FastQCFastQC Report
Tue 10 May 2022
EGAF00003658786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40430
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG3370.8335394509027949No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC2030.5021023992085085No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT2030.5021023992085085No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT1850.45758100420479847No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA1850.45758100420479847No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT1830.45263418253771953No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT1810.4476873608706406No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA1770.4377937175364828No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT1620.400692555033391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.3907989116992332No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC1570.3883255008656938No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC1510.3734850358644571No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT1250.30917635419243134No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT1200.2968093000247341No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG1170.28938906752411575No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT1090.26960178085580017No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC1030.2547613158545634No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT1020.252287905021024No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT970.23992085085332676No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG920.2275537966856295No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT900.2226069750185506No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC880.2176601533514717No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT820.20281968835023498No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT810.2003462775166955No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG800.19787286668315607No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA660.16324511501360375No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG640.15829829334652487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1558248825129854No Hit
GTACATGGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGT620.15335147167944596No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC600.14840465001236705No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA600.14840465001236705No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC590.1459312391788276No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG580.14345782834528814No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC570.1409844175117487No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG570.1409844175117487No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA570.1409844175117487No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG560.13851100667820926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.13851100667820926No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC560.13851100667820926No Hit
ATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTC550.1360375958446698No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA540.13356418501113035No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA530.1310907741775909No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA520.12861736334405144No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC520.12861736334405144No Hit
TTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTAC510.126143952510512No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC510.126143952510512No Hit
CCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACCGCTCCAAGCC510.126143952510512No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG480.11872372000989365No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG470.11625030917635419No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG460.11377689834281475No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC460.11377689834281475No Hit
TATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCATCAG460.11377689834281475No Hit
AAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGG460.11377689834281475No Hit
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC450.1113034875092753No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC440.10883007667573585No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC440.10883007667573585No Hit
GTACATGGGACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGA430.10635666584219639No Hit
GTACATGGGACGCCGTCCGGGTGGGCCTAGCAGTCGCTCCATTTATCGCT420.10388325500865693No Hit
GCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAA420.10388325500865693No Hit
CCTCCCTACCGCTCCAAGCCCAGCCCTCAGCCATGGCATGCCCCCTGGAT410.10140984417511749No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT410.10140984417511749No Hit
CGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCG410.10140984417511749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)