FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGAATCACGAG38.108108108108109No Hit
GTGCTTATTTTATTTCACCAACAAGGAATCCAGGGCACAGACAAGTTAAG25.405405405405405No Hit
CGTCCTGGATGTCGAGCAGCGACTGCAGCCATTTGGTGCCCAGGCGGTCG25.405405405405405No Hit
GCACAAAGGCCCGGTCGCTTTTGCCCATCGGCCCGTCGTATCGGCGCGAT25.405405405405405No Hit
GGCCATCGCCAATTGAGAGTCCAGGTTGCTTTCAACAATTCCAGAGCTTC12.7027027027027026No Hit
AAAAAGGGGTTTCTTTTTGTCTTTCTGTAAGGTGGACTTCCAGCTTTTGA12.7027027027027026No Hit
GCACTTTGGGAGGCTGAGGCAGGTGAATCACTTGAGGTCAGGAGTTTGAG12.7027027027027026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT12.7027027027027026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGCCACATGAAGCTGCACTCCAACGCAAACCCTTGCAATACCAGGCCT12.7027027027027026No Hit
CCGCCAGCCCCAGCATGGCGCGGAAGGGGAACTTGGACTGTCCCGCCGCC12.7027027027027026No Hit
GTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCG12.7027027027027026No Hit
ATTTTATCCTGGATTTGAAATATTTCAGGAAATACATCATTTGCCTTTTA12.7027027027027026No Hit
GGTAACGGCGGCTGGTGCCTTTAAATGAGCCGGGGCTGGCCGGGCCGGAG12.7027027027027026No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG12.7027027027027026No Hit
CATAGAAAGACCCCGTCTCTACAAAAAATAAAAACGAATTAGCTGGGCAT12.7027027027027026No Hit
GTCTTGTGCATCAGGATGAAGGTTGCGGCGGTGATCGGCCACGAAGCTTC12.7027027027027026No Hit
GGCTACTACCGTCCTGACTTCTGATATCATAGATGAGGTTTGGATGGCAA12.7027027027027026No Hit
CTTCTCAACAATTTCTCACTTCATTGATCATTTCTAGTTGGAGACACTTT12.7027027027027026No Hit
AGTAAGGATGTGCCTGATGAGGAACTGTTTTTAGCAACTGATGATGAAAC12.7027027027027026No Hit
GAAATACCCATTCATGACAGAACTTTTCTATTATTAGTACAAGCTGTCTC12.7027027027027026No Hit
ACGCCGATGAGCGCGCCCGCGGGGGCCTCGCTGATGATCGCGGGGAAGGC12.7027027027027026No Hit
CCCTCATTTTCTTCATCACTTTGGGAAATAAATCTTGAATTGTTGTTGTG12.7027027027027026No Hit
GCGCGGATGCGCGCTTCCAGTTCGTCGAGGTCGAAGGGCTTGGGCAGGTA12.7027027027027026No Hit
AAGGAGGTGCACGCCCTCAAGCGGGTCACCTTCGAGGTGCCCAACGGCAC12.7027027027027026No Hit
GAAAGTTCAGAATTTTATTAATGAGTTGCTGAAACTTTAAAGATAGTTTT12.7027027027027026No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGAT12.7027027027027026No Hit
CAAAAAACCACCTCCTGGATTCATTAATTTTTTGAAGGGTTTTTTTGTGT12.7027027027027026No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTA12.7027027027027026Clontech SMART CDS Primer II A (96% over 26bp)
GGACTGGTGGAGAGGATGGATGGGCATTAGCAAAACACCTAAGTAGCAGC12.7027027027027026No Hit
GTCCAAAACCGAACCTATAAAAAGAGATAAAAACTTTTTCGTAGCAATTT12.7027027027027026No Hit
GAATTATTCTCCTCAATTTCAAGGCTTACATAACTATATTATTCAAGACT12.7027027027027026No Hit
CTATTATATCACAGGTGAGACCAAGGACCAGGTAGCTAACTCAGCCTTTG12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)