FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGTGCAGGGGCGCTTGCACACCGACAGGCACTCCACCGGCTCGACC42.941176470588235No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.2058823529411766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTGTTTAATCTGAAGTATTTTTTTTAAGCTCTGTGCAAACTTCTTAT32.2058823529411766No Hit
TGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGT32.2058823529411766No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32.2058823529411766Clontech Universal Primer Mix Long (96% over 26bp)
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT21.4705882352941175No Hit
GCTGCAAAAGAAAAGCTAGGAGCGAGCCAAAGAGCGAGTGAGGTCTACTC21.4705882352941175No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA21.4705882352941175No Hit
CATCGCCACCTTCAGGCCGAGCTGGTGCAACGCGGCAATGGCCGCGGGCG21.4705882352941175No Hit
CCCATGTACTCTGGGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.4705882352941175Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTTTATGGAGCAAGATGTTCAGGATCAGCTATAACTTACCTGAGCTGGA21.4705882352941175No Hit
GGTTTTAGAAGAAAGGAATGGTTAAATGTTTTCTCGTGACAGCGTAGTGG21.4705882352941175No Hit
GGCAGCGGCAACTCAGGGGCACAGATTCTGGCCGAGGTATCAACTGTCTC21.4705882352941175No Hit
TGATAACTCTGGCATAGTTTTCTAACTTGAGAACTGGTGAGTAATATGAT21.4705882352941175No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATAC21.4705882352941175Clontech Universal Primer Mix Long (96% over 28bp)
AAAGACATACCTGAGACTAGGTAACTTACAAAGGAAAGAGGTTTAATTTT21.4705882352941175No Hit
TATATACAGTAAAGATACGAAATCATCCATGCACAATTATACCACCAAAA21.4705882352941175No Hit
AACCTCCTCACCCACGGTGATCGCGCGGCGCCAACGATCAGACATATAAC21.4705882352941175No Hit
GTGCCGCAGCCACTGCTGGGCATCGAGAATGCCCGCGTCGGTCGCCGCCT21.4705882352941175No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGTATCAACGCAGAGTACATG21.4705882352941175Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.4705882352941175No Hit
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT10.7352941176470588No Hit
CCGCTGCCTTAACCGAGAATACTGCGGCTGTGGTGCGTCACTTCGTTGAC10.7352941176470588No Hit
TATCAACGCAGAGTACATGGGTGATCGGCGACCACCGAGATCTACACACT10.7352941176470588Illumina Single End PCR Primer 1 (95% over 23bp)
GCAAGCAGCATGCAAGCACTTACTTGCCATGCCCACTATAATAGACGGTG10.7352941176470588No Hit
GTCCCATTGCGACCCACCACGGCGGCGCGAGCTCGCGCACCGTCCAGCCT10.7352941176470588No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCAGAAGGGCGATGGTGATATAGGCGGAGAGGGTCACTTCATCTTCTA10.7352941176470588No Hit
ATACAAGGGAGATCCTCCTACAGAACAAGTTCAAGAGGAACCTCGTGACT10.7352941176470588No Hit
ACAGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CCTTGTGCATCCGCTTGGCGAAGCGGCGGACCGCTTCCTCGTCATCCGGG10.7352941176470588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7352941176470588No Hit
TATCAACGCAGAGTACATGGAATCGGCGACCACCCAGAACTAAACACTTT10.7352941176470588No Hit
GGTATCAACGCAGAGTACATGGGAAGGCCAATTCCTACACGACGCTCTTC10.7352941176470588No Hit
CACCTGCACAAGGCACACATAAGCACACACATGCACAGAAACATGCCTGT10.7352941176470588No Hit
CTTCGCCCCTTTTGGCGGAGCGAAAAGCCGGTAAGGTCTTTGGCGTGAAG10.7352941176470588No Hit
TCTTCCGTTAGGCCAATAAAAGAAGGGCCTCACAAGACAAAACAGGAGCC10.7352941176470588No Hit
GATGGTTGGATGGACTTTTGGTTGGATACATTGGAAATCCAGGGTGTGTC10.7352941176470588No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAAA10.7352941176470588No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAA10.7352941176470588No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCT10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC10.7352941176470588No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
TATTTGAAGTGTTAAGTACTCTCAGCACCTACCTTAACAATGCATCATAG10.7352941176470588No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT10.7352941176470588No Hit
CAGTGTACGTTGTCTGCTTGCCCACAAGAGGCCGGCTACACACGTATTCA10.7352941176470588No Hit
GAGTACATGGGATTACGGCGACCACCGAGATCTCACTCTTTCCCTACACG10.7352941176470588Illumina Single End PCR Primer 1 (95% over 21bp)
TCCCTACACGACGCTCTCCCGGGTCTTGTCGATACCAGTACAGATCGGGA10.7352941176470588Illumina Single End PCR Primer 1 (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GTGATCGTGCGTCCCGGTGCTTCTGTGCCTGCTGATGGTCAGGTGATTGA10.7352941176470588No Hit
TGACAGTTGTATAGCCACCACCCAAAACAAGAGATGGAGGACATTTCCAG10.7352941176470588No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGACGGCGACC10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTCCGTCGCGACGCACCTCGCCGCAGAAACCGCACACGAGCGTCCCC10.7352941176470588No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC10.7352941176470588No Hit
