FastQCFastQC Report
Tue 10 May 2022
EGAF00003658355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA971.6975848792439623No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT560.9800490024501224No Hit
GATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGT260.4550227511375569No Hit
TTCCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGAC170.2975148757437872No Hit
ATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAA170.2975148757437872No Hit
ATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTT170.2975148757437872No Hit
GTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGAC170.2975148757437872No Hit
GTGATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATT160.280014000700035No Hit
GTCTTGCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCAC150.2625131256562828No Hit
GATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTG150.2625131256562828No Hit
GTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACT140.2450122506125306No Hit
GTTCTAAAGAGTGGTCAGCTGGTAAGTATGCCATGATCATTCAGATGCCT130.22751137556877846No Hit
GCAGAAGGTCGCGCACCGCGCCGCCCACCACGTAGGCCTCGTAGCCTGCC130.22751137556877846No Hit
CCCTTAACGGGTATGAGGTTAGAGGACTAGAAGGTGACAGAGGTGGACAT110.19250962548127407No Hit
GGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCC110.19250962548127407No Hit
GGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACCTCCTG110.19250962548127407No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG110.19250962548127407No Hit
CCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTGGAGCTTT110.19250962548127407No Hit
ACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGA110.19250962548127407No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC100.17500875043752187No Hit
GCTGGTAAGTATGCCATGATCATTCAGATGCCTCATTTCCTCCTGGGCGT100.17500875043752187No Hit
TCCTAGAGGAGTCAAATTCATAGAAAGGAGAATCGTGGTGGGCATGGCTG90.1575078753937697No Hit
ATCTCATGCTGTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCC90.1575078753937697No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT90.1575078753937697No Hit
TTCTTACATACACCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCC90.1575078753937697No Hit
GGCCAGGTTGGTCTCGAACTCCTGACCTCAAGTGATCCACCTGCCTCAGC90.1575078753937697No Hit
CTACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGT90.1575078753937697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1575078753937697No Hit
GTCTCGTTCTGTCTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCAC80.1400070003500175No Hit
GTACATGGGGTGGTGCGCTCGCCGCTGAAGGTCGACGTGGTGCGCGACCG80.1400070003500175No Hit
AGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGA80.1400070003500175No Hit
GACTATAGGCGTGAGCCACCATGCCTGACCATAAATGCTCTTTTATCATT80.1400070003500175No Hit
CTACAGGGACGTGCCACCACACTGACTAATTTTTTATTTTTAGTAGAGAT80.1400070003500175No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG80.1400070003500175No Hit
CCTTCACACTTTACGCCCAGGAGGAAATGAGGCATCTGAATGATCATGGC80.1400070003500175No Hit
GGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACTCAGCA80.1400070003500175No Hit
CTCCAACTACAGCTGCAGTTCCTGTTATGACCTGCTTCTATCCCAATCAG80.1400070003500175No Hit
GTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCCAAGAT80.1400070003500175No Hit
CCCTCATGGTGGTGGTGATTTCTTTCCATTCCTGTAGTCTGCTGGTAAAG80.1400070003500175No Hit
CATGATAGGTGCCCACTCAATGCTGGTTCTGCCACTCAGCACAAGCAAAT80.1400070003500175No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT80.1400070003500175No Hit
CTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCACTGCAACCTCCGC80.1400070003500175No Hit
CCTCTAAGGTGTATGTAAGAAAGTTTGGAAGTTTTAGGTCAAAATGTGGT80.1400070003500175No Hit
GTAGAGAGGGGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAAC80.1400070003500175No Hit
CCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTG80.1400070003500175No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC80.1400070003500175No Hit
TTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATA70.1225061253062653No Hit
ATTTCATACAGCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTT70.1225061253062653No Hit
ATGCTAGGCTTGGGCCCTGGCACAGCAGTTGGCTCTGGAAGAGGCCCTCA70.1225061253062653No Hit
CCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAACCTCAGGTGATCCAC70.1225061253062653No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT70.1225061253062653No Hit
TCTCTCTTGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCG70.1225061253062653No Hit
GAATGAGTAGGGGCAGGAGCTTCAAGAGGAACTGGGATGGAAGAAGTTGG70.1225061253062653No Hit
TCTTAAGACAGGTTCTATGAGCACATGGTTTTGAGGGCTCAGCAAATCCC70.1225061253062653No Hit
GCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATATAGACAAATC70.1225061253062653No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG70.1225061253062653No Hit
GGTTAGAGGACTAGAAGGTGACAGAGGTGGACATCAGAGGATGGTAATTG70.1225061253062653No Hit
CATCTAATCAGTCAACGTATACTATCGACTCTAATTCCATTATCAGTCAA70.1225061253062653No Hit
CTTCTATCCCAATCAGTTTCTCCTGCCCCGTTTCGAGCAAGGCACCACAA70.1225061253062653No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT70.1225061253062653No Hit
ACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGA70.1225061253062653No Hit
GTCTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCACTGCAACCTCC70.1225061253062653No Hit
GATATGGTGAGCTTCCCAAAGACAGTGAGGGGCACCAGCAATAAATAAAA70.1225061253062653No Hit
TACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAG60.10500525026251313No Hit
CCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAG60.10500525026251313No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA60.10500525026251313No Hit
ATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAA60.10500525026251313No Hit
GTTATGACCTGCTTCTATCCCAATCAGTTTCTCCTGCCCCGTTTCGAGCA60.10500525026251313No Hit
GTCTGGAATGAATGCAGCAACCGGTTCGACGATGAACGCGCTGGCCGCCG60.10500525026251313No Hit
CCTCTACACCGCCGTGCAGCAGCACGCGGCCTTCAACTGGTTCGTCAACG60.10500525026251313No Hit
ACAGAGAACAATCCAAAAGATTTGCTCCTTGGTCCATGGTATGAAAAATG60.10500525026251313No Hit
CTATAGGCGTGAGCCACCATGCCTGACCATAAATGCTCTTTTATCATTCC60.10500525026251313No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT60.10500525026251313No Hit
AGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCCGGATTGTGGTGCCTTG60.10500525026251313No Hit
GTTTGGATCCTTTACCAGCAGACTACAGGAATGGAAAGAAATCACCACCA60.10500525026251313No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA60.10500525026251313No Hit
ATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGA60.10500525026251313No Hit
AGCTTAGGGCTTACATCCTAGGTTCTTTGACCTTAGGGGGTTCCCTCAAG60.10500525026251313No Hit
CAATCAGGGAACCAGATTGTTTTGAAAGGGTTGTGACAGGCGTAGTTCAA60.10500525026251313No Hit
GTCAAAAGTGTCCCCTCCTGTTTGGATCCTTTACCAGCAGACTACAGGAA60.10500525026251313No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT60.10500525026251313No Hit
GTGAGATGCCGACTGCTAATGCCCACCTTTAGACACAGGTCCAGGGCCAG60.10500525026251313No Hit
AAATAAGCAGTGAGACCACTATTTTTTCTACTTCCAAAACAAACCCTGAG60.10500525026251313No Hit
TATCAACGCAGAGTACATGGGGAGTGTTTCTGGAGGCGTTTGGCACACCC60.10500525026251313No Hit
GTGAAATAATTTGCCAATTTCATACAGCTACTAAGCGGTAGAGTTGGGAT60.10500525026251313No Hit
GTACATGGGAATGCCAGGGACCTTACCCGTCCTCCCCAAATGAGTATAAC60.10500525026251313No Hit
CTCCTGACCTCAAGTGATCCACCTGCCTCAGCCTCCCAAAGTGCTGAGAC60.10500525026251313No Hit
GTATGAAAAATGGTCTACCTGTGACTATCCAAGGCTGGGCACGGTGGCTC60.10500525026251313No Hit
GACTACAGGAATGGAAAGAAATCACCACCACCATGAGGGCGTTAGGAATG60.10500525026251313No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG60.10500525026251313No Hit
ATCTTGGCTCACCGCAGCCTCCACCTCCTGGGTTCAAGTGATTCTCCTGC60.10500525026251313No Hit
GCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTT60.10500525026251313No Hit
ACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCC60.10500525026251313No Hit
ACTCTCAGGTTTCTGGGTAGGAAGTCCGGATTGTGGTGCCTTGCTCGAAA60.10500525026251313No Hit
CATCCTAGGTTCTTTGACCTTAGGGGGTTCCCTCAAGTCAGTACCCAGTG60.10500525026251313No Hit
GTGCTGGGGTTACAGGCGTGAGCCACCGCGCCCGGCAAGGCCAAGTGCTC60.10500525026251313No Hit
GTCAGAGACAGTGGGGATTAAAACAGTCCCTTAACGGGTATGAGGTTAGA60.10500525026251313No Hit
AAATAATACTTAACCACAGCACCGCAAACCGGCTCCTGGAATCACTAGAA60.10500525026251313No Hit
ATGCTGAACTGCATGAGGGCCTCTTCCAGAGCCAACTGCTGTGCCAGGGC60.10500525026251313No Hit
GAGTACATGGGGTGGTGCGCTCGCCGCTGAAGGTCGACGTGGTGCGCGAC60.10500525026251313No Hit
TGCCACCACACTGACTAATTTTTTATTTTTAGTAGAGATGGGGTTTCACC60.10500525026251313No Hit
GTACATGGGGGCGGAAGGGGCGCCGGCAACCATGTTGGCCGGGCCGATAG60.10500525026251313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)