FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTGCTCGAGGCCCTGCGGGACCGCGCCGGCGATGCCCCGGATCTGCG42.941176470588235No Hit
GGTTAACAGTTCATAAAATAACAACTTTTTAAAAAGTCGCTAAGGAAAAA42.941176470588235No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.2058823529411766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT32.2058823529411766No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.2058823529411766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACGGAGGTCTGCGTCCTCTACCAGCGCCGCTTCGAGGCGGCGACCGAC21.4705882352941175No Hit
CCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCT21.4705882352941175No Hit
CTTCATAGAAGCTTTTAAACCAACGCGTTGAGGCATGAGATCCAGATGTG21.4705882352941175No Hit
CTCTTTCCCTACACGCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAG21.4705882352941175No Hit
TCTCATGATTTCTGATTTTGGTGGTATAATTGTGCATGGATGATTTCGTA21.4705882352941175No Hit
CCAGTAGCCCTGATGCTCAGATCTTTCAAATTCTCTTCTTCACCAAAAGA21.4705882352941175No Hit
GGATAAAAAGACTACCCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.4705882352941175No Hit
CTGCTATACAGACTGCTGCGGCATGCGAGGATGGGAAGTAATGTGAGGTT21.4705882352941175No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATAC21.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGACCCGCCGGGCAGCTTCCGTGGGTGGTGGTGCATGGCCGTTCTT21.4705882352941175No Hit
ACGTGGAACTGTGAGAAAATTAAACCTCTTTCCTTTGTAAGTTACCTAGT21.4705882352941175No Hit
GAGTAGACCTCACTCGCTCTTTGGCTCGCTCCTAGCTTTTCTTTTGCAGC21.4705882352941175No Hit
TTGATACCTCGGCCAGAATCTGTGCCCCTGAGTTGCCGCTGCCCTGTCTC21.4705882352941175No Hit
CTCCCAACCCCAGCCCTGATCATATTACTCACCAGTTCTCAAGTTAGAAA21.4705882352941175No Hit
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT21.4705882352941175Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.4705882352941175Clontech Universal Primer Mix Long (95% over 21bp)
ACCGTGGACGGCGCGCGTGTCGAGGTCGGTGCCGATCGCTTCATGCGTGA21.4705882352941175No Hit
GCCGTCATGCGTCCCACTGAGGCTGTTGTCTCTCGGTCGGCAGAACCTCG10.7352941176470588No Hit
GCTGGACGGTGCGCGAGCTCGCGCCGCCGTGGTGGGTCGCAATGGGACCT10.7352941176470588No Hit
TGGGTGATACGCGCCACCGAGATCTACACTCTTTCCCTACACGACGCTCT10.7352941176470588Illumina Single End PCR Primer 1 (97% over 38bp)
GTTACAGGCATGAGCCACCAGGCCCAGCCCGGGATTAGCTGTCTCTTATA10.7352941176470588No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGTACATGGGAAGC10.7352941176470588Clontech Universal Primer Mix Long (95% over 22bp)
GATCACCACGGTCAGGGCGGTCCGCGCGCTGTCTCTTATACACATCTCCG10.7352941176470588No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG10.7352941176470588No Hit
GGAATACATCCTGCTCCTGGCCGATGACCGCACCGAAATCACCCGCACCG10.7352941176470588No Hit
CATGGGAGTGTAACGGTGGTCCGTTCATTCCCATGTACTCTGCGTTGATA10.7352941176470588No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT10.7352941176470588No Hit
CCGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
CTTCTGGCTCCTGTTTTGTCTTGTGAGGCCCTTCTTTTATTGGCCTAACG10.7352941176470588No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGATTCCATGTACTCTG10.7352941176470588Illumina Single End PCR Primer 1 (97% over 35bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATAC10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGTGAAGGTGCGAAGACCTTCATGAAGCGTCACCTCGGAAAGAACAAGG10.7352941176470588No Hit
GAACGGGGAGGTGAAGGTCAGGTCTTCCACCTTGACGGTATAGATGTCCA10.7352941176470588No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAACCCTAAAGCCAATTGTCAAGCATTTACCAGCAAACCACTGATTA10.7352941176470588No Hit
GTGGTCGCCGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTTCTATTTGCATCTCGTATG10.7352941176470588No Hit
GTGTAGGGAAAGAGTGTAGATCGTGTCCCGACCCATGTACTCTGCGTTGA10.7352941176470588Illumina Single End PCR Primer 1 (95% over 22bp)
TAACAATGTTGATGAAAACAGAAAATTAGACTTACAGTTCTAGAATGAAA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAATGCGGTTATAACCAG10.7352941176470588No Hit
GAGTACTGGTGGCCATCGACGGTTGTGAGGGCGCGGTTTGGCCAATACCA10.7352941176470588No Hit
CCGCGGCGGCGGCGGCGTCTGGATCCGGAGTCCAGTTTGGGGTGGCGTGG10.7352941176470588No Hit
ATACGCCACAGTGGATACGATCTCCGCCATTCAGCCCCCCTTCACGCCAA10.7352941176470588No Hit
GCTGAATATGTGGGCATTGTATATTTTCGGGAGTGTTGCTGAACCACTTT10.7352941176470588No Hit
GAATATCGCTGCTGGTTCTGCCCAGTGCCCGGCTTCTCACCTCAGATGTT10.7352941176470588No Hit
TCTACACTCTTTCCTACACGACGCTCTTCCGATCTCATGACAGCAACTCC10.7352941176470588Illumina Single End PCR Primer 1 (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTCTTCAGTGAAAGTGTTCTCTCCCTTTGGCCATCTGGGAAGAAAGC10.7352941176470588No Hit
GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCACCCATGTA10.7352941176470588Illumina Single End PCR Primer 1 (97% over 38bp)
GGCCGAACCATCAATCACCTGACCATCAGCAGGCACAGAAGCACCGGGAC10.7352941176470588No Hit
ACAAGCCTGAGATCCGTCACGCGCAGAGCGGAACGGCCGTAGCGAACTTC10.7352941176470588No Hit
CCTTTGTTCTGAAGACTTTTGCCCAAGCTCGAGCCTACATCTTCATCGAT10.7352941176470588No Hit
TTGCAATTTCGGTACAAAGCGTGGGGGTTTTTGCGTTAATTCAGGCGGGC10.7352941176470588No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCCTGTACCTGTCTCTTAT10.7352941176470588Clontech Universal Primer Mix Long (96% over 28bp)
CCACCAGCCTGGCCTTGTTGGTCTGCAGGTGGCCGATGACCGACCAGCGC10.7352941176470588No Hit
GTCACGCACCGCACGTTCGTGGGGGACCAGGGGCTAAAACAATAGGATAA10.7352941176470588No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA10.7352941176470588No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.7352941176470588Clontech SMART CDS Primer II A (100% over 26bp)
ACCATGTAGAGGAAGGTTGACATGAAAGGGGTACAAGGGTGCTGGAAATG10.7352941176470588No Hit
CCCATCTTGTTACACCTATACTTCCATCCACTCCCCCGCCCTGCTGTCCA10.7352941176470588No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT10.7352941176470588Clontech SMART CDS Primer II A (96% over 25bp)
ATTCGACATCCTTGCAATTGGTTCCAACACCTCACGAGCGTGCTCCGGAC10.7352941176470588No Hit
CTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAACAG10.7352941176470588Illumina Single End PCR Primer 1 (100% over 22bp)
GGCCAATTCCTACACGACGCTCTTCCGATCTCAGCAACTCCAGTCCTTGC10.7352941176470588Illumina Single End PCR Primer 1 (100% over 23bp)
GGGTGTGTGGCGACGCGGGGATCGACATCCCGTGATTGTGGGCGGCCATG10.7352941176470588No Hit
GGCCAATGTGCTGTTCTTCGACAAGAAGCCGGCGAGCCAGACACCGTGGA10.7352941176470588No Hit
GCGTTCAACCAGACCATCATGGACTCGCTGACACGCTACCACCGCATGAA10.7352941176470588No Hit
ACGCTGTGGTGGCGGTTGCGCATGGCGGTGCTGCGTTTGATGAACCGGCA10.7352941176470588No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC10.7352941176470588No Hit
GTGCTGCGGGGCGTGTTGCACCCGCGGCACAGCGTGTTTGACTCGTCCGG10.7352941176470588No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA10.7352941176470588No Hit
GTACATATTGAACAATTCATTTGGTGGCTAGAAAATAGGGCGTCTGTGGA10.7352941176470588No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAAACCCCGA10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTTGTCAGGACATAGGATTTCCTCTTGCTCATCAAAGTCACGAGGTTC10.7352941176470588No Hit
GTACATGGGAAGCAGTGGTATCAACGTCGAGTACATGGGAAGCAGTGGTA10.7352941176470588No Hit
TCCCTAGAGTAGACCCGTTCAATTTCACATGACAGCACCATTGTGCCATC10.7352941176470588No Hit
CGACGAAACAGCGCGAGAACAGCGTGCCCGCCTCGAAAAACATTTCCCGG10.7352941176470588No Hit
GGGTGACCGCGTTCGGATCATGGAGACCCGTCCGCTGTCGGCCACCAAGC10.7352941176470588No Hit
CTGGTGTTCTCCACCCTTTCGGTGTGGTTGCGCGAGGCGGGTGTTTCCCG10.7352941176470588No Hit
ATATAGTCTCCATTGGCAACTTTTCATGTTGCCAATGGAGACTATATTAT10.7352941176470588No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
CATCAGTAGTATCGGTGGGTTTGAATCGTTGCTGTAGGAATAGAAGAGAA10.7352941176470588No Hit
GTCTTAATTTCTGCAAGTCCTGCAAAGTGGGAAAATAAAAATAAGCTAAT10.7352941176470588No Hit
CAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCC10.7352941176470588No Hit
AGGCTGGTTAGATTGGCAGGAAGCGGCACGAAAACTGGTAAAATCATGAG10.7352941176470588No Hit
GCTCTATAGTCAATGGGGTCACACAGGGTAACATGGATCTGTTCGTTTTG10.7352941176470588No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACAT10.7352941176470588No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATACA10.7352941176470588No Hit
TCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCG10.7352941176470588No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAATACTTTTT10.7352941176470588No Hit
GTCCTGGATGCGTCAGAAAAAAGATGTATACGACCAGCTGAGTCAAACGA10.7352941176470588No Hit
CATTTTATTAGGGGCTTAGAAAGGAGTCCTAGGGGATAATTCTGTTTTCC10.7352941176470588No Hit
ATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG10.7352941176470588No Hit
GCATGTTTCTGTGCATGTGTGTGCTTATGTGTGCCTTGTGCAGGTGCTGT10.7352941176470588No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG10.7352941176470588No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGGTATCAACGCAGAGTACATGG10.7352941176470588No Hit
TTGCACAGCATTGGCTTGCATAGCATTGCATAGCATAGACTTGCATTGCA10.7352941176470588No Hit
TTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTA10.7352941176470588Illumina Single End PCR Primer 1 (100% over 26bp)
CTATCATGGAAAAGTTATCCCACAGTTTAGATAACTTGGAAGATAACTTC10.7352941176470588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)