FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA240.8595988538681949No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.7521489971346704No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA140.501432664756447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.42979942693409745No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA110.3939828080229226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.3939828080229226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.3223495702005731No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA90.3223495702005731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.3223495702005731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.28653295128939826No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA80.28653295128939826No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA80.28653295128939826No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.28653295128939826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA80.28653295128939826No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2507163323782235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA70.2507163323782235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.2507163323782235No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA70.2507163323782235No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.21489971346704873No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA60.21489971346704873No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA60.21489971346704873No Hit
CTCTAATTCCATTGTTTTTTTTAGATTCTCTCGGGCCTAGCCGTCCTTGG60.21489971346704873No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA50.17908309455587393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT50.17908309455587393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT50.17908309455587393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG50.17908309455587393No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14326647564469913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT40.14326647564469913No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACGAGATCA40.14326647564469913No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA40.14326647564469913No Hit
GTATCAACGCAGAGTACATGGGACGGTGGCTCAAGCCTGTAATCCCAGCA40.14326647564469913No Hit
CTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGT40.14326647564469913No Hit
GCGTGATCTCAGCTCACGGCAACCCCCTCCTTCCGGGTTCAAGGGATTCT40.14326647564469913No Hit
GTGTCCACTGACGCGCCTCACTTAACGCTGTTTTCCTCAGCGCCGGCCGC40.14326647564469913No Hit
ACGCTACCCTATGGCCGTGGGCCTCAACAAGGGCCACAAAGTGACCAAGA40.14326647564469913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT40.14326647564469913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.14326647564469913No Hit
GAAGAAACAAGAGGAGGGGCTGGGGAAGGGCTGGAAGGGCGGGCAGATGT40.14326647564469913No Hit
GTACATGGGTGGCCAACGTGCTGAAAACCCGTCTCTACTAAAATACAAAA40.14326647564469913No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA40.14326647564469913No Hit
GTCTCGCTCTGTCGCGCAGGCTGAAGTGCGGTGACGTGATCTCGACTCAC40.14326647564469913No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA40.14326647564469913No Hit
CCTCAAAGGAGCGGGGTCGCGAAGCTAGCTAGCAGCGGCCCCCCTCCAGG30.10744985673352436No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC30.10744985673352436No Hit
CTATAGCCTTGACCTCCCAGGCTCTAAGGATACTCCCCAGTAGCTGGGAT30.10744985673352436No Hit
GTACATGGGTGGCTCACACCTATGATCCCAGCACTTTGGGAAGCCAAGGT30.10744985673352436No Hit
CTCTATAGCTTTAATTGAGATGTAGGCTGGGTAGCCAAATCCTATCAGGT30.10744985673352436No Hit
CTGGTACCGAAGGCGGAGGTGGAGCCCGAGAGGGAACCAGCGGGGAAACT30.10744985673352436No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA30.10744985673352436No Hit
CAACGCAGAGTACATGGGTCTCTTCTCTTGAAGCAGATGCGAAGGCTCCC30.10744985673352436No Hit
GTCCACATCCCGGGAGAGAGAACGCAGGCGCCGAGAAAGGTCCAGGTCTC30.10744985673352436No Hit
GGAATATATAGATCGTTGGAGCGCAATGAAGTAGCCTTTGGAGAGAAGGG30.10744985673352436No Hit
