FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT39.67741935483871Clontech Universal Primer Mix Long (96% over 26bp)
CACACACACACATACATGTACACGTAAAATTGCATCCATGGTTACCAATC39.67741935483871No Hit
GTTTTTCGGGTAATTCTCCATCTGCTGCCGGAAGTTGTTATTAAACTCTG26.451612903225806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26.451612903225806Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGGA13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAACTGCGTTTAT13.225806451612903No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG13.225806451612903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGCTGATT13.225806451612903No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC13.225806451612903No Hit
GCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGT13.225806451612903No Hit
AAGTTGACTAGATCTTCTAAAGCTAAAGAGATAAGAAGCAAGCAAAGTTA13.225806451612903No Hit
GAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAAT13.225806451612903No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAA13.225806451612903No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13.225806451612903No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGGTGCTA13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13.225806451612903No Hit
CTGACACACTGTGATGGAAAAGGATGACTTGGCTCAGAGTTTGTGAAATG13.225806451612903No Hit
GCAGATACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTAGCTTTTT13.225806451612903No Hit
GGTGTTCATAGTACTCTATTTTGCTGTCTGCAGAGTCTGCCATGAGATCT13.225806451612903No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC13.225806451612903No Hit
CTAAATAAAAATGACTGAATGATTGCAGACTTTGCATCAGAAACCATGGA13.225806451612903No Hit
GCACCTGTCAGGTTGGCCCCCTTCCTATGGCCTTTTGCCAAGGGTCTGTA13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)