FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT873.0537030537030536Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA873.0537030537030536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG301.053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC301.053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC270.9477009477009477Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG260.9126009126009126Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG230.8073008073008073Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT210.7371007371007371Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.666900666900667Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA180.6318006318006317Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC170.5967005967005967Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC170.5967005967005967Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.5616005616005616No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG150.5265005265005265Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA130.4563004563004563Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA120.4212004212004212Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG120.4212004212004212Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA120.4212004212004212No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG120.4212004212004212Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT110.3861003861003861Clontech Universal Primer Mix Long (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG110.3861003861003861Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT100.35100035100035104Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA100.35100035100035104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG100.35100035100035104Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90.31590031590031586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC90.31590031590031586Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT90.31590031590031586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA90.31590031590031586Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2457002457002457Clontech Universal Primer Mix Long (95% over 22bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA70.2457002457002457Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC70.2457002457002457Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60.2106002106002106Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA60.2106002106002106Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60.2106002106002106Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA60.2106002106002106Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50.17550017550017552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT50.17550017550017552No Hit
GGGCTTCCAAACCTGATCTCACTCTCAACAGGCGATGGTGCTGATGTTTC50.17550017550017552No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC50.17550017550017552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC50.17550017550017552Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA50.17550017550017552Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT50.17550017550017552Clontech SMART CDS Primer II A (100% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50.17550017550017552Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC50.17550017550017552Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.17550017550017552Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG50.17550017550017552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA40.1404001404001404Clontech SMART CDS Primer II A (100% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGA40.1404001404001404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA40.1404001404001404Clontech Universal Primer Mix Long (96% over 25bp)
CTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGGCT40.1404001404001404No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT40.1404001404001404No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC40.1404001404001404No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATG40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1404001404001404Clontech SMART CDS Primer II A (100% over 21bp)
GCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGCCAAGTGTGCC40.1404001404001404No Hit
TTTCTGCTCAGGAAGTCTCTTCACTCCTAAGCTTCATATGTTGTCCTTCT40.1404001404001404No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1404001404001404Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.1404001404001404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1404001404001404No Hit
GCTTATTGTTTTTCCTGTTCAGTAGATTGGAAATGTCATAACACTTACCT30.1053001053001053No Hit
TTGCAGTCCTGAGGGGATCTTCTGGCAGAGGTGTGGGTAGGAAGCTGAGT30.1053001053001053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT30.1053001053001053No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTGATACCACTGCTTTGATACCACTGCTTTGATACCACTGCTTTGAT30.1053001053001053No Hit
AGGCCCAGGTCAAGCGCCTTGGTTTGCCCACTAGGATTGTTTTAAGAAAA30.1053001053001053No Hit
CCTCTACAAACCTAGCATTTAAAAAAAAAAAAAAAAAGTACTCTGCGTCT30.1053001053001053No Hit
TCAACGCAGAGTACATGGGAGAGGCTGCAGTGAGCCAAGATTGTGCCATT30.1053001053001053No Hit
ACTCAGAGCCCTGAGCTGGCCAGGAGGCAGAGAGCATCCTCTCTGAGCAG30.1053001053001053No Hit
TTGTACTTCTAGCAAGTGCACCAACAGAAAAACAACGCCTGGAATGGGTG30.1053001053001053No Hit
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA30.1053001053001053No Hit
AATTACAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT30.1053001053001053No Hit
GAGCCCAGGAGATACCATCCTTCCGGGAGGACTTTAGAGCCCATTGTGTG30.1053001053001053No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAATGAGATACCTCATTTTAATTGTTTTTAAGTGTAATTTTAACTAT30.1053001053001053No Hit
CCAAAGAACAAAGTGATGAAAGCACAAAGAGAGCCTGTAGAAATTCCCCA30.1053001053001053No Hit
GTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTAC30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTTTCACCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATC30.1053001053001053No Hit
ACCTGTTACAGATCTCTTCAAACATCTCTCTCCCTGAGGAAATTCCCTGA30.1053001053001053No Hit
GATAAATACCAGAGCATGTGTAATTTCTAGTTCTGCTGTTTCAAGGCTGT30.1053001053001053No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1053001053001053No Hit
CCCCTATGAAGTGTCCTACAGAATTGGAGAGGATGGCTCCATCTGTGTGC30.1053001053001053No Hit
GTGATAGGAACTTTGGACCTTGAACTTACGTATCATGTGGAGAAGAGCCA30.1053001053001053No Hit
TTACAAATTGGTAAAGCATGAACTGGTGTCAACTTGTTCCATTTTAAACT30.1053001053001053No Hit
GGTATCAACGCAGAGTACATGGGACATACTCCTCAGACGATGCACACTTT30.1053001053001053No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT30.1053001053001053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT30.1053001053001053Clontech SMART CDS Primer II A (100% over 26bp)
TGGGAAGATTGCTTAAGACCAGTTCTGGCAACATAGTGAGATCCTCATCT30.1053001053001053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.1053001053001053No Hit
GAGATGTGTATAAGAGACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1053001053001053No Hit
GGCTGTGAATCATGGCACTTGAGAACTGAAAGGTTGTCGCCTTTGCCTAG30.1053001053001053No Hit
ATGGAATGGAGTGGAATGGAATGGAAAGGAATGGAACAATCCGAATGGAA30.1053001053001053No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAAAAAAAA30.1053001053001053No Hit
CACCTAGGTTGGAGTGTAGTGGCACGATCTCGGCTCACTGCAACCTCCAC30.1053001053001053No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAGGCCTAGAAATTCTAATACAATAAGATTAGGGTGGAGTCCAATA30.1053001053001053No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGTACATGGG30.1053001053001053No Hit
GTCTCTGAAAGACCTTTCTCTGGAAGAGGAGTCTGCATTGTAGTGTCTCA30.1053001053001053No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATGGTCTTGATCTCCTGACCTCGTGATCTGCCCGCGTCAGCCTCCCAA30.1053001053001053No Hit
GCCATTCTCCTGCCTCAGCCTCCCGTGTAGCTGGGACTACAGGCGCGCGC30.1053001053001053No Hit

[FAIL]Adapter Content

Adapter graph