FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA953.3345033345033346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT923.2292032292032293Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG381.333801333801334Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG341.1934011934011934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC311.088101088101088Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG260.9126009126009126Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.7020007020007021No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC190.666900666900667Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT190.666900666900667Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.666900666900667Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC160.5616005616005616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC140.4914004914004914Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG140.4914004914004914Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG140.4914004914004914Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC130.4563004563004563Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA110.3861003861003861Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG110.3861003861003861Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA110.3861003861003861Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT100.35100035100035104Clontech Universal Primer Mix Long (100% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA100.35100035100035104Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.35100035100035104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT100.35100035100035104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC90.31590031590031586Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA90.31590031590031586Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG80.2808002808002808Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC80.2808002808002808Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC80.2808002808002808Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA70.2457002457002457Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.2106002106002106Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCC60.2106002106002106Clontech SMART CDS Primer II A (100% over 26bp)
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA60.2106002106002106No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT60.2106002106002106Clontech SMART CDS Primer II A (100% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60.2106002106002106Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA60.2106002106002106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60.2106002106002106Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2106002106002106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50.17550017550017552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA50.17550017550017552Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA50.17550017550017552No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA50.17550017550017552No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC50.17550017550017552Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.17550017550017552No Hit
CAAGAAAAGATCGGACTCACTCATAGACCTAAGGACAAATTGAATTCAGT50.17550017550017552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.17550017550017552No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1404001404001404Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT40.1404001404001404Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT40.1404001404001404No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT40.1404001404001404No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC40.1404001404001404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA40.1404001404001404Clontech Universal Primer Mix Long (95% over 22bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC40.1404001404001404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG40.1404001404001404No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
CCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGCCAAGTGT40.1404001404001404No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA40.1404001404001404Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA40.1404001404001404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1404001404001404No Hit
ACCTTGGTGAGCACCGTCGGGCAATAGCTGCTTCTGAAACTCACAGCTCT30.1053001053001053No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCAAGTACTCTGCGTTGATA30.1053001053001053Clontech SMART CDS Primer II A (96% over 26bp)
GTCTTGTATAAAAAATTTTGTATGGGCTTGTTTATGTATTTCAGGGAGTT30.1053001053001053No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC30.1053001053001053Clontech SMART CDS Primer II A (100% over 26bp)
GGGGAACAAAAGGAAGATCAAAAGGAGGAAAAACAGTAATAATAGCAGAA30.1053001053001053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA30.1053001053001053Clontech SMART CDS Primer II A (100% over 26bp)
GAACAGAAAGCAGAGATCTTAGATAATGACTTCAAAGGCACAAAATGTTT30.1053001053001053No Hit
ACGCAGAGTACATGGGCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGA30.1053001053001053No Hit
CCTTAGTCCAGCAACACCATTCCGGTCTTGCAACGCCTGTAGCCTTTAAA30.1053001053001053No Hit
AGGTTGTCCTGCAGTCCTGCGTTCTGCCTCTATTCACCACATGGAAGGCG30.1053001053001053No Hit
GACTACCAGTGTGTGCTACCAAGACTGGCTAATTTTTTAACTATTTTGTA30.1053001053001053No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT30.1053001053001053No Hit
ACGCAGAGTACATGGGTCACCTGAGGTCGGGAGTTTGAGACCAGCCTGGC30.1053001053001053No Hit
GCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1053001053001053No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAGAGTAC30.1053001053001053Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACCCACATCGTGCGAAATTCTTCCTTGTCGGGATTTTCTTTGGGTGCTG30.1053001053001053No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30.1053001053001053Clontech Universal Primer Mix Long (96% over 25bp)
GACTGTATCCTGGAGGCAGCTTTCTTGTTTTCCATTGCTTTCTATTCATA30.1053001053001053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT30.1053001053001053No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCAATCCTGGCTGGGCGCAGTGGTTCATGCCTGTCATCACAACACTTT30.1053001053001053No Hit
ATACTGGTTCTGTCAGTGAGTGTTTAATTCCCATTTTACCAATGAGAAGA30.1053001053001053No Hit
GTGAGACTGCTATCAATTTGAATTCTGGTTCTGCCTTTCTCTTGCTTTAG30.1053001053001053No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.1053001053001053Clontech Universal Primer Mix Long (96% over 25bp)
GAGCCTGCTGGTCACAGCCTCCTCTGTCTGCCCTTCACCCCAGTGGCCAC30.1053001053001053No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
GAGATAGGGTTTCACCATGTTGCCCAAGCTGGTCTCGAACTCCTGGATTC30.1053001053001053No Hit
GTGTGGAACTATCCCAGGCTTAGGAGGACGTTTTAGCAACCTTGGCCCCT30.1053001053001053No Hit
GAAATCTACATCAATCAATAATTGAAAAGCATCAAATATCACTTTGTTTG30.1053001053001053No Hit
AAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCA30.1053001053001053No Hit
CTCCAGGACCTTGCCGAAGTTGTTACGCATAATTTCCGTCACCTCGTTCG30.1053001053001053No Hit
CCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAAT30.1053001053001053No Hit
CCATTCTATCTTGTGCCAGATCTTCACAGCTGTGACATGGTTTAAATTCC30.1053001053001053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT30.1053001053001053No Hit
CAGTGGTATCAAAGCAGTGGTATCAAAGCAGTGGTATCAAAGCAGTGGTA30.1053001053001053No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
CAGATCACTCCACCCCTGCACAGAAACCTTCAATAGCTCCCCATTGTCTG30.1053001053001053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC30.1053001053001053Clontech Universal Primer Mix Long (96% over 25bp)
GAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGG30.1053001053001053No Hit
AAATAAATGGTTCCTTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1053001053001053No Hit
GCTGAAGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCAAAGAGA30.1053001053001053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCTACACTCT30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCTGCAAAGGGGCGTCGGACAAGTGCAGCTGCTGCGCCTGATGCTGG30.1053001053001053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA30.1053001053001053No Hit
ACTTAATTAGAAAAATATTATAGGATCTTCAATGAGGTTTGGAGATACCT30.1053001053001053No Hit
GTATAGAAAATGTGTTTAAAAAAGGAACTATACAGAGGCTAAAAGCAACC30.1053001053001053No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.1053001053001053Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT30.1053001053001053Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA30.1053001053001053No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.1053001053001053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTTTGAATTGGATGCTTTTGTGTCAACTCCATCTAGTGCCTCCAGAAG30.1053001053001053No Hit
CCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAA30.1053001053001053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)