FastQCFastQC Report
Tue 10 May 2022
EGAF00003658072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372423
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT64630.47091895137286394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64090.466984304401777No Hit
GCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC47750.3479248016099992No Hit
CTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCT40990.2986688506386151No Hit
CTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCC36350.2648600322203869No Hit
GTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGGCCTGGATCCG35440.2582294234357775No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT34270.24970435499842247No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCT32380.2359330905996183No Hit
CATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG32370.23586022676682045No Hit
GTCATACAGTTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGG29960.21830004306252515No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29510.21502117058661946No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29390.21414680459304458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28670.20890060863159535No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC28440.20722474047724354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28370.20671469364765818No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25930.18893591844496924No Hit
CCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACCAG25140.1831796756539347No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23880.17399883272139857No Hit
GTCTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGG23820.17356164972461113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23520.17137573474067397No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23350.17013704958310957No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21060.15345123187238918No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTTGAGTG20870.152066819049229No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA20810.15162963605244156No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20640.15039095089487717No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGT20470.14915226573731277No Hit
CTCCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACC20060.14616484859259865No Hit
GTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCTGG20040.1460191209270028No Hit
GTATTAACAAGAATTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCT19790.1441975251070552No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG19250.14026287813596827No Hit
CATCTGCACTGTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGG19190.13982569513918086No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19180.13975283130638294No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18990.13836841848322276No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18300.13334081402016723No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18010.131227762869028No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17850.1300619415442615No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17830.12991621387866567No Hit
ACCTTCTACAGTCCGTCCCTCAGGGGTCGCGTCACCATATCCGTGGACAC17620.12838607338990965No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17620.12838607338990965No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17090.12452429025162066No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGCTCAGTCAGA16960.1235770604252479No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16800.1224112391004814No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16400.11949668578856518No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16280.11862231979499031No Hit
ATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGG16180.11789368146701126No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGT16060.11701931547343639No Hit
CTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTT15780.11497912815509503No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG15640.11395903449592437No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTG15570.11344898766633901No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15430.11242889400716835No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15310.11155452801359347No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15310.11155452801359347No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15270.11126307268240186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15100.11002438752483745No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15040.10958720452805001No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15010.1093686130296563No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14960.10900429386566679No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14790.10776560870810238No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14740.10740128954411286No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14720.10725556187851705No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTCTA14670.1068912427145275No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14610.1064540597177401No Hit
CTCATAAGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCT14580.10623546821934637No Hit
CTCCCAGGCTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCA14320.10434100856660082No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT14290.1041224170682071No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC14090.102665140412249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14070.10251941274665317No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13850.10091640842509926No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13800.10055208926110973No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13790.10047922542831182No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG13750.10018777009712021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)