FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003658919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003658919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC110.9838998211091234No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT80.7155635062611807No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG60.5366726296958855No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA60.5366726296958855No Hit
CCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCA60.5366726296958855No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT50.4472271914132379No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT50.4472271914132379No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG50.4472271914132379No Hit
CCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCCTGGGCATCCCCGTGAAC40.35778175313059035No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG40.35778175313059035No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC40.35778175313059035No Hit
TGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAG40.35778175313059035No Hit
AGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGG30.26833631484794274No Hit
GGCCCAGAGATTTGGAGAAGCGGATGAAGTTCTCCTTTAGTTCCGAAGTC30.26833631484794274No Hit
GGCTCAAGGACATGGTCGGTGTGCTCGAAGCCTTCGTGCGGTACGTGCAG30.26833631484794274No Hit
TCCATGACCAGTGTCCCACTGTGGTCAAAAAACTTAGCAATGGACTTTGT30.26833631484794274No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA30.26833631484794274No Hit
GTATTTATTTTCCCTCGGGTAACTTTTCTATGGCTTCACCATTTTCTCTT30.26833631484794274No Hit
CTTTCCGTCAAGATTTAAATTCCAGGTTGTCATCAATGGCCGAGAGTTTC30.26833631484794274No Hit
TCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAAC30.26833631484794274No Hit
CCTTAGCACCATTGATGAACTTTTCTAAGAGCACTTCTCTTTCAATTTCT30.26833631484794274No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG30.26833631484794274No Hit
GGACAAGCTCAATAACTTAGTCTTGTTTGACAAAGCTACCTATGATAAAC30.26833631484794274No Hit
GCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCT30.26833631484794274No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA30.26833631484794274No Hit
CAACAGTACTGTATGCAGAATGCCTGCAAGGATGCCCTGCTGGTGGGTGT30.26833631484794274No Hit
ACTTTGTAGTCCCTCTTCCACTGCATTTCGGCTTCATACATGCACATGAT30.26833631484794274No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG30.26833631484794274No Hit
GAACTGAGCTTGGTCATTCTCTTAATTAACTCCTGTCCAAAGTGATGATG30.26833631484794274No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG30.26833631484794274No Hit
GCTGTAGCTTACTGAAGTCGCCAAGCAGGAGAGATTTAACCAGAGGCGAT30.26833631484794274No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG30.26833631484794274No Hit
GATCCCATTCAGTGCAGTGTCGTCGCCAGGAATGCCTTGGGGAGGCTCCA30.26833631484794274No Hit
GCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGGTTGGCCAGGG30.26833631484794274No Hit
GGCATCGCTGATCGCTTCTGCAGCCCAGACACCCCCAGGTCCCCTTTTGG30.26833631484794274No Hit
GTATCCCTGCCCCGAGGGCCTCATAGCCAGGTCTCCTGAGAGCAGAGTGA30.26833631484794274No Hit
CAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCC30.26833631484794274No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG30.26833631484794274No Hit
GCTCCTGACTCACCGCTGTTCGCTCTCGCCGAGGAACAAGTCGGTCAGGA30.26833631484794274No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC30.26833631484794274No Hit