CAATTTAAGAAATGGTTCAAAACTGGATTCATTTAAATTTAAAAACTTTA10.7352941176470588No Hit
GTTTCCGCCCTTGTCATTCCACGTTTCCGACGTAGCTACGTTGAAGTTCG10.7352941176470588No Hit
CCCTTGCAGTGACTGGCTGAGCAATTGGGTTGAGAATAAAATAAAAATGC10.7352941176470588No Hit
ACTCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTCTGCGTTG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ATGCTGAAGACATTTAAAAGTCAAATACCTGAACAAGAATGCAGCTCCTC10.7352941176470588No Hit
GCCTCGATGACCTCTTGATCGCCGACCGTGCCAAGCAAACGACGATGCTC10.7352941176470588No Hit
CCCGATGGCCTCATCTTCCCAGACCGGGCCACGCTGTATGTGACGGCCAT10.7352941176470588No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC10.7352941176470588No Hit
GCCCTGTTTAATCTGAAGTATTTTTTTTAAGCTCTGTGCAAACCTCTTAT10.7352941176470588No Hit
GTGTAGGGAAAGAGTGATCTCGGTGGTCGCCGTCTCCATGTACTCTGCGT10.7352941176470588Illumina RNA PCR Primer (95% over 21bp)
TTAGAGGATTCGGTTGGTTAACAAAAGAAGGAGCCCAGGAGAACAGTGCT10.7352941176470588No Hit
CCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC10.7352941176470588No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC10.7352941176470588No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTATTATGGTTTTTAA10.7352941176470588No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GTTGACGTGCACCGGAGGTCCACGCCCGGTGGCGGTCGATGACCTCTGCG10.7352941176470588No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
AGACACATACGCACCTCAAAACGAACAGATCCATGTTACCCTGTGTGACC10.7352941176470588No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7352941176470588Clontech Universal Primer Mix Long (95% over 22bp)
TTATGACACAGTGTTAAAGCCCTCACCAGAAGCCAAGACCATGCCCTTGA10.7352941176470588No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT10.7352941176470588Clontech Universal Primer Mix Long (96% over 28bp)
CATTAGCAGCATATGCTGTTCGCTGCCGCAAAATTGGAGTTGGTTAGCAG10.7352941176470588No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATTACGGCG10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
ACCCATCACAGCACCAGATGCACCGGCACTGATATGTGGCAGTAATTTGG10.7352941176470588No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCCTTTT10.7352941176470588Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAGAGCGACGAGATCTACACTCTTCCCAT10.7352941176470588No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATAC10.7352941176470588Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTG10.7352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCAC10.7352941176470588No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTA10.7352941176470588No Hit
CCTGCCGACAGGGGCGCATCCGCGCCCTCGGTTTCGACGGCTTGCGTCAG10.7352941176470588No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATACA10.7352941176470588No Hit
TCCCCACACCACACAGCTCTCCCCCGCTGGGCAGGATTCCTCGGAGAACG10.7352941176470588No Hit
CGCGCTGACCGCCCTGACCGTGGTGATCCTGTCTCTTATACACATCTGAC10.7352941176470588No Hit
CTACTAGGTAGTCATTGAGACTTGAAAACTCAATGCAGAAAGGTTTACTT10.7352941176470588No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGATGCGTTTTAAC10.7352941176470588No Hit
TCCCACGCCACCCCAAACTGGACTCCGGATCCAGACGCCGCCGCCGCCGC10.7352941176470588No Hit
GTGCAGGAACGCATTGCAGCGGCCTGCCAGCGCGCGGGGCGTGATCCAGC10.7352941176470588No Hit
CAACGCACCTCGTCGCGATCGGTATCGACAAGACCAATTTGCCGACCCGG10.7352941176470588No Hit
GGGCAGACGTTCAGGTCGTCAATATCCAGAACCGGTAACGAACCATAACG10.7352941176470588No Hit
GCCCCGCTTAATGTCCAACTGATGCAGCAGGCAGCGACCTATCTGGAAGG10.7352941176470588No Hit
AGCGATGCCTGCGTGGTCGGTTCCGGGAATCCAGGCCGTGTTGTAGCCCT10.7352941176470588No Hit
GTGATGGTGATGGCCGCCTGGTCACCGAGTGCCAGAGCGACATCCATGCC10.7352941176470588No Hit
AGCGTATATTAAAGTTGCAGCAGTTAAAAAGCTCGTAGTTGGATCTTGGG10.7352941176470588No Hit
CCTTCGGGTTGCTCCGCGCTCCAGGTGGGCGCGCGGTCGTGCCGGTTGGC10.7352941176470588No Hit
CATGACTAGGCACAGACTAACTCTTTCCCTACACGACGCAGCTCCGTCGT10.7352941176470588Illumina Single End PCR Primer 1 (95% over 21bp)
GGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGG10.7352941176470588No Hit
CTACTCCTCCGTTCTCACCTCCCACTTTCTAAAACCTGGCCATTATTTCA10.7352941176470588No Hit
CTAATCCCGGGCTGGGCCTGGTGGCTCATGCCTGTAACCTGTCTCTTATA10.7352941176470588No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGA10.7352941176470588No Hit
AAAGAATGTGAACTGCCTAACTTACATCTAAAGATACACGTATTCTAGAC10.7352941176470588No Hit
GGGTTACACAGCGCCATGCCGATCAGGCCGACGGCGATCAGCGCCTGGGA10.7352941176470588No Hit
TGGTGGGGTCATAAGAGATGGCACAATGGTGCTGTCATGTGAAATTGAAC10.7352941176470588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)