACCACA11.2658227848101267No Hit
GAAGGCAGAAGCACCTGCAAGAAGGGCAAAGAGGAGGGAGGCAGATGGGA11.2658227848101267No Hit
GTCTAGATCCATCGGGTTTCCTAAGAGCAACTGCAACAAAATGGTGCTCA11.2658227848101267No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)