FastQCFastQC Report
Tue 10 May 2022
EGAF00003658124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.7297401101011394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.25604916143899625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA160.20483932915119704Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCTGGACAGTGGCCCTGCAAGCAGCCAAGGATGGGGTCTCCACCACCT120.15362949686339777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.14082703879144795No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100.12802458071949813Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.11522212264754833Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA90.11522212264754833No Hit
GGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCC90.11522212264754833No Hit
GTACACATTCGGGTGAAATAATGGTGGTTCGAATTTACATTTTGGTGGCG90.11522212264754833No Hit
GTACATGGGACGGCCCGTTTTCCGCCAGCTCGCCGCTCGCTATGGCGTCG80.10241966457559852No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)