FastQCFastQC Report
Tue 10 May 2022
EGAF00003658145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339391
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9520.2805024293513971No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG9250.27254700330886794No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG8840.26046654154058296No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8400.24750214354535036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7910.23306451850520493No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7500.22098405673691995Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6510.1918141612476465No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6120.18032299029732668No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT5800.17089433720988478No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA5590.16470678362125102No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5490.16176032953142538No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG5250.154688839715844No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC5230.15409954889787886No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5020.1479119953092451No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT4800.14142979631162877No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT4590.135242242722995No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT4470.13170649781520427No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4440.13082256158825661No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA4400.12964397995232638No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4380.12905468913436124No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4360.1284653983163961No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG4320.1272868166804659No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT4300.12669752586250077No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG4250.12522429881758795No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA4150.12227784472776237No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4020.11844745441098908No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA3930.11579564573014606No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT3840.11314383704930302No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT3790.1116706100043902No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA3750.11049202836845998No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG3610.10636699264270413No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC3570.10518841100677388No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG3560.10489376559779134No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC3510.10342053855287854No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC3500.10312589314389597No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC3470.10224195691694829No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC3410.10047408446305295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)