FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATCGAGCAGGCCTGCGGCCGTGTCGCTGATCTGCAGGGTGCGCCAGG35.660377358490567No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA35.660377358490567No Hit
TTACTATTGGTGGCACCGCACAGATTCAGTTTCTGTCTCTTATACACATC23.7735849056603774No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA23.7735849056603774No Hit
ACCCATGCGGAGCCGCCGCGGCCTTCTGGCTCGTGGAGCACGGCGATCTC23.7735849056603774No Hit
GTATCAACGCAGAGTACATGGGGAGATCTACACTTTTTTTTTCGTCGGCA23.7735849056603774No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGT23.7735849056603774Illumina Single End PCR Primer 1 (95% over 24bp)
GTTCTCCGCCACCACGACGCTGTCGCGCAGCAGGCGCGCCTTCGCGTTGC11.8867924528301887No Hit
ATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAGAGTACATGGG11.8867924528301887Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATGGCGATGCGTTCGCCACCTACCACCGTGGCTTTCTCTCTGCTACTGAA11.8867924528301887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCCACCCTGCCGTCGCCACCCAT11.8867924528301887No Hit
GCCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT11.8867924528301887No Hit
AGCGGGAGGAGTGGGTGGCCCCGATGCCGGGCCGGCCATGCTTTACGGGC11.8867924528301887No Hit
TCGGAAGAGGTTCGTGTAGGGAAAGAGTGTAGACTCGGTGCGCCGTTCAC11.8867924528301887Illumina Single End PCR Primer 1 (95% over 22bp)
AGATCTCGGTGGTCGCCGTATCCCATGTACTCTGCGTTGATCTGTCTCTT11.8867924528301887Illumina Single End PCR Primer 1 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCTGTCTCTTATACAC11.8867924528301887No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA11.8867924528301887No Hit
AGTCTACACTAAGCCTTCGTCGGCAGCGTCAGAAGTGTATAAGAGCTGTC11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATG11.8867924528301887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAATACCATCGCAAAAAAAAAAAAAA11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCAAG11.8867924528301887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTA11.8867924528301887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.8867924528301887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAT11.8867924528301887No Hit
GTGTAGATCTCGGTGGTCGCCCCATGTACTCTGCGTTGATACTGTCTCTT11.8867924528301887Illumina Single End PCR Primer 1 (95% over 21bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAACTGTCTCTTA11.8867924528301887No Hit
GGTTCCAGGCCGAGACAATCAGACGGCGCGAATCGGGGTTGCGCTTGATC11.8867924528301887No Hit
CCACTAGAGCGTGTGGCACGCCGGGGGTCCCGCAGCATCCTGTGAGCATT11.8867924528301887No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAA11.8867924528301887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAATCCCCTCTCAAAAAAAAAAAAAA11.8867924528301887No Hit
GTACATGGGAAGCTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
TCGCTGACGCGTCCGCCCGCGCAGGTCGAAGCGCCGGTGCCCGCGCAATG11.8867924528301887No Hit
CTGTCGATGCCGGATGCCTTCTGCAAGACGTGCAGGAGGCAGCACGCGCT11.8867924528301887No Hit
GCTTCGACTGATGAAGGTGGTAAGCATCCCCCGTCGGGGGGCGACCGCCG11.8867924528301887No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGATCACCCGAGCTGTCTC11.8867924528301887No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GTCGGGGACGGCTGGAACGATGCGGAGGTCAAGCTGGCCGGCGCCGCCGA11.8867924528301887No Hit
GTGTAGATCTCGGTGCGCGACCCATGTACTCTGCGTTGATACCCTGTCTC11.8867924528301887No Hit
GCGGTCTCGGCCGCGTCCCGGTGGGCGCGCAGCTGCGTGAAGAGCGTTGC11.8867924528301887No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA11.8867924528301887No Hit
GGCGGGGCACAACGTCGTGCTGGCCGATGGGCGACACCACACCTTCTCGC11.8867924528301887No Hit
GCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GCTCTTCCGATGATGTAGATCTCGGTGGTCGCCCATGTACTCTGCGTTGA11.8867924528301887Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)