FastQCFastQC Report
Tue 10 May 2022
EGAF00003659021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15368
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1530.995575221238938No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT830.5400832899531494No Hit
GTAGTAGACAGCCTTGCTCGTGGTTGTTGCGGACGTTGCGCTTCCGGCCG460.2993232691306611No Hit
GTACATGGGTGAGCCAAGACCCCACCTTTGCACTCCAGCCTGGGCAACAA290.18870380010411245No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA280.18219677251431546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGG270.17568974492451847No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT250.16267568974492452Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.16267568974492452No Hit
CTCCCAGACATTGCAGATGCTGTCATACCTTTCCATGGAGCCCATGAGCT240.15616866215512754No Hit
ATTATAAATAGTGCTGCTTTGCTTGGTGCGGTTGCTCACACCTGTAATCC240.15616866215512754No Hit
CATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGGATA220.14315460697553356No Hit
GTGTTGGAAGTGGGGGCTAGTGGGAGGTGTTTGGGTCATGGGGACAGATC210.1366475793857366No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG200.1301405517959396No Hit
GTCTTGTTCTGTCCCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCAA200.1301405517959396No Hit
GTCTACTACAACGCCCTGTTCGACGTGCCCAACGACCACGGAATCCTGCG200.1301405517959396No Hit
TCTCAGTACAGTGCTCTTCCAAGATAAGGTTGGACTTTGCGCCGGTCATC200.1301405517959396No Hit
GTCTACATCTTCTCCCTGAAACTCAATCAGTGGAGGCTGGGGGAGCCCAT190.12363352420614263No Hit
TCATTACACAGTGGCTGCTATAGAACCCCCAGACTTCTGTGAACACAGTT190.12363352420614263No Hit
GTATGTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGC190.12363352420614263No Hit
CTCTATTGCCATGTGACATGCCCGCTCCCATTCGCCTTCTGCCATGATTG180.11712649661634565No Hit
TCACTATCCAGCAGGGTCTATCCATTCAGGTGCTCCAAGTGCCTGCCAGC180.11712649661634565No Hit
GGCCCAGGTCGTGCAGGCGCGAACGCAGGTCGACACGGCTCGCACGCAGC180.11712649661634565No Hit
TGTGATGACCGTGCGCGCCGAGATCTCCGAGGCCGACGTGAACCTCGTGG180.11712649661634565No Hit
TCCTAGTATTCAGGCCCTTGTGGACTGCCCTCACACAGAGTCAGGACTGA180.11712649661634565No Hit
CCATAGAAGAAGCCCAGTTTCCCTGAGGCTATCATGCTGGAGAGATCACA180.11712649661634565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11061946902654868No Hit
GTATCAACGCAGAGTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCC170.11061946902654868No Hit
GTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACACATACTCAT170.11061946902654868No Hit
GACCTGGACCGTCATCGATGGACGCAGGATTCCGTGGTCGTTGGGCACGT160.10411244143675169No Hit
GTGTGAATAGAATGTGGTAGGAATTATGTTCTGGGACTTTCCAAGCTGGG160.10411244143675169No Hit
CCTTAGAAATGTCCCCAGGAACCAAAGTGAGGAGATTCCATTGGATGTCA160.10411244143675169No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT160.10411244143675169No Hit
ATCTTGGAGACCGCCAAGAGACAGATGTTCTCTTTGTGTGGCACCACCGT160.10411244143675169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)