FastQCFastQC Report
Tue 10 May 2022
EGAF00003658071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372423
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63670.463924023424265No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT62020.45190149101261057No Hit
GCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC45300.33007316257451236No Hit
CTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCT41720.30398791043286216No Hit
GTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGGCCTGGATCCG37370.2722921431657732No Hit
CTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCC34310.24999581032961413No Hit
CATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG33890.24693552935210208No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT33670.24533252503054817No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCT33490.24402097604018588No Hit
GTCATACAGTTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGG31050.22624220083749688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29760.21684276640656705No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC27830.20278004667657132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27630.20132277002061316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27570.20088558702382572No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27370.19942831036786762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26270.19141328876009803No Hit
CCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACCAG24960.18186812666357238No Hit
GTCTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGG23390.1704285049143012No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22710.16547376428404362No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22610.16474512595606455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21310.15527282769233683No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21120.15388841486917662No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21050.1533783680395913No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTTGAGTG20660.15053667856047295No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGT20410.14871508274052533No Hit
GTATTAACAAGAATTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCT19880.14485329960223634No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19500.14208447395591592No Hit
CATCTGCACTGTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGG19120.13931564830959553No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19050.13880560148001017No Hit
GTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCTGG19020.13858700998161647No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18950.1380769631520311No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18480.13465236301052955No Hit
CTCCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACC18470.13457949917773165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18460.13450663534493376No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18070.13166494586581542No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT17650.12860466488830338No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC17340.1263458860715683No Hit
ACCTTCTACAGTCCGTCCCTCAGGGGTCGCGTCACCATATCCGTGGACAC17290.12598156690757878No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGCTCAGTCAGA17090.12452429025162066No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16530.12044391561493795No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16310.11884091129338403No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGT16240.1183308644637987No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16160.11774795380141545No Hit
ATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGG16100.11731077080462803No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15810.11519771965348875No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTG15800.11512485582069086No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG15320.11162739184639138No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15300.11148166418079558No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15220.11089875351841233No Hit
CTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTT15190.11068016202001861No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15130.11024297902323117No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15080.10987865985924165No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14890.10849424703608145No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14730.10732842571131494No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14620.106526923550538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14440.10521537456017568No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14430.10514251072737779No Hit
CTCATAAGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCT14390.10485105539618615No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTCTA14200.10346664257302594No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14130.10295659574344061No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14120.10288373191064272No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT13990.10193650208426995No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13910.1013535914218867No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13900.10128072758908879No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13830.10077068075950343No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13830.10077068075950343No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA13820.10069781692670554No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13800.10055208926110973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)