GAGGAAAACCGTGTACTATTAGCCATGGTCAACCCCACCGTGTTCTTCGA20.17889087656529518No Hit
CATCTTGACCAGGGAGGCCGCTATGCCAAAGTACACGCAGGCGGCGGCGC20.17889087656529518No Hit
TCTTGGTCCTATTTCCTCCTGTGGCCCGGAGTTTGATGTGTAGGGCGGTG20.17889087656529518No Hit
GCACAAAGTAGATGTCCTAACCGACTTGCCAAAACTATCATTTGTTAACA20.17889087656529518No Hit
GGCTTTACGAGGGCCTTGATAGCCTCGGCACGTGCACTCATGGCCTTGGC20.17889087656529518No Hit
GGTTGAGGGTGCCGAGGGGGTTGGCCAGGGTCCCGGCCCCGGCCTCCGCC20.17889087656529518No Hit
CGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCA20.17889087656529518No Hit
CCTCTAGTTGTCTTATAGGGAGGTGGTCGCCAGGAGGTGGTTGTATAAGG20.17889087656529518No Hit
CCCGTGACTGACAGGAACACAGAGGTGCCCACATGCTGGAAGCGCACAGC20.17889087656529518No Hit
AGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATCAGATGCGTGACA20.17889087656529518No Hit
GACCCCAATCACTTCCGACCTGCTGGCCTGCCTGAGAAATACTGAGCTTC20.17889087656529518No Hit
GCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGAC20.17889087656529518No Hit
CAGCAGTGCTTCCAGCTTCATGGTTCCCAGGTTCAGGTTCCTCCCAGCGG20.17889087656529518No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA20.17889087656529518No Hit
CTGCCACGCTGGGGGCAGCTGGCCTCCGCCTCCGCCCACGCACCAGCAGC20.17889087656529518No Hit
GCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAG20.17889087656529518No Hit
GGTATCAACGCAGAGTACATGGGGAGGTTCCCCGCGCGCCCCGAGCCCCC20.17889087656529518No Hit
CGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAG20.17889087656529518No Hit
GGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG20.17889087656529518No Hit
GTCCCCTTCCCCATTGCCAGCCCCTGACCCTGCACCTAAGTCTGAGCCAG20.17889087656529518No Hit
GTACTGAGTTGTTTGAATAGAACTATGCAAAGCTACAGAGGACATCACAA20.17889087656529518No Hit
GTTCCGTCCAAACACTGTTGAAGCAAGAGACAGACCCGCGGGACCGCCGA20.17889087656529518No Hit
CCTTCTTGCCCTTCTCCTTCTTCACTTTGGGCTTCTTGGCCTTGCCCGGG20.17889087656529518No Hit
CCTTCATTCTCTTGGCCAAAAGTTTAGCATATTCTGCAGCCTCTTCTTTA20.17889087656529518No Hit
GGGTACGTGAACACCTACGCCCAACACCTCAAGCCGTTTCAAAACGGAAA20.17889087656529518No Hit
GGTTGGGGAAGATGCGGCGCGTGTCCGGCACCAGCGCCACGCCGAGCTTG20.17889087656529518No Hit
CCTCAGCCAGCTGCTGAGCCTTGCTCACCTCCAGCTCCAGCTGGGCCCGG20.17889087656529518No Hit
ACTACCGTTTGTGCATGGCTAAAGACCGTGGTGATTTTATAGCATCCTGG20.17889087656529518No Hit
CCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGC20.17889087656529518No Hit
TTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG20.17889087656529518No Hit
GTAGGCGAATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGT20.17889087656529518No Hit
CATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGG20.17889087656529518No Hit
CTGCTGAGCAGCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCA20.17889087656529518No Hit
CTCCCATGGCTGGTGGCACAGTCACTGAGGACCAGGCGGGTGGGGCCACT20.17889087656529518No Hit
CTGGCCGATCAGGCTGCCAATGAATGGGGCAGGAGTGGCAAAGACCCCAA20.17889087656529518No Hit
GCGCGATGGCGGGGAGTAGCTCGCTGGAGGCGGTGCGCAGGAAGATCCGG20.17889087656529518No Hit
GCCATATTCTCCACATTTGGAGCTCTGTCAGGCTGCTCCCTTTCCACCAG20.17889087656529518No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG20.17889087656529518No Hit
GTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGG20.17889087656529518No Hit
ATCTACCACCTTTGTGTTGGGATCTCGAGCCAACAAAGCCCTGGGGATGG20.17889087656529518No Hit
