FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGGCGCACGTCCATCGACGGCGTCGGCTCGGCCCAGACGCCGATGA35.660377358490567No Hit
AAACTGAATCTGTGCGGTGCCACCAATAGTAACTGTCTCTTATACACATC23.7735849056603774No Hit
CTGCCGACGAAAAAAAAAGTGTAGATCTCCCCATGTACTCTGCGTTGATA23.7735849056603774No Hit
GGAATGCGCCGAGGGCTCGCCGATGCGCACGGGCACGCGCACGTCGCGCA23.7735849056603774No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGT23.7735849056603774Illumina Single End PCR Primer 1 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAG11.8867924528301887No Hit
GTGGAACGGCGTGGTCACGCGGCAGCGCGCGTCGATCGACAGTCGTGCAA11.8867924528301887No Hit
GATCTACACTCTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAA11.8867924528301887No Hit
ATCAACGCAGAGTACATGGGATACGGCGACCACCGAGATCTCTGTCTCTT11.8867924528301887Illumina Single End PCR Primer 1 (95% over 23bp)
CTCGGGTGATCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTCTC11.8867924528301887No Hit
CGTTGATACCACCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC11.8867924528301887No Hit
CTCGTCCAGCGCGCCCTTCACCGTCTCGACGGCGTATCCCGCCTCGGACA11.8867924528301887No Hit
GTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAAA11.8867924528301887No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGATTTTAAACC11.8867924528301887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11.8867924528301887No Hit
AGCCCGTATTGTCCTTGCGCGGCCCCCGTAAGCCTTCCCAGATCTCGCCG11.8867924528301887No Hit
GTACAGTGCGCGCCAGCGGCGGCGGCTGAACCGGGGCCTGCGGCGGAAGC11.8867924528301887No Hit
AGCAAGAACACCTTGGCTGGCGAGCCAGGTTTCAGTAGCAGAGAGAAAGC11.8867924528301887No Hit
CCCTTTATTCAGCACCAGGAATACCCTTCGCACAGAACCAGCGAGCTTCA11.8867924528301887No Hit
GGTATCAACGCAGAGTACATGGGTCGCGCACCGAGATCTACACCTGTCTC11.8867924528301887No Hit
TTTTTGCGTTGATACCACCCCATGTACTCTGCGTTGATACCTGTCTCTTA11.8867924528301887No Hit
GTGGTGATCGGCGTGCTGGAAGAGAGTCCGCCGCTGCTGCTGCTTCCTGA11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11.8867924528301887No Hit
GTGGTATCAACGCAGAGTACATGGGTGAACGGCGCACCGAGTCTACACTC11.8867924528301887No Hit
AGCCGAACCTCGGGACGTCACTCCTGTTCTTCCCGGTCCTGTTCGCGATG11.8867924528301887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8867924528301887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.8867924528301887No Hit
CCCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGAT11.8867924528301887Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAA11.8867924528301887No Hit
TCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.8867924528301887No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA11.8867924528301887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTT11.8867924528301887No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.8867924528301887No Hit
GTATCAACGCAGAGTACATGGGCGACCACCGAGATCTACATCATCGGAAG11.8867924528301887No Hit
CTCGCCAACCGCGAGGGCGTGGAGATCCGGTACTACTCCGTGATCTACCA11.8867924528301887No Hit
TATCAACGCAGAGTACATGGGGCGACCACCGAGATCTACACCTGTCTCTT11.8867924528301887Illumina RNA PCR Primer (95% over 23bp)
GTACATGGGTGGCGACGGCAGCGAGGCGAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT11.8867924528301887No Hit
GATACCAATATCGCCTACCTCAAGGAGCACAAAGTACGCATCTGGGACGA11.8867924528301887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT11.8867924528301887No Hit
GCGGCGGCGTGAGCGTGCTGGCCGCCCTCTTCGACCTGCTGGACATCCGC11.8867924528301887No Hit
TCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGAAAAAAAAAAAAA11.8867924528301887Illumina Single End PCR Primer 1 (100% over 25bp)
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGTGTAGACTCTGTC11.8867924528301887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGATC11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)