FastQCFastQC Report
Tue 10 May 2022
EGAF00003658356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA681.1900595029751488No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT430.7525376268813441No Hit
GGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCC260.4550227511375569No Hit
ATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAA210.36751837591879594No Hit
TTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAA200.35001750087504374No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT180.3150157507875394No Hit
GATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGT170.2975148757437872No Hit
GTGATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATT160.280014000700035No Hit
AGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGA150.2625131256562828No Hit
GGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACCTCCTG150.2625131256562828No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG150.2625131256562828No Hit
ACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGA150.2625131256562828No Hit
CCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTGGAGCTTT130.22751137556877846No Hit
TTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATA120.21001050052502626No Hit
TTCCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGAC120.21001050052502626No Hit
GTCTTGCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCAC120.21001050052502626No Hit
GCAGAAGGTCGCGCACCGCGCCGCCCACCACGTAGGCCTCGTAGCCTGCC120.21001050052502626No Hit
TCCTAGAGGAGTCAAATTCATAGAAAGGAGAATCGTGGTGGGCATGGCTG110.19250962548127407No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG110.19250962548127407No Hit
TTCCTAACGCCCTCATGGTGGTGGTGATTTCTTTCCATTCCTGTAGTCTG110.19250962548127407No Hit
GTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCA110.19250962548127407No Hit
GTCTAAAGGTGGGCATTAGCAGTCGGCATCTCACTGCTATCACTGTGGAT100.17500875043752187No Hit
GTCAAATATTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATC100.17500875043752187No Hit
GTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACT100.17500875043752187No Hit
GTCTATATGAATTGATTCCCGGGTAGCGCATATGAATTATTAAGCAGTCG100.17500875043752187No Hit
GCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATATAGACAAATC100.17500875043752187No Hit
GATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTG90.1575078753937697No Hit
ATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTT90.1575078753937697No Hit
GACTACAGGAATGGAAAGAAATCACCACCACCATGAGGGCGTTAGGAATG90.1575078753937697No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT90.1575078753937697No Hit
GCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTT90.1575078753937697No Hit
GGCTAAGATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGC90.1575078753937697No Hit
GGTTCTATGAGCACATGGTTTTGAGGGCTCAGCAAATCCCTTATCCATTG80.1400070003500175No Hit
GTCTCGTTCTGTCTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCAC80.1400070003500175No Hit
ATTTCATACAGCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTT80.1400070003500175No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA80.1400070003500175No Hit
CTTGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCC80.1400070003500175No Hit
CTTTTATCATTCCTAACGCCCTCATGGTGGTGGTGATTTCTTTCCATTCC80.1400070003500175No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT80.1400070003500175No Hit
GTCAAAAGTGTCCCCTCCTGTTTGGATCCTTTACCAGCAGACTACAGGAA80.1400070003500175No Hit
GGATAAAGGTGAAAGCATTTTCATAGTTGAAGCAAATCTTAAGACAGGTT80.1400070003500175No Hit
ATCTTGGCTCACCGCAGCCTCCACCTCCTGGGTTCAAGTGATTCTCCTGC80.1400070003500175No Hit
CCATGAGGGCGTTAGGAATGATAAAAGAGCATTTATGGTCAGGCATGGTG80.1400070003500175No Hit
CTTTTGACTGATAATGGAATTAGAGTCGATAGTATACGTTGACTGATTAG80.1400070003500175No Hit
TCCAAGATGTGAAATAATTTGCCAATTTCATACAGCTACTAAGCGGTAGA70.1225061253062653No Hit
ACAGAGAACAATCCAAAAGATTTGCTCCTTGGTCCATGGTATGAAAAATG70.1225061253062653No Hit
GTACATGGGGTGGTGCGCTCGCCGCTGAAGGTCGACGTGGTGCGCGACCG70.1225061253062653No Hit
TCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGA70.1225061253062653No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC70.1225061253062653No Hit
ACATACACCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTG70.1225061253062653No Hit
CTCCAACTACAGCTGCAGTTCCTGTTATGACCTGCTTCTATCCCAATCAG70.1225061253062653No Hit
GATTAGCCTGGGAAAGGAAAAAAAATTAAGAGCACTTGGCCTTGCCGGGC70.1225061253062653No Hit
GTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCCAAGAT70.1225061253062653No Hit
ACGTATACTATCGACTCTAATTCCATTATCAGTCAAAAGTGTCCCCTCCT70.1225061253062653No Hit
GAAACAAAGCATCCAAGATGTGAAATAATTTGCCAATTTCATACAGCTAC70.1225061253062653No Hit
CATCTAATCAGTCAACGTATACTATCGACTCTAATTCCATTATCAGTCAA70.1225061253062653No Hit
ACCTTACCCTCCTGTAATTTGGTTGGTAAATAATACTTAACCACAGCACC70.1225061253062653No Hit
ACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCCAAGATGT70.1225061253062653No Hit
GAAATATCTCATGCTGTCTATCTAACTCACAACAATTCCATAGGTAAGTA70.1225061253062653No Hit
CCTCATGATCCTCCCACTTTGGCCTCCCAAAGTGCTGGGGTTACAGGCGT60.10500525026251313No Hit
CATCTAAATAAGCAGTGAGACCACTATTTTTTCTACTTCCAAAACAAACC60.10500525026251313No Hit
GATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATTCC60.10500525026251313No Hit
CAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGG60.10500525026251313No Hit
CTACAGGGACGTGCCACCACACTGACTAATTTTTTATTTTTAGTAGAGAT60.10500525026251313No Hit
CCCTACGGCAGCGTGCCCGCCGGATCGCACACGGTGCACTTCGGCATCCG60.10500525026251313No Hit
GTCGTATAATATTTGACTTTCTCTCTTGTTTTACAGGCAATCTGTGAGAA60.10500525026251313No Hit
GGCTGTAGTGCAGTGGCGTGACCTCAGCTCACTGCAACCTCCGCCTCCTG60.10500525026251313No Hit
ATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGA60.10500525026251313No Hit
TATCAACGCAGAGTACATGGGAGCAGGTCGATCGGCAGCGGGGCCGGCAC60.10500525026251313No Hit
TGCTAAAAGGCAGGCACAGTGATAGGCATTTGAAATATCTCATGCTGTCT60.10500525026251313No Hit
GATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTG60.10500525026251313No Hit
AAACAGGAGGGGACACTTTTGACTGATAATGGAATTAGAGTCGATAGTAT60.10500525026251313No Hit
GTTCCAAAACGTTTCCTCTCTCCAACTACAGCTGCAGTTCCTGTTATGAC60.10500525026251313No Hit
CAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAA60.10500525026251313No Hit
GATCCAAACAGGAGGGGACACTTTTGACTGATAATGGAATTAGAGTCGAT60.10500525026251313No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG60.10500525026251313No Hit
CGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGAC60.10500525026251313No Hit
TTTTAGTAGAGAGGGGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTT60.10500525026251313No Hit
TACATAAGCTCACCTCACAGATTAGCCTGGGAAAGGAAAAAAAATTAAGA60.10500525026251313No Hit
CTACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGT60.10500525026251313No Hit
CCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTG60.10500525026251313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)