TGAATATACTACGGAGAGTTTGTAGAAAGTAAACTTTAAAATCCCACACT20.17889087656529518No Hit
GCAAGGAGGCGGATGAGGAACAATGCAAAGATGGCTTTTCAGAGCAGCCA20.17889087656529518No Hit
GGCCGAGCCCACTAAGAAAGCAGCAGCGGAGCTGCAGGACAGGGCCACGC20.17889087656529518No Hit
GGCTTCCTCCTTGTTGGGTTTGGGGTGTCAACAGGATCCAGGCATTTGAT20.17889087656529518No Hit
CATCTCGGGCCTGGGAGAGGGAGGGCGTGGGCCAGGCCCCATGTGACACC20.17889087656529518No Hit
CCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGAC20.17889087656529518No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT20.17889087656529518No Hit
GTCCCCGACGGCTACGGTGTCTGCTATAACCCCATGGAGGCCCACATCAA20.17889087656529518No Hit
GGAAACTTGAACTTGGCCCTGCGCAGGGCCTCAATCACATGCTCCTTGTT20.17889087656529518No Hit
TGCCACTAGGACTGAAGTCCCGCATCAAGATCCAGCTTGGCAGCTCCCCC20.17889087656529518No Hit
ATTGTAAATGGTGGATGACAAAAGAAAATCTGCTCTGTGGAAAGAAAGAA20.17889087656529518No Hit
GTGTGGTAGCGGGTGTCGTCGTAGTCCAGGTACTCCCGGAACTGGGACAG20.17889087656529518No Hit
GAGTTGCCTCGGGCAGCCTTGTGGCTACTCTGCAGTCACTGGGAGCAACT20.17889087656529518No Hit
CAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCT20.17889087656529518No Hit
GAAATATCTCACCAAAAAATATTTGAAGAAGAATAATCTACGTGACTGGT20.17889087656529518No Hit
GATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGT20.17889087656529518No Hit
GCAGTGGTGCAATCTTGTGTCACTGCAACCTCTGCCTCCTAAGTTAAAGT20.17889087656529518No Hit
GTCTCGCTCTGTCACCCAGGCTGGACTGCAGTGGCGTGATCTCGGCTCAC20.17889087656529518No Hit
GATAAAACACTACAAACTGCGGCACCACGCGAAAGAAAGACTCCCGAATG20.17889087656529518No Hit
GGCTAGAGCTCTGTGATGAGCGTGTATCCTCTCGATCACATACAGAAGAG20.17889087656529518No Hit
GCTTTTGATGGGGCTCGGGACATGTGGAGAGCCTACTCTGACATGAGAGA20.17889087656529518No Hit
CTTGTAAGGAGCCTCAAGCATTGCTTCAATATCAATATCGTCTGCCATTT20.17889087656529518No Hit
GGGGTCGCCTCCTTCCCGCTTGCAGACCTCCGCGAAGTCCTCCTTGCTCA20.17889087656529518No Hit
ACGCAGAGTACATGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGT20.17889087656529518No Hit
GTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGG20.17889087656529518No Hit
GAGCAGGGTTCTCAGTTGGTGCTTCACCTGCCTTGGCCTCTTTGCCATCT20.17889087656529518No Hit
GCGCCGGCTTCTGCGCCGGCTGGCCTGCCGCGGCCGGTGCGCTCGCCGCC20.17889087656529518No Hit
TGTGTGGGAAATCCTGCGTTTCCCCTGTGAAAGCTTGATTCCTGCCATAT20.17889087656529518No Hit
GTACAGCGCTTGTACCTTCTAGGGGAAGCCTTGCGTGGCTGGGCCCACAG20.17889087656529518No Hit
ATTCGATTCCACTCGATGATTCCATTCGATTCCATTCAATGATGATTCCA20.17889087656529518No Hit
CCCATTCTCTGCCTGTACTGAAGCTGAAATGCAGGAAGTGGTGGCAAAGG20.17889087656529518No Hit
ACGCAGAGTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGC20.17889087656529518No Hit
ATCTTATACTTGGTCACGACCAGGTCCTCAGCGATAGTTTGCTCCTGTTG20.17889087656529518No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT20.17889087656529518No Hit
GTCCTCATGCCCTGCCACCAGCATCTCTACTTCTTCCTCTGTCATCTTCT20.17889087656529518No Hit
TGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTGGTT20.17889087656529518No Hit
GGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAG20.17889087656529518No Hit
GGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCC20.17889087656529518No Hit
ATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTA20.17889087656529518No Hit
GTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGC20.17889087656529518No Hit
AAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCA20.17889087656529518No Hit
GCCCTGTTGTGAAATATGTGATTCCAGTTCAATTCAGAGTGTATGATTCC20.17889087656529518No Hit
TCTCTTTTCTGCTTTCGTAACTGCAGTCCTTCTTCCTCCCTCCTCCTGCG20.17889087656529518No Hit
CGCGAATTCCACGTGCTAGGCCATCGTGGATGAGGGCAGTCTTCAGAACC20.17889087656529518No Hit
GCACTGCAGAGGCATCAGCCGGGCTGAGTCATGCCTGAGATCGAAGGGCC20.17889087656529518No Hit
GTATCAACGCAGAGTACATGGGCTGCTTGCTGCAGTAGTAACCTCAAGTG20.17889087656529518No Hit
TTCCCACACATGCCCATGCAACACTTCAAGTCACGCTTGCACTGGCCATC20.17889087656529518No Hit
CACTTCACTTGGCATCTCCAGCACCTTCAGCTTTCTGTGCCTGGTCTGTT20.17889087656529518No Hit
GTACCCGCCACACTGCATCTGCTGCCCTGCGAGGTTGCGGTGGACGGACC20.17889087656529518No Hit
GCTCTAAGGCCTACCACCTTCAGAAGTCGACCTGTGGCAAATGTGGCTAC20.17889087656529518No Hit
GAACTAAGGTGGCTAATCAGCTGACCTGAAAATAGAAAGACGACCTGGGT20.17889087656529518No Hit
GCTCTCGACGCCCTGCGCGCCTGCTGATGCCTAGCCAGTTGGTAAGCCAG20.17889087656529518No Hit
ACTTGTACCAGCATCCCCAGCGTCTGGCATTCCATGTTTCTGCTCCTGTG20.17889087656529518No Hit
ATGCAGGGCCAGGCAGAAACTTTCTCTCCTCACTGCTCAGCCTGGTGGTG20.17889087656529518No Hit
CGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGCGTCGTGTAGG20.17889087656529518No Hit
CTTTGATGCCTTTGGTGAAAAGGAAACGTCTTCCAATAAAAGCCAGACAG20.17889087656529518No Hit
ATGGTGGCCTACTGGAGACAGGCTGGACTCAGCTACATCCGATACTCCCA20.17889087656529518No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC20.17889087656529518No Hit
GTATATAAGATTACTTTATTCCTGCATCTTCTCAATGGTTTCTTCCTTGT20.17889087656529518No Hit
CTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGGGGGCCGTGA20.17889087656529518No Hit
TCCTGATTTGGACAAGCAGCAATTTGTAAGTGTCCCCGTTCCTTATTGAA20.17889087656529518No Hit
GTCCTCGGTCACCCCCTGGGCAGCCAGCTCAGCCAGCACGTTCTGCAGGT20.17889087656529518No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC20.17889087656529518No Hit
GAATACAGAGTCCCGAAAAAGGGGAGGGCAGGTGGGCTGGAACCAGATGC20.17889087656529518No Hit
ACCAACCAGCTTGTAGATTTTCAGTGGAAACTGGGTATGGCTGTGAACTC20.17889087656529518No Hit
CCTCATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCG20.17889087656529518No Hit
GTCTGGAAGCGGCCATGGCCAAACTTGGAGGTGGTGTCAATGAACTTAAG20.17889087656529518No Hit
GGCTTGGCCGAGCCCACTAAGAAAGCAGCAGCGGAGCTGCAGGACAGGGC20.17889087656529518No Hit
GTCCCGGCCCCGGCCTCCGCCGCCGACTCCAGCGCAGCGACAGGCTGGGC20.17889087656529518No Hit
GGTCCCGGTAGATAATCATGATGGCGACTGAAGGGAGACGACGACGGCGC20.17889087656529518No Hit
ATGCCATCCTGTCAGATCAGTTCCGGGTCCCCGCAGGGGGGTCCCGGTTC20.17889087656529518No Hit
GTGCTGGGGGCACCCCCCAGCACCATCCTTCCAAGGTCCACCGTGATCAA20.17889087656529518No Hit
GTCTAGGCTGGGACCCTTTCCAGAGGTAAGTCAAGGTCACGTCCCTGCCC20.17889087656529518No Hit
GGTCTACGCCTATTACAACCTGGAGGAAAGCTGTACCCGGTTCTACCATC20.17889087656529518No Hit
CACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCT20.17889087656529518No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAAT20.17889087656529518No Hit
GGTCTCTCCGACACTGGGACAGCTTCTCCTCTAGCCCATCGATCTCTAGA20.17889087656529518No Hit
GTGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGG20.17889087656529518No Hit
ACCCTAAACCACAAGATGGCAAAGAGACAAAAGCAGCCTGTCTCTTATAC20.17889087656529518No Hit
AGCCAGCAGTCCCGCCAGAAGAGGCTGAGCCACACACTGAGCCCGAGGAG20.17889087656529518No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC20.17889087656529518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)