CTATAGCTTTAATTGAGATGTAGGCTGGGTAGCCAAATCCTATCAGGTTG30.10744985673352436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT30.10744985673352436No Hit
GGCTTCAGAAAGCTAATTAAGTGCTCTGAAAAAGACACCGTTTCTTGAAC30.10744985673352436No Hit
GCCTGCCTCCTCCTCCCAAAGTGTTGGGATTACAGGTGTGAGCCACTGCA30.10744985673352436No Hit
GAGGAAAACTGCGACTGTTCTTTAACCAAACATCCGTGCGATTCGTGCCA30.10744985673352436No Hit
TCTCTTCCATGGCTGAGAGCACATCACTGGCATTCAGCGTCTTCCGCTTT30.10744985673352436No Hit
GGACTGCAGTGGTGTGATCATCGCTCATTGCAGCCTCAACCTGGGTTTCA30.10744985673352436No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCT30.10744985673352436Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAGAGTACATGGGTGGAAGGCTGAGGCACGAGAATCACTTG30.10744985673352436No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA30.10744985673352436No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG30.10744985673352436No Hit
CTTTTAATACTTTGGGGTGAGCCCCACAGGAATAAAAAACACTGGGAAGG30.10744985673352436No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAA30.10744985673352436No Hit
AATGTGAACCAACATTAACCATGAGCAATTTAAAAAAAAAAACAAAACAG30.10744985673352436No Hit
TAGTTTCTCCTTCAACACAAACTTAAAAACAAATGGCTGAACACGCAGGA30.10744985673352436No Hit
GGCCATGGGCTGGCTGATTTCAGTCAAAGGCCACACATTTGATCTTGAAG30.10744985673352436No Hit
GACAGGGATGTTTAAGTCTGCAGAAGTTTCTGCTACCTTTTCTTCAGCTA30.10744985673352436No Hit
GTATCAACGCAGAGTACATGGGATGATCGCGCCACCGAGATCTACACACC30.10744985673352436No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG30.10744985673352436No Hit
ACGCAGAGTACATGGGATCCCTGGATCAATTACTGGGGTAAGAGGTGGGA30.10744985673352436No Hit
ACCGTATACTACATACTACGGTGTTCCCTATGGACTGACGGCAGTACGAA30.10744985673352436No Hit
GCTTCGCATCTTGCTGCTGCCCCCGACTGACTGCGTAGTGCTCTCGCAAA30.10744985673352436No Hit
GTGCGATCTCGGCTCACTACAAGCTCCGCCTCCCGGGTTCACGCCATTCT30.10744985673352436No Hit
GTGCCGCACACCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAAAAT30.10744985673352436No Hit
CAAATAGGGAGAAGGCTTCCTTGAATTCAGCAATCTGTTCTTCGGTCAGC30.10744985673352436No Hit
CAGCGGGACTGCTAGGGACTGCGCGAGGTCCCGGCCGCGGGGCCAACTCG30.10744985673352436No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAA30.10744985673352436No Hit
GTACATGGGTCAGTAACTAGATGAGAGATCTGAAAAAAAATAATCCAGAA30.10744985673352436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT30.10744985673352436No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTACCAACGCAAAAAAAAAA30.10744985673352436No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA30.10744985673352436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT30.10744985673352436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT30.10744985673352436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA30.10744985673352436No Hit
GCCCAGGGATGCCCGGCGCAACTGGACGCTCATCGGGAGTCACGGGGTCG30.10744985673352436No Hit
GGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTGGCCGATCATCTCGGG30.10744985673352436No Hit
GGTAAGATACTTTTCTCATTTTGAATTCTTTCTTCATTTGGGCTCTGCCC30.10744985673352436No Hit
GGCGGGGACCGGACACCCGCTTCCCCGCCTCTTCACAACCAAGCCGCCGC30.10744985673352436No Hit
TCTCCTGCCTCAGCCTCCCGAGTAGCTGGAACTACAGGCGCCCAACACCA30.10744985673352436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA30.10744985673352436No Hit
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10744985673352436No Hit
GACAGAATCTCACTCTTATCACCCAGGCTGGAGTGCAGTGGCGCAATCTT30.10744985673352436No Hit
GAGTACATGGGTCCCTCCTCTGACTGTATCAGCGCTGCTCACACCGCGGG30.10744985673352436No Hit
GCTTCATCGGCTCCTCTCTATCGCGCCGAAGCGCTCGTCGCCTATGCGGA30.10744985673352436No Hit
CCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATAC30.10744985673352436No Hit
GAGATCTACACTTTTTTTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10744985673352436No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA30.10744985673352436No Hit
TCTCGACTCACTGCAAGCTCCGCCTCCCGGATTCACGCCATTCTCCTGCC30.10744985673352436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.10744985673352436No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA30.10744985673352436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)