FastQCFastQC Report
Tue 10 May 2022
EGAF00003658785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40430
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG3320.8211723967350978No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT2080.5144694533762058No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT2050.5070492208755873No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC1960.48478852337373235No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT1870.4625278258718773No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC1810.4476873608706406No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA1780.44026712837002224No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT1730.427900074202325No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC1610.3982191441998516No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT1580.3907989116992332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.3833786791986149No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA1450.3586445708632204No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG1320.3264902300272075No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC1230.30422953252535245No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT1220.301756121691813No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT980.24239426168686617No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT960.2374474400197873No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT930.2300272075191689No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT910.22508038585209003No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT910.22508038585209003No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG810.2003462775166955No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG790.1953994558496166No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA770.19045263418253772No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA740.18303240168191937No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC730.1805589908483799No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT710.17561216918130101No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA690.1706653475142221No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC690.1706653475142221No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA690.1706653475142221No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG690.1706653475142221No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT680.16819193668068266No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG660.16324511501360375No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG640.15829829334652487No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG630.1558248825129854No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA620.15335147167944596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.15335147167944596No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG600.14840465001236705No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC590.1459312391788276No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC590.1459312391788276No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG580.14345782834528814No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC580.14345782834528814No Hit
GAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATC570.1409844175117487No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC560.13851100667820926No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC550.1360375958446698No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC550.1360375958446698No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA530.1310907741775909No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC490.12119713084343309No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT480.11872372000989365No Hit
CCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACCGCTCCAAGCC480.11872372000989365No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT470.11625030917635419No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC460.11377689834281475No Hit
CCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGC450.1113034875092753No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA450.1113034875092753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.1113034875092753No Hit
GTACATGGGACGCCGTCCGGGTGGGCCTAGCAGTCGCTCCATTTATCGCT450.1113034875092753No Hit
GTACATGGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGT450.1113034875092753No Hit
CCTCCCTACCGCTCCAAGCCCAGCCCTCAGCCATGGCATGCCCCCTGGAT430.10635666584219639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA420.10388325500865693No Hit
ACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGC410.10140984417511749No Hit
AAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGG410.10140984417511749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)