FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA412.903225806451612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGTTAGAGTTGTGCTAAGAGATGGTGGAAACACAGGGGAGGGCATGAGA39.67741935483871No Hit
CCATAATCCAGGGGGCTACCCCTGGCTCTCTGTTGCCAGTGGTCATCATC26.451612903225806No Hit
GAGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACT26.451612903225806No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26.451612903225806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAACAGAGACGACACAGATTACCAGTATCTGGAATGCAACAGAGATCTCA13.225806451612903No Hit
CCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACT13.225806451612903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT13.225806451612903No Hit
TCCATAGGACATTTGGCAGTATCTGGAAACATTCTTGATTTTCACGCTTG13.225806451612903No Hit
TCTACACACTTGTAAACCCCAAAATCAGTATTATTTTTGCCTAAAACAGT13.225806451612903No Hit
CTGTTGGCATTTGGCAGGTGGATAGCTCTCCTATTTGCAGGAGGGAGTGA13.225806451612903No Hit
CTTCTCTTCACGGACCTAATCTCCTTGACCAAGCTGAATGCTAGTGTTTT13.225806451612903No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA13.225806451612903No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13.225806451612903Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13.225806451612903Clontech SMART CDS Primer II A (96% over 26bp)
CCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCA13.225806451612903No Hit
CCATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGT13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT13.225806451612903Clontech SMART CDS Primer II A (100% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGCTC13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)