FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCACCCAGGCTGGAGTGCGGTGGCGCGATCTCGGCTCACTGCAAGCT38.108108108108109No Hit
CTGGTGGCGTTGCTGGCGGTGTTCAGCGAATACGCCGGCGTGCTGGGCCC25.405405405405405No Hit
TCACAAAGCCTGAGCTCCATGGAGCCAAGGGCTCTGTCTGCATCCGCAGC25.405405405405405No Hit
CTCGGCATCCTGCACCAGATGTACAAGCCCCTGCCGGGCAAGTTCAAGGA25.405405405405405No Hit
TTGCCCAGGCTGGTCTCAAACTCCTGACCTCAAGTGATTCACCTGCCTCA12.7027027027027026No Hit
ACCTTGGTTAAACCCAGCCCTCTGCCTACTCTACACCTTCTCCCATGCAG12.7027027027027026No Hit
CCCTAGGACTTTCAATCAAAAGCTGGAAGTCCACCTTACAGAAAGACAAA12.7027027027027026No Hit
GCTAAAATACTTACATAATCAAGGCCAACGCAGCAGAAACAAGTGACAAA12.7027027027027026No Hit
GTGCAATCCTCGGGCAGAGGGAATAAATTGCTACGAAAAAGTTTTTATCT12.7027027027027026No Hit
GTCACGAGGTAATTTGGGAAAACATCATCTCTTCAAAAAGACAAAGGGAA12.7027027027027026No Hit
GGTGCATGCCTGTAGTCCCAGTCACTTGGCTCTGAGTGTGGGCTCTTCTG12.7027027027027026No Hit
GGGCTACTACGTGATCCTGACCGACCAGCCGGGCGAAGCTTCGTGGCCGA12.7027027027027026No Hit
TGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCG12.7027027027027026No Hit
TCATAGCTCACTGCAGCCTCGACCTGAGTAGCTGGGACCATAGGCACACC12.7027027027027026No Hit
ATACAGCATGGAGATGTCACCAATCGGTTTGACACAACCACCTTTTCAAC12.7027027027027026No Hit
GCTTTGGAAGGCTCTTCAGCCTCAGCTTCTCAAAGCAGCTCAGAGATCGA12.7027027027027026No Hit
CCACAATCACCACGACGATGAGGACCACCACCACCACCACGATAAGAAGG12.7027027027027026No Hit
CTCGGGCGCCGCCGCATCGACGTGCGCGTCTGCGGCGACGGCGCCCAGGC12.7027027027027026No Hit
GGGTGCTGGCCTTGCCCCATACCTCGATGGTTCCGGCGGTGGGCGGCAGG12.7027027027027026No Hit
GCTCCGGCCCGGCCAGCCCCGGCTCATTTAAAGGCACCAGCCGCCGTTAC12.7027027027027026No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTA12.7027027027027026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGCCTGGCCTTCATCGCCTTCCCCGCGATCATCAGCGAGGCCCCCGCGG12.7027027027027026No Hit
AATTTATTCATTTCACAAATGTAGAACCTTTCTCTGTTAGACATTTTTCT12.7027027027027026No Hit
GTTCTCCAGGCAAAGTCAAGGAGCGGGGCCAGATACGCAGAGATGTCTTC12.7027027027027026No Hit
TTATACTTCTACATTGCTTATAGTAAAGTTAAAAGGCAAATGATGTATTT12.7027027027027026No Hit
ATATTACTCTGCTACAAAGTGTCTCCAACTAGAAATGATCAATGAAGTGA12.7027027027027026No Hit
CCACAAGAGAAAGCAGGGAAGATCCAAAATTGACACCCTAACATCACAAT12.7027027027027026No Hit
GGAAGTTTCTCAAGTTGTAAAATCAGGTTTTGATTTGGCATTCATTGCCT12.7027027027027026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
GACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCA12.7027027027027026No Hit
CTTGTACTAATAATAGAAAAGTTCTGTCATGAATGGGTATTTCCTGTCTC12.7027027027027026No Hit
GATCCGCCGCTCCGAGGACCGCTCACCCTACATGCGCGGCATGATCAGCG12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)