FastQCFastQC Report
Tue 10 May 2022
EGAF00003658451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90974
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG4100.4506782157539517No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG3570.3924198122540506No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2990.32866533295227207No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA2530.27810143557499944No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT2480.272605359773122No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA2400.26381163849011807No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2250.24732341108448566No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA2170.23852968980148173No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT2070.22753753819772685No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA2040.22423989271660033No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA2040.22423989271660033No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1950.21434695627322092No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1940.21324774111284545No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA1920.21104931079209444No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT1910.20995009563171899No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1790.19675951370721306No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC1750.19236265306571107No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC1620.17807285598082967No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT1580.1736759953393277No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA1400.15389012245256886No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1390.15279090729219338No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1380.15169169213181788No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1310.14399718600918943No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA1310.14399718600918943No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT1290.14179875568843847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.13960032536768746No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC1260.13850111020731198No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1260.13850111020731198No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA1250.1374018950469365No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1230.1352034647261855No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1220.13410424956581No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT1220.13410424956581No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG1220.13410424956581No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG1190.13080660408468353No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT1140.12531052828280606No Hit
ATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAG1140.12531052828280606No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC1130.1242113131224306No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG1100.1209136676413041No Hit
GTACATGGGCATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTG1100.1209136676413041No Hit
CTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATT1090.11981445248092862No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT1090.11981445248092862No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1090.11981445248092862No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC1080.11871523732055313No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1070.11761602216017764No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC1040.11431837667905116No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC1020.11211994635830017No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG1000.1099215160375492No Hit
TAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCC990.10882230087717369No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT980.1077230857167982No Hit
ATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATG960.10552465539604722No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT960.10552465539604722No Hit
GTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTC950.10442544023567173No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG950.10442544023567173No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC950.10442544023567173No Hit
TTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC940.10332622507529624No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG940.10332622507529624No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT940.10332622507529624No Hit
AACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGT920.10112779475454525No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA920.10112779475454525No Hit
ATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATG920.10112779475454525No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG910.10002857959416976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)